KH-4-43

Name: KH-4-43
Brand: MedChemExpress
SKU: HY-148602
Rating: 4.5 (1 reviews)

Cat. No.: HY-148602 Purity: 99.99%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

KH-4-43 is an inhibitor of E3 CRL4. KH-4-43 inhibits E3 CRL4's core ligase complex and exhibits anticancer activity. KH-4-43 has a binding K_d to E3 ROC1-CUL4A CTD or the highly related ROC1-CUL1 CTD at 83 nM or 9.4 μM, respectively.

For research use only. We do not sell to patients.

KH-4-43 Chemical Structure

CAS No. : 2813310-07-3

Size	Price	Stock	Quantity
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 638	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 638	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 580	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 950	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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This product is a controlled substance and not for sale in your territory.

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Based on 1 publication(s) in Google Scholar

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Description

Molecular Weight

422.74

Formula

C₁₉H₁₀ClF₃N₂O₄

CAS No.

2813310-07-3

Appearance

Solid

Color

Off-white to yellow

SMILES

O=C1C2=CC=C(C(O)=C2OC(C(F)(F)F)=C1C3=CN(C4=CC=C(C=C4)Cl)N=C3)O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (236.55 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.3655 mL	11.8276 mL	23.6552 mL
5 mM	0.4731 mL	2.3655 mL	4.7310 mL
10 mM	0.2366 mL	1.1828 mL	2.3655 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.91 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.99%

Select Batch:

Data Sheet (271 KB) SDS (0 KB)

COA (249 KB) HNMR (264 KB) LCMS (299 KB)

Handling Instructions (2659 KB)

References

[1]. Wu K, et al. Targeting Cullin-RING E3 Ubiquitin Ligase 4 by Small Molecule Modulators. J Cell Signal. 2021;2(3):195-205. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.3655 mL	11.8276 mL	23.6552 mL	59.1380 mL
	5 mM	0.4731 mL	2.3655 mL	4.7310 mL	11.8276 mL
	10 mM	0.2366 mL	1.1828 mL	2.3655 mL	5.9138 mL
	15 mM	0.1577 mL	0.7885 mL	1.5770 mL	3.9425 mL
	20 mM	0.1183 mL	0.5914 mL	1.1828 mL	2.9569 mL
	25 mM	0.0946 mL	0.4731 mL	0.9462 mL	2.3655 mL
	30 mM	0.0789 mL	0.3943 mL	0.7885 mL	1.9713 mL
	40 mM	0.0591 mL	0.2957 mL	0.5914 mL	1.4785 mL
	50 mM	0.0473 mL	0.2366 mL	0.4731 mL	1.1828 mL
	60 mM	0.0394 mL	0.1971 mL	0.3943 mL	0.9856 mL
	80 mM	0.0296 mL	0.1478 mL	0.2957 mL	0.7392 mL
	100 mM	0.0237 mL	0.1183 mL	0.2366 mL	0.5914 mL

KH-4-43 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

KH-4-432813310-07-3OthersAcute Myelogenous LeukemiaInhibitorinhibitorinhibit

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KH-4-43

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Complete Stock Solution Preparation Table

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