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EGTA

27 Cited Publications
고객리뷰

Based on 27 publication(s) in Google Scholar

EGTA is a cell-impermeant specific calcium ion chelator. EGTA has an apparent calcium dissociation constant (Kd) of 60.5 nM at physiological pH (7.4) and has very high specificity for Ca2+ over Mg2+ (Mg2+ Kd 1-10 mM). EGTA significantly inhibits the substrate adherence capacity of inflammatory macrophages.

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  • Purity: 99.89%
  • CAS No.: 67-42-5
  • 화학식: C14H24N2O10
  • 분자량:380.35
  • 보관:

    Store at room temperature 3 years.

    In solvent   -80°C, 2 years , -20°C, 1 year
  • EGTA Chemical Structure
    EGTA Chemical Structure
  • Experimental Validation Results for EGTA
  • Experimental Validation Results for EGTA
  • Experimental Validation Results for EGTA
  • Experimental Validation Results for EGTA
  • Experimental Validation Results for EGTA
  • Experimental Validation Results for EGTA
  • Experimental Validation Results for EGTA
  • Experimental Validation Results for EGTA
CAS No.: 67-42-5
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Publications Citing Use of MedChemExpress (MCE) EGTA

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Flow CytometryBio/Physico-chemical AssayIF2D/3D Cell Culture and DifferentiationWBCell Autophagy AssayCell Proliferation/Viability AssayCell Imaging/Staining
  • 2D/3D Cell Culture and Differentiation
  • WB
  • Cell Proliferation/Viability Assay
  • Cell Imaging/Staining
  • Flow Cytometry
  • Bio/Physico-chemical Assay
  • IF
  • Cell Autophagy Assay
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  • EGTA Chemical Structure
    EGTA Chemical Structure
    1/16
  • 1/16

    EGTA purchased from MedChemExpress. Usage Cited in: mBio. 2024 Jun 3:e0119124. [Abstract]

    Customer Validation
     HFFs were infected with Towne or Towne-ΔUL23 and co-cultured with or without activated Jurkat cells, followed by determination of viral titers. Prior to co-culture, the culture medium was sequentially supplemented with EGTA (5 mg/mL), Kp7-6 (10 mg/mL), anti-human IFN-γ neutralizing antibody (IFN-γ NAb, 10 µg/mL), anti-TRAIL neutralizing antibody (TRAIL NAb, 10 µg/mL), and infliximab (10 ng/mL) for 30 min.

  • 1/16

    EGTA purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2024 Feb 15:128:111537. [Abstract]

    Customer Validation
     EGTA (30 nM; 2 h). NLRP3/caspase-1/GSDMD protein levels (D) in BMDM cell by LPS + ATP.

  • 1/16

    EGTA purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2024 Feb 15:128:111537. [Abstract]

    Customer Validation
     Cell viability in BMDM cell by LPS + ATP. The results showed that EGTA (30 nM; 2 h) promoted cell viability.

  • 1/16

    EGTA purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2024 Feb 15:128:111537. [Abstract]

    Customer Validation
     Mitochondrion (Electron microscope) in BMDM cell by LPS + ATP. The results showed that EGTA (30 nM; 2 h) inhibited mitochondrial damage.

  • 1/16

    EGTA purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2023 Nov 28:577:216435. [Abstract]

    Customer Validation
     Flow cytometric analysis of Annexin V-FITC/PI-PerCP-stained cell line Hut-102 were pretreated with CDN-1163 (50 μM), 2-APB (100 μM) or EGTA (5 mM) for 1 h, then exposed to 15 μM LW-213 for 6 h (n ≥ 3, ns means no significant difference, *p < 0.05, **p < 0.01 vs. 15 μM).

  • 1/16

    EGTA purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2023 Jun 4;19(9):2914-2933. [Abstract]

    Customer Validation
     Ca2+ mobilization in FLAG-LcMtnr1b1-expressing HEK293 cells were measured in response to indicated concentrations of MT with pre-treated intracellular calcium chelator (BAPTA-AM, 50 μM), extracellular calcium chelator (EGTA, 5 mM; 30-300 min), or BAPTA-AM+EGTA, respectively.

  • 1/16

    EGTA purchased from MedChemExpress. Usage Cited in: J Anim Sci Biotechnol. 2023 Dec 20;14(1):157. [Abstract]

    Customer Validation
     MMEC was pretreated with calcium chelating agent EGTA (3 mM), and then added with S. maltophilia for 6 h before being collected. Calcium concentration was measured by Flou-4 fluorescence probe.

  • 1/16

    EGTA purchased from MedChemExpress. Usage Cited in: J Anim Sci Biotechnol. 2023 Dec 20;14(1):157. [Abstract]

    Customer Validation
     MMEC was pretreated with calcium chelating agent EGTA (3 mM), and then added with S. maltophilia for 6 h before being collected. The autophagic level of MMECs was significantly reduced by EGTA pretreatment.

  • EGTA