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Cat. No.: HY-183876

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LAF-153

LAF-153 is a Methionine Aminopeptidase-2 (MetAP-2) inhibitor with human IC₅₀ values of 0.074 μM and 0.16 μM.LAF-153 has a t-butyl group extending into the innermost portion of the MetAP-2 cleft, a lactam ring coordinated in the enzyme’s shallow surface pocket, and three hydroxyl groups coordinating to the two cobalt ions in the enzyme’s active site.

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CAS No.: 661482-39-9
화학식: C₁₈H₃₂N₂O₇
분자량:388.46

보관:

Please store the product under the recommended conditions in the Certificate of Analysis.

LAF-153 Chemical Structure

CAS No.: 661482-39-9

Amount: USD 0.00

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- 제품 설명
- Cellular Effect
- In Vitro
Chemical Information
Protocol (1)
순도&문서
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

제품 설명

All Aminopeptidase Isoforms :

CD13
LTA4H

Cellular Effect

Cell Line	Type	Value	Description	References
HeLa	IC₅₀	150 μM Compound: 9c	Cytotoxicity in human HeLa cells assessed as reduction in cell growth incubated for 72 hrs by neutral red staining based colorimetry Cytotoxicity in human HeLa cells assessed as reduction in cell growth incubated for 72 hrs by neutral red staining based colorimetry	[PMID: 30108762]
HepG2	IC₅₀	150 μM Compound: 9c	Cytotoxicity in human HepG2 cells assessed as reduction in cell growth incubated for 72 hrs by neutral red staining based colorimetry Cytotoxicity in human HepG2 cells assessed as reduction in cell growth incubated for 72 hrs by neutral red staining based colorimetry	[PMID: 30108762]
HL-60	IC₅₀	150 μM Compound: 9c	Cytotoxicity in human HL60 cells assessed as reduction in cell growth incubated for 72 hrs by neutral red staining based colorimetry Cytotoxicity in human HL60 cells assessed as reduction in cell growth incubated for 72 hrs by neutral red staining based colorimetry	[PMID: 30108762]
KB	IC₅₀	62.6 μM Compound: 9c	Cytotoxicity in human KB cells assessed as reduction in cell growth incubated for 72 hrs by neutral red staining based colorimetry Cytotoxicity in human KB cells assessed as reduction in cell growth incubated for 72 hrs by neutral red staining based colorimetry	[PMID: 30108762]
Lu1	IC₅₀	23.4 μM Compound: 9c	Cytotoxicity in human Lu1 cells assessed as reduction in cell growth incubated for 72 hrs by neutral red staining based colorimetry Cytotoxicity in human Lu1 cells assessed as reduction in cell growth incubated for 72 hrs by neutral red staining based colorimetry	[PMID: 30108762]
MCF7	IC₅₀	23.4 μM Compound: 9c	Cytotoxicity in human MCF7 cells assessed as reduction in cell growth incubated for 72 hrs by neutral red staining based colorimetry Cytotoxicity in human MCF7 cells assessed as reduction in cell growth incubated for 72 hrs by neutral red staining based colorimetry	[PMID: 30108762]

In Vitro

LAF-153 reversibly binds to human MetAP-2 via coordination of its hydroxyl groups to the enzyme's cobalt ions, with its t-butyl group and lactam ring occupying distinct pockets in the enzyme cleft^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.

Chemical Information

CAS No. 661482-39-9
분자량 388.46
화학식 C₁₈H₃₂N₂O₇
SMILES

CC(C)(C)/C=C/[C@@H](O)[C@H](O)[C@@H](O)[C@@H](OC)C(N[C@@H]1C(NC[C@@H](CC1)O)=O)=O
선적

Room temperature in continental US; may vary elsewhere.
보관

Please store the product under the recommended conditions in the Certificate of Analysis.

Protocol

All Cell Biology

Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.

순도&문서

Handling Instructions (2659 KB)

References

[1]. Heinrich T, et al. Discovery and structure-based optimization of next-generation reversible methionine aminopeptidase-2 (MetAP-2) inhibitors[J]. Journal of medicinal chemistry, 2019, 62(10): 5025-5039. [Content Brief]

Calculators

몰농도 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

농도 희석 계산기

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

Keywords

MOQ

Minimum order quantity

100 mg

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