Hydroxy-PEG3-DBCO
Hydroxy-PEG3-DBCO is a click chemistry reagent containing an azide group. Hydroxy-PEG3-DBCO is a PEG linker containing a DBCO moiety and a terminal primary hydroxyl group. The hydroxyl can react with a variety of functional groups and the hydrophilic PEG spacer arm can provide better solubility to labeled molecules. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research use only.
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- CAS No.: 2566404-76-8
- 화학식: C27H32N2O6
- 분자량:480.55
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보관:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocol
- 순도&문서
- References
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Help & FAQs
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HY-140272
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HY-120770
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HY-140279
DBCO-PEG4-alcoholPROTAC Linker -
HY-126885
DBCO-PEG5-NHS esterADC/PROTAC Linker -
HY-130396
DBCO-PEG4-C2-acidPROTAC Linker -
HY-140274
DBCO-PEG12-NHS esterPROTAC Linker -
HY-134714
DBCO-PEG3-aminePROTAC Linker -
HY-D1070
DBCO-PEG4-TAMRATARMA Dye -
HY-140280
DBCO-PEG1PROTAC Linker -
HY-140301
DBCO-PEG4-DesthiobiotinPROTAC Linker
Biological Activity
제품 설명
Chemical Information
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CAS No. 2566404-76-8
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분자량 480.55
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화학식 C27H32N2O6
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SMILES
O=C(CCC(N1C2=C(C#CC3=C(C1)C=CC=C3)C=CC=C2)=O)NCCOCCOCCOCCO
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocol
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
순도&문서
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Hydroxy-PEG3-DBCO Related Classifications
HY-151822 Related Classifications
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