Ketone monoester
Based on 2 publication(s) in Google Scholar
Ketone monoester is an orally available ketone monoester that serves as a source of nutritional ketones. Ketone monoester increases plasma beta-hydroxybutyrate, acetoacetate, blood glucose, blood sodium, and blood creatinine levels in mouse models. Ketone monoester has the potential to improve athletic performance and endurance in animals. Ketone monoester partially prevents myasthenia in septic mice. Ketone monoester may also be used to study Parkinson's disease or diabetes.
For research use only. We do not sell to patients.
- Purity: 95.0%
- CAS No.: 1208313-97-6
- Formula: C8H16O4
- Molecular Weight:176.21
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Storage:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Ketone monoester
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Biological Activity
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Septic mice[1]
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Dosage:10, 20, 40 and 80 mmol/kg/day
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Administration:PO
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Result:Increased specific muscle force as compared with placebo to 93% of healthy control levels at 40 mmol/kg/day.
Resulted in moderately higher blood glucose concentrations as compared with placebo at 40 mmol/kg/day.
Lowered Aldh3b2 gene expression than with placebo at 40 mmol/kg/day.
Evoked a moderate further increase in blood Na+ levels and increased blood creatinine levels at 20 mmol/kg/day.
Reduced plasma free fatty acid concentrations by 10 or 20 mmol/kg/day.
Hepatic gene expression levels of Aldh1a7 was also reduced by sepsis but increased by D-3HHB.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 1208313-97-6
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Appearance Liquid (Density: 1.102 g/cm3)
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Molecular Weight 176.21
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Formula C8H16O4
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Color Colorless to light yellow
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SMILES
C[C@@H](O)CC(OCC[C@H](O)C)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (2)
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Journal Impact Factor
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Most Recent
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Pharmaceuticals (Basel)
(R,R)-BD-AcAc2 Mitigates Chronic Colitis in Rats: A Promising Multi-Pronged Approach Modulating Inflammasome Activity, Autophagy, and Pyroptosis. [Abstract]2023 Jul 3;16(7):953. PMID: 37513865 -
Toxicol Appl Pharmacol
Tracking the therapeutic efficacy of a ketone mono ester and β-hydroxybutyrate for ulcerative colitis in rats: New perspectives. [Abstract]2024 May:486:116943. PMID: 38677600
Solvent & Solubility
H2O : 100 mg/mL (567.50 mM; Need ultrasonic)
DMSO : 100 mg/mL (567.50 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5.5 mg/mL (31.21 mM); Clear solution
This protocol yields a clear solution of ≥ 5.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (55.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5.5 mg/mL (31.21 mM); Clear solution
This protocol yields a clear solution of ≥ 5.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (55.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (567.50 mM); Clear solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (272 KB)
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SDS (476 KB)
- English - EN (476 KB)
- Français - FR (476 KB)
- Deutsch - DE (476 KB)
- Norwegian - NO (476 KB)
- Español - ES (476 KB)
- Swedish - SV (476 KB)
- Italian - IT (476 KB)
- Portuguese - PT (476 KB)
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Handling Instructions (2659 KB)
References
[1]. Weckx R, et al. Efficacy and safety of ketone ester infusion to prevent muscle weakness in a mouse model of sepsis-induced critical illness. Sci Rep. 2022 Jun 22;12(1):10591. [Content Brief]
[2]. Williams MS, et al. The Chemistry of the Ketogenic Diet: Updates and Opportunities in Organic Synthesis. Int J Mol Sci. 2021 May 15;22(10):5230. [Content Brief]
[3]. Clarke K, et al. Oral 28-day and developmental toxicity studies of (R)-3-hydroxybutyl (R)-3-hydroxybutyrate. Regul Toxicol Pharmacol. 2012 Jul;63(2):196-208. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 5.6750 mL | 28.3752 mL | 56.7505 mL | 141.8762 mL |
| 5 mM | 1.1350 mL | 5.6750 mL | 11.3501 mL | 28.3752 mL | |
| 10 mM | 0.5675 mL | 2.8375 mL | 5.6750 mL | 14.1876 mL | |
| 15 mM | 0.3783 mL | 1.8917 mL | 3.7834 mL | 9.4584 mL | |
| 20 mM | 0.2838 mL | 1.4188 mL | 2.8375 mL | 7.0938 mL | |
| 25 mM | 0.2270 mL | 1.1350 mL | 2.2700 mL | 5.6750 mL | |
| 30 mM | 0.1892 mL | 0.9458 mL | 1.8917 mL | 4.7292 mL | |
| 40 mM | 0.1419 mL | 0.7094 mL | 1.4188 mL | 3.5469 mL | |
| 50 mM | 0.1135 mL | 0.5675 mL | 1.1350 mL | 2.8375 mL | |
| 60 mM | 0.0946 mL | 0.4729 mL | 0.9458 mL | 2.3646 mL | |
| 80 mM | 0.0709 mL | 0.3547 mL | 0.7094 mL | 1.7735 mL | |
| 100 mM | 0.0568 mL | 0.2838 mL | 0.5675 mL | 1.4188 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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