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Cat. No.: HY-P991886

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Biologically Validated

ANX-M1 (Human IgG1)

Name: ANX-M1 (Human IgG1)
Brand: MedChemExpress
SKU: HY-P991886
Price: 300.00 USD
Availability: InStock
Rating: 5 (1 reviews)

고객리뷰

Based on 1 Customer Validation

ANX-M1 is a blood-brain barrier-permeable anti-C1q antibody. ANX-M1 can slow down the progression of retinal degeneration following photo-oxidative damage. ANX-M1 has been incorporated into nanocarriers to evaluate its brain delivery efficacy in a mouse model of Alzheimer's disease. ANX-M1 is applicable for research on age-related macular degeneration and Alzheimer's disease^[1].

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

Purity : 99.0%
분자량:145.78 kDa

보관:

Shipping with dry ice

Please store the product under the recommended conditions in the Certificate of Analysis.

ANX-M1 (Human IgG1) Chemical Structure

사이즈

가격

재고

수량

Amount: USD 0.00

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ANX-M1 (Human IgG1) Product Recommendations:

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- 제품 설명
- Isotype
- Recommend Isotype Controls
- Species Reactivity
- IC₅₀ & Target
- In Vivo
- Gene ID
- Accession
- Conjugated
- Reconsititution
- 신청
- Verified Bioactivity
Chemical Information
순도&문서
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

제품 설명

Isotype

Human IgG1 kappa

Recommend Isotype Controls

Human IgG1 kappa, Isotype Control

Species Reactivity

Human/Mouse

IC₅₀ & Target

C1q

In Vivo

ANX-M1 (7.5 μg/μL-100 mg/kg; intravitreal; single dose; intraperitoneal; days 0,4,8) protects against progressive retinal degeneration in C57BL/6J mice with photo-oxidative damage-induced retinal degeneration (with TUNEL⁺ photoreceptor cell death reduction, ONL thickness increase, ERG responses improvement, and serum complement hemolytic activity reduction of >50% for effective regimens)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

C57BL/6J (adult, postnatal day 60-90, equal numbers of male and female, photo-oxidative damage-induced retinal degeneration)^[1]
Dosage:

7.5 μg/μL (intravitreal post-treatment); 7.5 μg/μL (intravitreal pre-treatment); 100 mg/kg (systemic)
Administration:

intravitreal (single dose on day 7 or day 0); intraperitoneal (days 0,4,8)
Result:

Significantly reduced TUNEL+ photoreceptor cell death at day 14 compared to IgG control (P < 0.05), significantly increased outer nuclear layer (ONL) thickness (P < 0.05), significantly improved rod a-wave and b-wave electroretinography (ERG) responses (P < 0.05, most pronounced at 1.9 log cd.s/m²), and significantly improved cone ERG responses (P < 0.05) with intravitreal post-treatment; showed no significant differences in TUNEL+ cell count, ONL thickness, IBA1+ cell count, or ERG responses compared to IgG control (P > 0.05) with intravitreal pre-treatment; reduced serum complement hemolytic activity by >50% at day 12 (P < 0.05) but showed no significant differences in TUNEL+ cell count, ONL thickness, IBA1+ cell count, or ERG responses compared to IgG control (P > 0.05) with systemic administration

Related Protocols :

Gene ID

712 [NCBI]

Accession

P02745

Conjugated

Unconjugated

Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.

신청

ELISA, FACS, Functional assay

Verified Bioactivity

Immobilized Human C1Q Protein can bind ANX-M1 (Human IgG1). The ED₅₀ for this effect is 56.75 ng/mL.
Flow cytometric analysis of 1X10⁶ Hela cells labeling C1q with ANX-M1 (HY-P991886, red). Cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1 kappa (HY-P99001, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Chemical Information

Appearance Liquid
분자량 145.78 kDa
Color Colorless to light yellow
SMILES

[ANX-M1 (Human IgG1)]
선적

Shipping with dry ice.
Formulation

Please refer to the lot-specific COA for specific buffer information.
보관

Please store the product under the recommended conditions in the Certificate of Analysis.

순도&문서

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Inhibitory Antibodies User Guide (603 KB)

References

[1]. Jiao H, et al. Subretinal macrophages produce classical complement activator C1q leading to the progression of focal retinal degeneration. Mol Neurodegener. 2018;13(1):45. Published 2018 Aug 20. [Content Brief]

[2]. Gagnon M, et al. Evaluation of Novel B1R/B2R Agonists Containing TRIOZAN™ Nanoparticles for Targeted Brain Delivery of Antibodies in a Mouse Model of Alzheimer Disease. Molecules. 2023;28(13):5206. Published 2023 Jul 4. [Content Brief]

Calculators

몰농도 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

농도 희석 계산기

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

MOQ

Minimum order quantity

100 mg

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Other size

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Amount: USD 0.00

Biological Activity
- 제품 설명
- Isotype
- Recommend Isotype Controls
- Species Reactivity
- IC₅₀ & Target
- In Vivo
- Gene ID
- Accession
- Conjugated
- Reconsititution
- 신청
- Biological Activity
Chemical Information
순도&문서
References

1/16

Immobilized Human C1Q Protein can bind ANX-M1 (Human IgG1). The ED₅₀ for this effect is 56.75 ng/mL.
1/16

Flow cytometric analysis of 1X10⁶ Hela cells labeling C1q with ANX-M1 (HY-P991886, red). Cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1 kappa (HY-P99001, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
1/16

상품명:
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ANX-M1 (Human IgG1)