Diuron-d6
Based on 1 Customer Validation
Diuron-d6 is the deuterium labeled Diuron (HY-B0860). Diuron is an orally active phenylurea herbicide. Diuron inhibits photosynthesis in plants by blocking the formation of ATP and NADH. Diuron increases the production of ROS. Diuron increases expression of p53 in certain cell lines. Diuron has herbicidal activity against annual and perennial broadleaf weeds and grass weeds. Diuron promotes DMBA/BBN-induced bladder cancer. Diuron can be used in breast cancer research.
For research use only. We do not sell to patients.
- Purity: 99.66%
- CAS No.: 1007536-67-5
- Formula: C9H4D6Cl2N2O
- Molecular Weight:239.13
-
Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
1. This compound can be used as a tracer
2. This compound can be used as an internal standard for quantitative analysis by NMR, GC-MS, or LC-MS.
Chemical Information
-
CAS No. 1007536-67-5
-
Unlabeled Cas 330-54-1
-
Appearance Solid
-
Molecular Weight 239.13
-
Formula C9H4D6Cl2N2O
-
Color White to off-white
-
SMILES
[2H]C([2H])([2H])N(C([2H])([2H])[2H])C(NC1=CC(Cl)=C(C=C1)Cl)=O
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : ≥ 100 mg/mL (418.18 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (10.45 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
-
Data Sheet (275 KB)
-
SDS (761 KB)
- English - EN (761 KB)
- Français - FR (761 KB)
- Deutsch - DE (761 KB)
- Norwegian - NO (761 KB)
- Español - ES (761 KB)
- Swedish - SV (761 KB)
- Italian - IT (761 KB)
- Korean - KR (761 KB)
- Portuguese - PT (761 KB)
-
Handling Instructions (2659 KB)
References
[1]. Huovinen M, et al. Toxicity of diuron in human cancer cells. Toxicol In Vitro. 2015 Oct;29(7):1577-86. [Content Brief]
[2]. Lundegårdh H. THE INFLUENCE OF DIURON (3-(3,4-DICHLOROPHENYL)-1,1-DIMETHYLUREA) ON THE RESPIRATORY AND PHOTOSYNTHETIC SYSTEMS OF PLANTS. Proc Natl Acad Sci U S A. 1965 Apr;53(4):703-10. [Content Brief]
[3]. Da Rocha MS, et al. Diuron-induced rat urinary bladder carcinogenesis: mode of action and human relevance evaluations using the International Programme on Chemical Safety framework. Crit Rev Toxicol. 2014 May;44(5):393-406. [Content Brief]
[5]. Da Rocha MS, et al. Diuron-induced rat bladder epithelial cytotoxicity. Toxicol Sci. 2012 Dec;130(2):281-8. [Content Brief]
[6]. de Moura NA, et al. Potential effects of the herbicide Diuron on mammary and urinary bladder two-stage carcinogenesis in a female Swiss mouse model. Arch Toxicol. 2010 Feb;84(2):165-73. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.1818 mL | 20.9091 mL | 41.8183 mL | 104.5456 mL |
| 5 mM | 0.8364 mL | 4.1818 mL | 8.3637 mL | 20.9091 mL | |
| 10 mM | 0.4182 mL | 2.0909 mL | 4.1818 mL | 10.4546 mL | |
| 15 mM | 0.2788 mL | 1.3939 mL | 2.7879 mL | 6.9697 mL | |
| 20 mM | 0.2091 mL | 1.0455 mL | 2.0909 mL | 5.2273 mL | |
| 25 mM | 0.1673 mL | 0.8364 mL | 1.6727 mL | 4.1818 mL | |
| 30 mM | 0.1394 mL | 0.6970 mL | 1.3939 mL | 3.4849 mL | |
| 40 mM | 0.1045 mL | 0.5227 mL | 1.0455 mL | 2.6136 mL | |
| 50 mM | 0.0836 mL | 0.4182 mL | 0.8364 mL | 2.0909 mL | |
| 60 mM | 0.0697 mL | 0.3485 mL | 0.6970 mL | 1.7424 mL | |
| 80 mM | 0.0523 mL | 0.2614 mL | 0.5227 mL | 1.3068 mL | |
| 100 mM | 0.0418 mL | 0.2091 mL | 0.4182 mL | 1.0455 mL |
Diuron-d6 Related Classifications
HY-B0860S Related Classifications
Powered by Bioz