ST1074
Based on 1 Customer Validation
ST1074 is a ceramide synthase inhibitor targeting CerS2 and CerS4. ST1074 has human IC50 values of 28.7 μM and 21.4 μM for CerS2 and CerS4, respectively, and reduces the synthesis of specific ceramide products. ST1074 induces apoptosis and decreases cell viability. SST1074 can be used for research on cancer and melanoma.
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- 純度 : 99.41%
- CAS 番号: 1402703-29-0
- 分子式: C20H36ClNO3
- 分子量:373.96
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保管条件:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- プロトコル
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
製品説明
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CerS2 28.7 μM (IC50) |
CerS4 21.4 μM (IC50) |
体外実験
ST1074 (10 mM; 10 min preincubation; 30 min reaction) inhibits CerS2 and CerS4 in HCT-116 lysates overexpressing individual CerS isoforms[1].
ST1074 (5 mM; 2 h) inhibits CerS2/4-dependent dihydroceramide and ceramide species in HCT-116/HeLa cell-based assays[1].
ST-1074 (5-10 µM) inhibits CerS2 and CerS4 in HCT116 cells at 10 µM with IC50 values of 28.7 µM for C24:1/CerS2 and 21.4 µM for C18:0/CerS4, and inhibits CerS2 and CerS4 in HeLa cells at 5 µM[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HCT-116 cells
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Concentration:10 μM; 20 μM; 30 μM; 40 μM; 50 μM; 60 μM; 70 μM; 80 μM
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Incubation Time:48 h
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Result:Exhibited cytotoxicity against HCT-116 cells.
- Detection of Apoptosis
- TUNEL staining for apoptotic DNA fragmentation
- Cell Cytotoxicity Assay
- Mammalian live/dead viability and cytotoxicity staining
- Annexin V plus membrane-impermeant dye apoptosis staining
- Apoptosis Solutions
- CCK-8/WST-8 Cell Proliferation Assay
- MTT Cell Proliferation Assay
- Cell Viability Determination by MTT Colorimetric Assay
化学情報
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CAS 番号 1402703-29-0
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性状 Solid
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分子量 373.96
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分子式 C20H36ClNO3
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Color White to off-white
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SMILES
OCC(CO)(CCC1=CC=C(C=C1)OCCCCCCC)N(C)C.Cl
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
溶剤 & 溶解度
体外:
DMSO : 100 mg/mL (267.41 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
体内:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.69 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.69 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション
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データシート (273 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.6741 mL | 13.3704 mL | 26.7408 mL | 66.8521 mL |
| 5 mM | 0.5348 mL | 2.6741 mL | 5.3482 mL | 13.3704 mL | |
| 10 mM | 0.2674 mL | 1.3370 mL | 2.6741 mL | 6.6852 mL | |
| 15 mM | 0.1783 mL | 0.8914 mL | 1.7827 mL | 4.4568 mL | |
| 20 mM | 0.1337 mL | 0.6685 mL | 1.3370 mL | 3.3426 mL | |
| 25 mM | 0.1070 mL | 0.5348 mL | 1.0696 mL | 2.6741 mL | |
| 30 mM | 0.0891 mL | 0.4457 mL | 0.8914 mL | 2.2284 mL | |
| 40 mM | 0.0669 mL | 0.3343 mL | 0.6685 mL | 1.6713 mL | |
| 50 mM | 0.0535 mL | 0.2674 mL | 0.5348 mL | 1.3370 mL | |
| 60 mM | 0.0446 mL | 0.2228 mL | 0.4457 mL | 1.1142 mL | |
| 80 mM | 0.0334 mL | 0.1671 mL | 0.3343 mL | 0.8357 mL | |
| 100 mM | 0.0267 mL | 0.1337 mL | 0.2674 mL | 0.6685 mL |
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