TPCK-treated Trypsin
Based on 1 publication(s) in Google Scholar
TPCK-treated Trypsin is a trypsin whose activity is inhibited by tosyl phenylalanyl chloromethyl ketone. TPCK-treated Trypsin reduces autolysis and nonspecific proteolysis during experiments, exhibits stability in storage and handling. TPCK-treated trypsin can be used in proteomics research. TPCK-treated Trypsin renders the virus hemagglutinin active, which allows multicycle replication of the virus. TPCK-treated Trypsin can be used for the study of influenza virus.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- CAS 番号: 9002-07-7
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
MedChemExpress（MCE）の使用を引用している文献 TPCK-treated Trypsin
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生物活性
Product Information This product is derived from bovine pancreas. After chromatographic purification, it is treated with L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK) to inhibit the activity of proteases. The theoretical molecular weight is 23.3 kDa, and the optimal pH is 7.5-8.5. It can be used for protein analysis, such as protein mass spectrometry, sequencing, and peptide mapping analysis. Instructions (for reference only) This product can be directly used for protein enzymatic cleavage. The recommended enzymatic cleavage ratio is 1:50-1:100 (w/w); Enzymatic cleavage overnight at 37°C.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
3.4.21.4
≥180 U/mg protein
One Unit hydrolyzes one micromole of TAME per minute at 25°C, pH 8.2, in the presence of 0.01M calcium. (One TAME unit = 19.2 USP/NF units = 57.5 BAEE units.)
化学情報
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CAS 番号 9002-07-7
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性状 Solid
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Color White to off-white
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SMILES
[TPCK-treated Trypsin]
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (1)
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Journal Impact Factor
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Most Recent
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Nat Commun
Dipeptidylpeptidase 4 inhibition attenuates gestational pathologies via immune homeostasis restoration in the pulmonary-uterine axis. [Abstract]2026 Feb 17;17(1):2851. PMID: 41702893
溶剤 & 溶解度
H2O : 100 mg/mL (Need ultrasonic)
純度とドキュメンテーション
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データシート (267 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Shuford CM, et al., Cheaper, faster, simpler trypsin digestion for high-throughput targeted protein quantification. J Mass Spectrom Adv Clin Lab. 2023 Nov 23;30:74-82. [Content Brief]
[2]. Cuevas F, et al. An In Vitro Microneutralization Assay for Influenza Virus Serology. Curr Protoc. 2022 Jul;2(7):e465. [Content Brief]
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
TPCK-treated Trypsin 関連分類
HY-129047C 関連分類
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