D-Dopa
Based on 1 Customer Validation
D-Dopa (3-Hydroxy-D-tyrosine) is a potent, orally active, non-competitive and allosteric inhibitor of glutamate carboxypeptidase II (GCPII) with an IC50 of 200 nM. D-Dopa elevates brain dopamine and produce turning behavior in rats. D-Dopa inhibit severe fever with thrombocytopenia syndrome (SFTS) virus infection. D-Dopa can be used for neurological disease and virus infection research.
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- Purity: 99.67%
- CAS No.: 5796-17-8
- 화학식: C9H11NO4
- 분자량:197.19
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보관:
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
Biological Activity
IC50: 200 nM (GCPII)
D-Dopa (10 µM, 0-60 min) exhibits good metabolic stability in mouse plasma (100% remaining) and mouse brain homogenate (82% remaining)[1].
D-Dopa (0-0.25 mM, 48 h) inhibits SFTS virus (SFTSV) in a dose-dependent manner mainly by blocking viral attachment to host cells, with IC50 values of 4.23 µM (by plaque assay) and 6.72 µM (by viral RNA quantification), respectively[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| Species | Dose | Route | Note | Cmax | Tmax | AUC0-t | Vd | CL | T1/2 | F |
|---|---|---|---|---|---|---|---|---|---|---|
| Mice[1] | 10 mg/kg | i.v. | / | 12048.309 ng/mL | 0.08 h | 6014.295 ng·h/mL | 834 mL/kg | 1678 mL/h/kg | 0.353 h | / |
| Mice[1] | 50 mg/kg | p.o. | plasma | 17739.81 ng/mL | 0.25 h | 13027.113 ng·h/mL | / | / | / | 47.7 % |
| Mice[1] | 50 mg/kg | p.o. | brain | 311.7906 ng/mL | 0.50 h | 433.6398 ng·h/mL | / | / | / | / |
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 5796-17-8
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Appearance Solid
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분자량 197.19
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화학식 C9H11NO4
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Color White to off-white
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Synonyms
3-Hydroxy-D-tyrosine
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선적
Room temperature in continental US; may vary elsewhere.
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보관
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
용액&용해도
1 M HCl : 50 mg/mL (253.56 mM; Need ultrasonic)
H2O : 14.29 mg/mL (72.47 mM; ultrasonic and adjust pH to 1 with 1 M HCl)
DMSO : 5 mg/mL (25.36 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 0.5 mg/mL (2.54 mM); Clear solution
This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 0.5 mg/mL (2.54 mM); Clear solution
This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
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Data Sheet (274 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Gori SS, et al., D-DOPA Is a Potent, Orally Bioavailable, Allosteric Inhibitor of Glutamate Carboxypeptidase II. Pharmaceutics. 2022 Sep 23;14(10):2018. [Content Brief]
[2]. Karoum F, et al. D-dopa and L-dopa similarly elevate brain dopamine and produce turning behavior in rats. Brain Res. 1988 Feb 2;440(1):190-4. [Content Brief]
[3]. Ogawa M, et al. L-DOPA, a treatment for Parkinson's disease, and its enantiomer D-DOPA inhibit severe fever with thrombocytopenia syndrome virus infection in vitro. J Infect Chemother. 2022 Mar;28(3):373-376. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O / 1 M HCl | 1 mM | 5.0713 mL | 25.3563 mL | 50.7125 mL | 126.7813 mL |
| 5 mM | 1.0143 mL | 5.0713 mL | 10.1425 mL | 25.3563 mL | |
| 10 mM | 0.5071 mL | 2.5356 mL | 5.0713 mL | 12.6781 mL | |
| 15 mM | 0.3381 mL | 1.6904 mL | 3.3808 mL | 8.4521 mL | |
| 20 mM | 0.2536 mL | 1.2678 mL | 2.5356 mL | 6.3391 mL | |
| 25 mM | 0.2029 mL | 1.0143 mL | 2.0285 mL | 5.0713 mL | |
| H2O / 1 M HCl | 30 mM | 0.1690 mL | 0.8452 mL | 1.6904 mL | 4.2260 mL |
| 40 mM | 0.1268 mL | 0.6339 mL | 1.2678 mL | 3.1695 mL | |
| 50 mM | 0.1014 mL | 0.5071 mL | 1.0143 mL | 2.5356 mL | |
| 60 mM | 0.0845 mL | 0.4226 mL | 0.8452 mL | 2.1130 mL | |
| 1 M HCl | 80 mM | 0.0634 mL | 0.3170 mL | 0.6339 mL | 1.5848 mL |
| 100 mM | 0.0507 mL | 0.2536 mL | 0.5071 mL | 1.2678 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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