Medronic acid
Based on 1 publication(s) in Google Scholar
Medronic acid (Methylenediphosphonic acid) is a methylene-substituted bisphosphonate. Medronic acid has an affinity for the surface of hydroxyapatite crystals in the bone matrix and adheres to them. Medronic acid can be used in complex with radioisotopes in bone imaging. Due to its strong metal chelating ability, medronic acid is also used as a water treatment chemical. In addition, medronic acid is used as a solvent additive to improve peak shape and signal of metal-sensitive metabolites in LC/MS analysis.
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- Purity: 98.0%
- CAS No.: 1984-15-2
- 화학식: CH6O6P2
- 분자량:176.00
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보관:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Medronic acid
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Biological Activity
Preparation of standard solutions and spike solutions for the detection of intracellular purine and pyrimidine-related derivatives by liquid chromatography-tandem mass spectrometry[2]:
1. Prepare stock solutions of target analytes in 50% methanol at a concentration of 1 mg/mL for each stock solution.
2. To obtain a mixed working solution containing 31 analytes at a concentration of 10 μg/mL, dilute the stock solutions with 80% acetonitrile.
3. For internal standard preparation, prepare stock solutions of each standard in 50% methanol at a concentration of 0.5 mg/mL for each standard. An internal standard mixture containing 13C5-inosine, 13C5-cAMP, 13C10, 15N5-ATP, 13C9, 15N3-CTP, 13C10, 15N5-GTP, and 13C9, 15N2-UTP was diluted in 80% acetonitrile to give a final concentration of 50 ng/mL.
4. Prepare two additives, medronic acid and ammonium phosphate, with water, each at a concentration of 100 mM.
5. All solutions were stored at −20°C before use.
Detection of intracellular purine and pyrimidine-related derivatives by liquid chromatography-tandem mass spectrometry[2]:
1. Add 500 μL of methanol to the collected cell microspheres to be tested, sonicate for 5 min to lyse the cells, and incubate on ice for 5 min.
2. After protein precipitation, add 500 μL of water to the methanol extract, sonicate for 5 min, and incubate on ice for 5 min.
3. After extraction, centrifuge the sample at 15,000 rcf for 5 min at 4°C.
4. Collect all supernatant into another centrifuge tube and evaporate using a centrifugal evaporator.
5. Reconstitute the residue with 500 μL of 80% acetonitrile and filter through a 0.2 μm PP membrane filter.
6. After filtration, add 10 μL of internal standard mix and 10 μL of 5 mM medronic acid to 80 μL of sample solution to reach a final concentration of 0.5 mM medronic acid before analysis. In parallel, prepare a pooled QC sample to monitor system stability during analysis.
7. All samples were stored at −20 °C prior to LC-MS/MS analysis.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1984-15-2
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Appearance Solid
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분자량 176.00
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화학식 CH6O6P2
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Color White to off-white
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SMILES
O=P(O)(O)CP(O)(O)=O
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Synonyms
Methylenediphosphonic acid
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (1)
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Journal Impact Factor
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Most Recent
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Cell
2026 Mar 31:S0092-8674(26)00279-5. PMID: 41923640
용액&용해도
DMSO : 200 mg/mL (1136.36 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (11.82 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (11.82 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
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Data Sheet (278 KB)
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SDS (476 KB)
- English - EN (476 KB)
- Français - FR (476 KB)
- Deutsch - DE (476 KB)
- Norwegian - NO (476 KB)
- Español - ES (476 KB)
- Swedish - SV (476 KB)
- Italian - IT (476 KB)
- Korean - KR (476 KB)
- Portuguese - PT (476 KB)
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Handling Instructions (2659 KB)
References
[1]. Ohta Y, et al. Salt Tolerance Enhancement of Liquid Chromatography-Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry Using Matrix Additive MethylenediphosphonicAcid. Mass Spectrom (Tokyo). 2014;3(1):A0031. [Content Brief]
[2]. Lin Y T, et al. Using an In-Sample Addition of Medronic Acid for the Analysis of Purine-and Pyrimidine-Related Derivatives and Its Application in the Study of Lung Adenocarcinoma A549 Cell Lines by LC–MS/MS[J]. Journal of Proteome Research, 2023, 22(5): 1434-1445. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.6818 mL | 28.4091 mL | 56.8182 mL | 142.0455 mL |
| 5 mM | 1.1364 mL | 5.6818 mL | 11.3636 mL | 28.4091 mL | |
| 10 mM | 0.5682 mL | 2.8409 mL | 5.6818 mL | 14.2045 mL | |
| 15 mM | 0.3788 mL | 1.8939 mL | 3.7879 mL | 9.4697 mL | |
| 20 mM | 0.2841 mL | 1.4205 mL | 2.8409 mL | 7.1023 mL | |
| 25 mM | 0.2273 mL | 1.1364 mL | 2.2727 mL | 5.6818 mL | |
| 30 mM | 0.1894 mL | 0.9470 mL | 1.8939 mL | 4.7348 mL | |
| 40 mM | 0.1420 mL | 0.7102 mL | 1.4205 mL | 3.5511 mL | |
| 50 mM | 0.1136 mL | 0.5682 mL | 1.1364 mL | 2.8409 mL | |
| 60 mM | 0.0947 mL | 0.4735 mL | 0.9470 mL | 2.3674 mL | |
| 80 mM | 0.0710 mL | 0.3551 mL | 0.7102 mL | 1.7756 mL | |
| 100 mM | 0.0568 mL | 0.2841 mL | 0.5682 mL | 1.4205 mL |
Medronic acid Related Classifications
HY-108309 Related Classifications
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