2614W94
Based on 1 Customer Validation
2614W94 is a selective, reversible and orally active monoamine oxidase-A (MAO-A) inhibitor with an IC50 of 5 nM and a Ki of 1.6 nM. 2614W94 shows no significant inhibition of MAO-B. 2614W94 has antidepressant and anxiolytic effects.
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- Purity: 99.76%
- CAS No.: 205187-35-5
- 화학식: C15H11F3O4S
- 분자량:344.31
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보관:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
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Biological Activity
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Chemical Information
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용액&용해도
- Protocol
- 순도&문서
- References
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Help & FAQs
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Mitochondria-Targeted Compound Library
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Antidepressant Compound Library
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Metabolic Enzyme Compound Library
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Highly Selective Inhibitors Library
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Bioactive Compound Library Max
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MCE Bioactive Compound Library
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Bioactive Compound Library
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High-Throughput Bioactive Compound Library
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Biological Activity
IC50: 5 nM (Monoamine Oxidase)[1]
Ki: 1.6 nM (Monoamine Oxidase)[1]
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 205187-35-5
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Appearance Solid
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분자량 344.31
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화학식 C15H11F3O4S
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Color Off-white to light yellow
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SMILES
CC(OC(C=C1OC2=C3C=CC=C2)=CC=C1S3(=O)=O)C(F)(F)F
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선적
Room temperature in continental US; may vary elsewhere.
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보관
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
용액&용해도
DMSO : 100 mg/mL (290.44 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocol
MAO-A and -B forms are assayed. Rat brain mito-chondrial extract is pre-incubated with the inhibitor for 15 min at 37°C in 50 mM potassium phosphate buffer (pH 7.4). Substrates [3H]serotonin (0.2 mM, 5 Ci/mol) and [14C]β-phene-thylamine (10 µM, 3 Ci/mol) are then added, and incubation at 37°C is continued for 20 min. Blank assays contain 2 mM pargyline to inhibit all MAO activity. The reaction is terminated with 0.2 mL of 2 N HCl, and products are extracted with 6 mL of ethyl acetate/toluene (1:1). A 4 mL aliquot of the organic layer is countedin 10 mL of Ecolite in a scintillation spectrometer programmed for double-label counting. Assays are performed in triplicate unless otherwise indicated. At the above concentrations, serotonin is a selective substrate for MAO-A, and β-phenethylamine is aselective substrate for MAO-B.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Rats: Nonfasted Sprague-Dawley male rats (250-350 g) are dosed by gavage with 0.5% methyl cellulose or with 2614W94 or other compounds suspended in the methyl cellulose vehicle. For all groups, n = 3 unless otherwise specified. For oral administration, dosing volume is 10 mL/kg of body weight. For intravenous dosing, the vehicle is a mixture of PEG 400 (polyethylene glycol; molecular weight, 400), ethanol, and physiologic saline in a volume ratio of 1.5/1.5/1.0, respectively, and the dosing volume is 1 mL/kg. After dosing, rats are returned to their cages and allowed free access to water. Any animals kept overnight are also given food. Death is by CO2 asphyxiation, after which brains and livers are promptly removed, frozen on dry ice, and stored at -70°C.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
순도&문서
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Data Sheet (276 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.9044 mL | 14.5218 mL | 29.0436 mL | 72.6090 mL |
| 5 mM | 0.5809 mL | 2.9044 mL | 5.8087 mL | 14.5218 mL | |
| 10 mM | 0.2904 mL | 1.4522 mL | 2.9044 mL | 7.2609 mL | |
| 15 mM | 0.1936 mL | 0.9681 mL | 1.9362 mL | 4.8406 mL | |
| 20 mM | 0.1452 mL | 0.7261 mL | 1.4522 mL | 3.6304 mL | |
| 25 mM | 0.1162 mL | 0.5809 mL | 1.1617 mL | 2.9044 mL | |
| 30 mM | 0.0968 mL | 0.4841 mL | 0.9681 mL | 2.4203 mL | |
| 40 mM | 0.0726 mL | 0.3630 mL | 0.7261 mL | 1.8152 mL | |
| 50 mM | 0.0581 mL | 0.2904 mL | 0.5809 mL | 1.4522 mL | |
| 60 mM | 0.0484 mL | 0.2420 mL | 0.4841 mL | 1.2101 mL | |
| 80 mM | 0.0363 mL | 0.1815 mL | 0.3630 mL | 0.9076 mL | |
| 100 mM | 0.0290 mL | 0.1452 mL | 0.2904 mL | 0.7261 mL |
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