MS6178
MS6178 is a MS5105-based CFTR deubiquitinase-targeted chimera (DUBTAC) that recruits OTUB1 to stabilize ΔF508-CFTR mutant protein in a concentration- and time-dependent manner, with an OTUB1-dependent effect and no impact on CFTR mRNA level. MS6178 can be used for the research of cancer.
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- CAS No.: 3060520-53-5
- 화학식: C47H53F2N7O7S
- 분자량:898.03
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보관:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocol
- 순도&문서
- References
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Help & FAQs
Biological Activity
제품 설명
In Vitro
MS6178 (0.5-10 µM; 0-24 h) effectively stabilizes the ΔF508-CFTR mutant protein in CFBE41o-4.7 ΔF508-CFTR cells in a concentration-, time-, OTUB1-, and CFTR-dependent manner (30-fold increase at 10 μM for 24 h)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 3060520-53-5
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분자량 898.03
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화학식 C47H53F2N7O7S
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SMILES
O=C(NC1=NC(C2=CC=CC(C(NCCCCCCC(NCCC3=CC=C(N4CCN(C(/C=C/CN(C)C)=O)CC4=O)S3)=O)=O)=C2)=C(C)C=C1)C5(CC5)C6=CC7=C(OC(F)(F)O7)C=C6
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocol
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RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
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Real Time qPCR (Q-PCR)
Real-time quantitative PCR (qPCR) quantifies an amplifiable nucleic-acid target by monitoring fluorescence during PCR cycling rather than measuring product only after amplification. The increase in fluorescence tracks accumulation of PCR product, and the quantification cycle (Cq; historically also Ct/CP) is related to the initial amount of target: samples containing more starting target generally reach the defined fluorescence threshold in fewer cycles.
순도&문서
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
MS6178 Related Classifications
HY-185122 Related Classifications
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