L-Ethionine
Based on 1 Customer Validation
L-Ethionine (L-ETH) is an amino acid derivative. L-Ethionine serves as a substrate for ATP:L-methionine S-adenosyltransferase, which targets its L-stereoisomer. L-Ethionine blocks the synthesis of N6-dimethyladenosine in rat liver tRNA/4S RNA via its major hepatic metabolite S-adenosylethionine, inducing methyl-deficient rat liver tRNA/4S RNA. L-Ethionine can be used in studies of liver cancer and hepatocarcinogenesis mechanisms.
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- Purity: 99.66%
- CAS No.: 13073-35-3
- 화학식: C6H13NO2S
- 분자량:163.24
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보관:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
Biological Activity
L-Ethionine (L-ETH) (10 mM; 24-48 h) elevates AFP mRNA levels up to eightfold and albumin mRNA levels up to threefold in primary Balb/cJ mouse hepatocyte cultures after 48 hours of treatment with 10 mM L-ethionine[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
L-Ethionine (10 mg; i.p.; single injection) induces a 10- to 30-fold greater increase in hepatic α-fetoprotein mRNA levels in Balb/cJ mice compared to Balb/cCRBL, C3H/He, and C57BL/6 mice, while leaving hepatic albumin mRNA levels unaffected[3].
L-Ethionine (10-2500 mg/kg; i.p.; single dose) exhibits very low acute toxicity in male Swiss mice, with no mortality observed at doses up to 2500 mg/kg and only 1 death at 640 mg/kg over a 7-day observation period[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Swiss mice (male, ≤14 weeks, 27-41 grams, fasted)[1]
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Dosage:10 mg/kg; 20 mg/kg; 40 mg/kg; 100 mg/kg; 200 mg/kg; 320 mg/kg; 640 mg/kg; 1250 mg/kg; 2500 mg/kg
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Administration:i.p.; single dose
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Result:Showed no deaths in treated mice at doses of 10, 20, 40, 100, 200, 320, 1250, and 2500 mg/kg.
Caused 1 out of 6 treated mice to die at 640 mg/kg.
Resulted in all deaths occurring within 72 hours of administration.
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Animal Model:Wistar rats (female)[2]
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Dosage:0.3% (dietary regimens); 25 μCi (i.p. single dose)
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Administration:dietary; daily; 4 weeks-3 months; dietary; daily; 4 weeks-3 months followed by 10 days normal diet; i.p.; single dose
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Result:Completely eliminated N6-dimethyladenosine from rat liver 4S RNA after 4 weeks or 3 months of dietary exposure.
Maintained absence of N6-dimethyladenosine in rat liver 4S RNA after 10 days of normal diet post dietary exposure.
Resulted in eight ethylated nucleotides in liver 4S RNA after single i.p. injection.
Produced no visible ethylated nucleotides in liver 4S RNA from rats fed dietary L-ethionine for 4 weeks or 3 months.
Caused weak ethylation of 4S RNA compared to strong, selective inhibition of N6-dimethyladenosine synthesis.
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Animal Model:Balb/cJ, Balb/cCRBL, C3H/He, C57BL/6 (female, 7-10 weeks old, fasted 18-42 h prior to reagent injection)[3]
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Dosage:10 mg
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Administration:i.p.; single injection
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Result:Caused significant triglyceride accumulation in hepatic parenchymal cells and increased hepatic α-fetoprotein mRNA levels 48-96 h post-injection.
Increased hepatic α-fetoprotein mRNA levels in Balb/cJ mice 10- to 30-fold higher than in Balb/cCRBL, C3H/He, or C57BL/6 mice.
Reached peak α-fetoprotein mRNA levels 48-72 h post-injection in all strains, with levels returning to normal by day 8.
Left hepatic albumin mRNA levels unchanged across all strains during acute ethionine poisoning.
Elevated serum α-fetoprotein levels 72-96 h post-injection, with levels in Balb/cJ mice higher than in Balb/cCRBL mice.
Chemical Information
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CAS No. 13073-35-3
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Appearance Solid
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분자량 163.24
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화학식 C6H13NO2S
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Color Light yellow to yellow
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Synonyms
L-ETH
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선적
Room temperature in continental US; may vary elsewhere.
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보관
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
용액&용해도
H2O : ≥ 25 mg/mL (153.15 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
순도&문서
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Data Sheet (281 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 6.1259 mL | 30.6297 mL | 61.2595 mL | 153.1487 mL |
| 5 mM | 1.2252 mL | 6.1259 mL | 12.2519 mL | 30.6297 mL | |
| 10 mM | 0.6126 mL | 3.0630 mL | 6.1259 mL | 15.3149 mL | |
| 15 mM | 0.4084 mL | 2.0420 mL | 4.0840 mL | 10.2099 mL | |
| 20 mM | 0.3063 mL | 1.5315 mL | 3.0630 mL | 7.6574 mL | |
| 25 mM | 0.2450 mL | 1.2252 mL | 2.4504 mL | 6.1259 mL | |
| 30 mM | 0.2042 mL | 1.0210 mL | 2.0420 mL | 5.1050 mL | |
| 40 mM | 0.1531 mL | 0.7657 mL | 1.5315 mL | 3.8287 mL | |
| 50 mM | 0.1225 mL | 0.6126 mL | 1.2252 mL | 3.0630 mL | |
| 60 mM | 0.1021 mL | 0.5105 mL | 1.0210 mL | 2.5525 mL | |
| 80 mM | 0.0766 mL | 0.3829 mL | 0.7657 mL | 1.9144 mL | |
| 100 mM | 0.0613 mL | 0.3063 mL | 0.6126 mL | 1.5315 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
L-Ethionine Related Classifications
HY-W017200 Related Classifications
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