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Cat. No.: HY-D3186

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HMRef-βGal

Name: HMRef-βGal
Brand: MedChemExpress
SKU: HY-D3186
Availability: PreOrder

HMRef-βGal is a fluorescent probe and a substrate responsive to β-galactosidase (β-galactosidase) (Ex/Em=498 nm/505-600 nm). After being cleaved by β-galactosidase, HMRef-βGal triggers significant fluorescence enhancement via intramolecular spirocyclic function regulation. HMRef-βGal generates bright fluorescence in cancer cells with elevated β-galactosidase activity, enabling visualization of tiny peritoneal metastases in mouse models. HMRef-βGal exhibits low in vitro cytotoxicity and low acute in vivo toxicity in mice. HMRef-βGal can be used for preclinical fluorescence-guided diagnosis and cytoreductive surgery of peritoneal metastases, including compatibility with real-time naked-eye detection and endoscopic imaging, as well as for studies related to peritoneal metastases of ovarian cancer.

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CAS No.: 2163840-29-5
화학식: C₂₈H₂₆F₃NO₈
분자량:561.50

보관:

Please store the product under the recommended conditions in the Certificate of Analysis.

HMRef-βGal Chemical Structure

CAS No.: 2163840-29-5

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- 제품 설명
- In Vitro
- In Vivo
Chemical Information
순도&문서
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

제품 설명

In Vitro

HMRef-βGal (10 μM; 1 h) sensitively detects intracellular β-galactosidase activity in SHIN3, SKOV3, OVK18, OVCAR3, OVCAR4, OVCAR5, and OVCAR8 human ovarian cancer cell lines via live-cell confocal imaging (Ex/Em=498 nm/505-600 nm)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

HMRef-βGal (100 μM, 300 μL; intraperitoneal injection; single dose; imaging performed 5 min or 1 h post-administration) enables sensitive and specific visual imaging of tiny peritoneal ovarian cancer metastases with a diameter <1 mm in 7 distinct mouse models, and its signal brightness is sufficient to support naked-eye and endoscopic detection (imaging with a blue-green filter, Ex=470-510 nm; Em=530 nm)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

BALB/cAJcl-nu/nu nude mice with Ovarian cancer (female, 7-8 weeks old)^[1]
Dosage:

300 μL of 100 μM
Administration:

i.p.; single dose
Result:

Specifically visualized peritoneal metastases as small as <1 mm in diameter in all 7 ovarian cancer cell line models at 5 min post-administration.
Enhanced tumor nodule fluorescence sufficiently for naked-eye detection at 1 h post-administration.
Suppressed tumor nodule fluorescence when co-treated with a β-galactosidase inhibitor.
Successfully visualized metastases via real-time fluorescence endoscopy.

Chemical Information

CAS No. 2163840-29-5
분자량 561.50
화학식 C₂₈H₂₆F₃NO₈
SMILES

FC(F)(F)CNC1=CC=C2C3(C4=CC=CC=C4CO3)C5=CC=C(C=C5OC2=C1)O[C@@H]6O[C@@H]([C@@H]([C@@H]([C@H]6O)O)O)CO
선적

Room temperature in continental US; may vary elsewhere.
보관

Please store the product under the recommended conditions in the Certificate of Analysis.

순도&문서

Handling Instructions (2659 KB)

References

[1]. Asanuma D, et al. Sensitive β-galactosidase-targeting fluorescence probe for visualizing small peritoneal metastatic tumours in vivo. Nat Commun. 2015;6:6463. Published 2015 Mar 13. [Content Brief]

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

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MOQ

Minimum order quantity

100 mg

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