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Cat. No.: HY-P2893

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Creatinase, Actinobacteria

(Synonyms: Creatine amidohydrolase)

고객리뷰

Based on 1 Customer Validation

Creatinase, Actinobacteria (Creatine amidohydrolase) is a hydrolase that catalyzes the hydrolysis of creatine into sarcosine and urea, and plays an important role in the enzymatic determination of creatinine concentration.

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CAS No.: 37340-58-2

보관:

Please store the product under the recommended conditions in the Certificate of Analysis.

Creatinase, Actinobacteria Chemical Structure

CAS No.: 37340-58-2

사이즈

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재고

수량

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- 제품 설명
- In Vitro
- EC Number
- Enzyme Activity
- Unit Definition
Chemical Information
용액&용해도
- In Vitro
순도&문서
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

제품 설명

All Endogenous Metabolite Isoforms :

In Vitro

Product Information
This product can hydrolyze creatine and is used in the development and preparation of enzymatic creatinine reagents.
Molecular weight: 48 kDa (SDS-PAGE)
Isoelectric point: 4.6
Inhibitors: Ag ⁺, Cu ²⁺, Hg ²⁺
Optimum: pH 7.5
Optimum temperature: 45-50°C
pH stability 4.5-10.0 (25°C, 16 h)

Instructions
Can be dissolved in water (1 mg/mL), or according to specific experimental references (such as using 50 mM PBS, pH 7.5).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

EC Number

3.5.3.3

Enzyme Activity

≥10 U/mg soild

Unit Definition

One unit is defined as the amount of enzyme that can react creatine to produce 1 μmol sarcosine per minute at 37°C and pH 5.5.

Chemical Information

CAS No. 37340-58-2
Appearance Solid
Color White to off-white
SMILES

[Creatinase, Actinobacteria]
Synonyms

Creatine amidohydrolase
선적

Room temperature in continental US; may vary elsewhere.
보관

Please store the product under the recommended conditions in the Certificate of Analysis.

용액&용해도

In Vitro:

H₂O : 100 mg/mL (Need ultrasonic)

View the Complete Stock Solution Preparation Table

순도&문서

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (264 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. J Schumann, et al. Stabilization of creatinase from Pseudomonas putida by random mutagenesis. Protein Sci. 1993 Oct;2(10):1612-20. [Content Brief]

Calculators

몰농도 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

농도 희석 계산기

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

100 mg

견적 받기 해외재고보유

Amount: USD 0.00

Biological Activity
- 제품 설명
- In Vitro
- EC Number
- Enzyme Activity
- Unit Definition
Chemical Information
용액&용해도
- In Vitro
순도&문서
References

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Creatinase, Actinobacteria

All Endogenous Metabolite Isoforms