Fast Green FCF
Based on 1 publication(s) in Google Scholar
Fast Green FCF is a sea green triarylmethane food dye, with absorption maximum ranging from 622 to 626 nm. Fast Green FCF inhibits α-synuclein aggregation, as well as Aβ and P2X4 receptor, and TLR4/Myd88/NF-κB. Fast Green FCF is widely used as a staining agent like quantitative stain for histones at alkaline pH after acid extraction of DNA, and as a protein stain in electrophoresis. Fast Green FCF improves cognitive impairment, depression, relieves pain allergies, and promotes reproductive function.
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- Purity: 99.95%
- CAS No.: 2353-45-9
- 화학식: C37H34N2Na2O10S3
- 분자량:808.85
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보관:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Fast Green FCF
MoreAll α-synuclein Isoforms
More
Biological Activity
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α-synuclein Aggregation |
Fast Green FCF (molar ratios of HEWL/FCF: 1 to 0.01-1 to 1, 10 days) suppresses the generation of lysozyme fibrils in a concentration-dependent manner and exhibits disaggregating activity against the preformed/existing lysozyme fibrils[2].
Fast Green FCF (100 μg/mL-1 mg/mL) inhibits synaptic activity in rat hippocampal interneurons, with half-maximum effective concentration of ~200 μg/mL (250 μM)[3].
Fast Green FCF (5-100 μM, 24 h) inhibits α-synuclein aggregation, disassembles mature fibrils and protects against amyloid-induced neurotoxicity in PC12 cells[4].
Fast green FCF (molar ratios of Aβ40 to FGF of 3:1-1:3) alleviates the cytotoxicity induced by Aβ40 fibrillogenesis in PC12 cells[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:PC12 cells with α-syn fibrils
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Concentration:5, 25, 50, 100 μM
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Incubation Time:24 h
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Result:Reduced the cytotoxicity, and the cell viability respectively reached 66.2% (5 μM) and 72.7% (25 μM).
Increased cell viability, reaching 76.4% (50 μM) and 89.0% (100 μM), respectively.
Fast Green FCF (10-100 mg/kg, i.p., once daily, 30 min before surgery) prevents postoperative cognitive dysfunction via the downregulation of the P2X4 receptor in mice[6].
Fast Green FCF (100 mg/kg, i.p., daily for 7 days) attenuates lipopolysaccharide-induced depressive-like behavior and downregulates TLR4/Myd88/NF-κB signal pathway in the mouse hippocampus[7].
Fast Green FCF (30-100 mg/kg, i.p., daily for 9 days) alleviates pain hypersensitivity and down-regulates the levels of spinal P2X4 expression and pro-inflammatory cytokines in a rodent inflammatory pain model[8].
Fast Green FCF (0.017-0.041 gm per kg body weight, p.o., 42 days) causes a significant decrease in testicular weight, tubular diameter, sperm density, and sperm motility[9].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Aβ-induced cognitive impairment mice[5]
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Dosage:30, 100 mg/kg
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Administration:Intraperitoneal injection (i.p.), 30 min prior to each trial
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Result:Did not alter the recognition index.
Prevented the Aβ42-induced recognition impairment at 100 mg/kg.
Prevented the Aβ42-induced impairment of spatial learning at 100 mg/kg.
reduced the Aβ42-induced memory impairment.
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Chemical Information
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CAS No. 2353-45-9
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Appearance Solid
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분자량 808.85
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화학식 C37H34N2Na2O10S3
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Color Dark green to black
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SMILES
O=S(C1=CC(C/[N+](CC)=C2C=C/C(C=C/2)=C(C3=CC=C(N(CC)CC4=CC=CC(S(=O)(O[Na])=O)=C4)C=C3)\C5=CC=C(O)C=C5S(=O)(O[Na])=O)=CC=C1)([O-])=O
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Synonyms
FD&C Green No. 3; Food green 3; C.I. 42053
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선적
Room temperature in continental US; may vary elsewhere.
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보관
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (1)
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Journal Impact Factor
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Most Recent
용액&용해도
H2O : 10 mg/mL (12.36 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
순도&문서
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Data Sheet (286 KB)
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SDS (644 KB)
- English - EN (644 KB)
- Français - FR (644 KB)
- Deutsch - DE (644 KB)
- Norwegian - NO (644 KB)
- Español - ES (644 KB)
- Swedish - SV (644 KB)
- Italian - IT (644 KB)
- Korean - KR (644 KB)
- Portuguese - PT (644 KB)
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Handling Instructions (2659 KB)
References
[1]. Mittal A, et al. Batch and bulk removal of a triarylmethane dye, Fast Green FCF, from wastewater by adsorption over waste materials. J Hazard Mater. 2009 Apr 30;163(2-3):568-77. [Content Brief]
[2]. How SC, et al. Examining the inhibitory potency of food additive fast green FCF against amyloid fibrillogenesis under acidic conditions. Food Funct. 2016 Dec 7;7(12):4898-4907. [Content Brief]
[3]. van Hooft JA. Fast Green FCF (Food Green 3) inhibits synaptic activity in rat hippocampal interneurons. Neurosci Lett. 2002 Feb 1;318(3):163-5. [Content Brief]
[4]. Wang F, et al. The food additive fast green FCF inhibits α-synuclein aggregation, disassembles mature fibrils and protects against amyloid-induced neurotoxicity. Food Funct. 2021 Jun 21;12(12):5465-5477. [Content Brief]
[5]. Liu F, et al. Fast green FCF inhibits Aβ fibrillogenesis, disintegrates mature fibrils, reduces the cytotoxicity, and attenuates Aβ-induced cognitive impairment in mice. Int J Biol Macromol. 2021 Feb 15;170:33-41. [Content Brief]
[6]. Liu J, et al. Fast green FCF prevents postoperative cognitive dysfunction via the downregulation of the P2X4 receptor in mice. Int Immunopharmacol. 2023 Aug;121:110462. [Content Brief]
[7]. Yang J, et al. Fast Green FCF Attenuates Lipopolysaccharide-Induced Depressive-Like Behavior and Downregulates TLR4/Myd88/NF-κB Signal Pathway in the Mouse Hippocampus. Front Pharmacol. 2019 May 8;10:501. [Content Brief]
[8]. Xu F, et al. Fast Green FCF Alleviates Pain Hypersensitivity and Down-Regulates the Levels of Spinal P2X4 Expression and Pro-inflammatory Cytokines in a Rodent Inflammatory Pain Model. Front Pharmacol. 2018 May 23;9:534. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.2363 mL | 6.1816 mL | 12.3632 mL | 30.9081 mL |
| 5 mM | 0.2473 mL | 1.2363 mL | 2.4726 mL | 6.1816 mL | |
| 10 mM | 0.1236 mL | 0.6182 mL | 1.2363 mL | 3.0908 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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