Cat. No.: HY-140272

DBCO-PEG4-NHS ester

Name: DBCO-PEG4-NHS ester
Brand: MedChemExpress
SKU: HY-140272
Price: 40.00 USD
Availability: InStock
Rating: 5 (4 reviews)

4 Cited Publications

고객리뷰

Based on 4 publication(s) in Google Scholar

DBCO-PEG4-NHS ester is a PEG-based PROTAC linker that can be used in the synthesis of PROTACs. DBCO-PEG4-NHS ester is a click chemistry reagent, it contains a DBCO group that can undergo strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing Azide groups.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

Purity : 97.13%
CAS No.: 1427004-19-0
화학식: C₃₄H₃₉N₃O₁₀
분자량:649.69

보관:

Please store the product under the recommended conditions in the Certificate of Analysis.

DBCO-PEG4-NHS ester Chemical Structure

CAS No.: 1427004-19-0

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- 제품 설명
- IC₅₀ & Target
- In Vitro
Chemical Information
Publications (4)
용액&용해도
- In Vitro
- In Vivo
Protocol (1)
순도&문서
References
Help & FAQs
Classifications
Bioz

Biological Activity

제품 설명

DBCO-PEG4-NHS ester is a PEG-based PROTAC linker that can be used in the synthesis of PROTACs^[1]. DBCO-PEG4-NHS ester is a click chemistry reagent, it contains a DBCO group that can undergo strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing Azide groups.

IC₅₀ & Target

PEGs

All PROTAC Linkers Isoforms :

In Vitro

PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.

Chemical Information

CAS No. 1427004-19-0
Appearance Solid-Liquid Mixture
분자량 649.69
화학식 C₃₄H₃₉N₃O₁₀
Color Light yellow to brown
SMILES

O=C(ON1C(CCC1=O)=O)CCOCCOCCOCCOCCNC(CCC(N2C3=CC=CC=C3C#CC4=CC=CC=C4C2)=O)=O
선적

Shipping with dry ice.
보관

Please store the product under the recommended conditions in the Certificate of Analysis.

Publications (4)

All Bio/Physico-chemical AssayOthersCell Proliferation/Viability Assay

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Journal Impact Factor

Adv Mater

A Bio-Orthogonal Engineered Chitosan Platform for Enhanced Mesenchymal Stem Cells Delivery and Function in Peripheral Nerve Repair. [Abstract]

2026 Mar;38(13):e23237. PMID: 41608909

DBCO-PEG4-NHS ester purchased from MedChemExpress. Usage Cited in: Adv Mater. 2026 Mar;38(13):e23237. [Abstract]

FTIR of DBCO–Chitosan prepared with varying doses of DBCO-PEG4-NHS ester.

DBCO-PEG4-NHS ester purchased from MedChemExpress. Usage Cited in: Adv Mater. 2026 Mar;38(13):e23237. [Abstract]

Representative fluorescence imaging of DBCO-PEG4-NHS ester-modified chitosan membranes incubated with N3–PEG–FITC.

DBCO-PEG4-NHS ester purchased from MedChemExpress. Usage Cited in: Adv Mater. 2026 Mar;38(13):e23237. [Abstract]

Representative stress–strain curves of chitosan membranes with/without DBCO-modified.

DBCO-PEG4-NHS ester purchased from MedChemExpress. Usage Cited in: Adv Mater. 2026 Mar;38(13):e23237. [Abstract]

Scanning electron microscopy (SEM) image of DBCO–Chitosan membranes. Scale bar, 500, and 100 µm (magnification).

DBCO-PEG4-NHS ester purchased from MedChemExpress. Usage Cited in: Adv Mater. 2026 Mar;38(13):e23237. [Abstract]

A live/dead staining assay revealeda decrease in fibroblast viability when the DBCO-PEG4 -NHSester concentration reached 2.5 µm.Cellular toxicity of chitosan materials before and after DBCO modification. a) Live/dead staining of chitosan membranes with or without DBCO modification with Calcein-AM (1:1000) and PI (1:800) staining at 48 and 72 h. Scale bar, 50 μm. b) The cell viability of L929 cells in each group at 48 h (n = 3). Data are expressed as mean ± SD. ****p < 0.001, and ns represent p﹥0.05. Data were analyzed by Two-tailed Student’s t-tests for pairwise group comparisons.
Adv Sci (Weinh)

Functionalized Biomimetic Nanoparticles Targeting the IL-10/IL-10Rα/Glycolytic Axis in Synovial Macrophages Alleviate Cartilage Degeneration in Osteoarthritis. [Abstract]

2025 Jul;12(27):e2504768. PMID: 40317692
Int J Biol Macromol

Inhibition of ESCRT-independent extracellular vesicles biogenesis suppresses enterovirus 71 replication and pathogenesis in mice. [Abstract]

2024 Apr 6;267(Pt 1):131453. PMID: 38588842
Chem Eng J Adv

2026 Feb 16.

용액&용해도

In Vitro:

DMSO : 70 mg/mL (107.74 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.5392 mL

7.6960 mL

15.3920 mL

5 mM

0.3078 mL

1.5392 mL

3.0784 mL

10 mM

0.1539 mL

0.7696 mL

1.5392 mL

몰농도 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

농도 희석 계산기

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (3.20 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: 2.08 mg/mL (3.20 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (3.20 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Protocol

All Cell Biology

EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.

순도&문서

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (270 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. An S, et al. Small-molecule PROTACs: An emerging and promising approach for the development of targeted therapy drugs. EBioMedicine. 2018 Oct;36:553-562 [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.5392 mL	7.6960 mL	15.3920 mL	38.4799 mL
	5 mM	0.3078 mL	1.5392 mL	3.0784 mL	7.6960 mL
	10 mM	0.1539 mL	0.7696 mL	1.5392 mL	3.8480 mL
	15 mM	0.1026 mL	0.5131 mL	1.0261 mL	2.5653 mL
	20 mM	0.0770 mL	0.3848 mL	0.7696 mL	1.9240 mL
	25 mM	0.0616 mL	0.3078 mL	0.6157 mL	1.5392 mL
	30 mM	0.0513 mL	0.2565 mL	0.5131 mL	1.2827 mL
	40 mM	0.0385 mL	0.1924 mL	0.3848 mL	0.9620 mL
	50 mM	0.0308 mL	0.1539 mL	0.3078 mL	0.7696 mL
	60 mM	0.0257 mL	0.1283 mL	0.2565 mL	0.6413 mL
	80 mM	0.0192 mL	0.0962 mL	0.1924 mL	0.4810 mL
	100 mM	0.0154 mL	0.0770 mL	0.1539 mL	0.3848 mL

Bioz

Keywords

DBCO-PEG4-NHS ester

4 Cited Publications

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DBCO-PEG4-NHS ester

Publications Citing Use of MedChemExpress (MCE) DBCO-PEG4-NHS ester

All PROTAC Linkers Isoforms