DFHBI-1T (solution)

Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Preparation of stock solution^[1]
It is recommended that the DFHBI-1T stock solution be stored at -20°C or -80°C in the dark after aliquoting.
2. Preparation of working solution
Dilute the stock solution with serum-free medium. The corresponding stock solution can be diluted according to the actual situation. Note that if the solvent is DMSO, the cytotoxicity of DMSO must be considered, and a solvent control should be prepared; if the solvent is pure water, the working solution needs to be filtered and sterilized before adding cells.
Note: Please adjust the concentration of DFHBI-1T working solution according to actual conditions and prepare it before use.
3. Specific staining steps
3.1 Inoculate cells expressing Spinach2-tagged RNA in a suitable culture dish or culture plate and culture under standard cell culture conditions.
3.2 Aspirate the old medium and gently wash the cells twice with pre-warmed PBS to remove residual serum and cell debris.
3.3 Add 20 μM DFHBI-1T working solution to the cells, ensuring complete coverage of the cell monolayer.
3.4 Incubate the cells with the dye for 10 min in a 37°C, 5% CO₂ humidified incubator.
3.5 After incubation, aspirate the dye-containing medium and wash the cells three times with pre-warmed PBS to remove unbound DFHBI-1T.
3.6 Replace PBS with fresh cell culture medium or imaging buffer.
3.7 Immediately image the cells using a fluorescence microscope equipped with a GFP filter set to detect fluorescence of the Spinach2-DFHBI-1T complex.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.