Myramistin
Based on 1 Customer Validation
Myramistin (Miramistin) is a cationic surfactant and disinfectant preservative. Myramistin exhibits high bactericidal activity against Staphylococcus aureus and Escherichia coli, shows lower activity against yeasts and fungi, and is active against HIV, measles virus, mumps virus, as well as influenza viruses of the H3N2 and H5N1 subtypes. Myramistin adsorbs to the negatively charged microbial cell membrane, leading to increased membrane permeability and disruption of membrane integrity, which ultimately results in leakage of intracellular contents, enzyme inactivation, and the death of bacteria/fungi/enveloped viruses.
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- Purity : 98.32%
- CAS No.: 15809-19-5
- 화학식: C26H47ClN2O
- 분자량:439.12
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보관:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- Protocol
- 순도&문서
- References
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Help & FAQs
Biological Activity
제품 설명
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Fibroblast | CC50 |
7.21 μg/mL
Compound: Miramistin
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Cytotoxicity against human skin fibroblasts assessed as cell viability measured after 72 hrs by MTT assay
Cytotoxicity against human skin fibroblasts assessed as cell viability measured after 72 hrs by MTT assay
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[PMID: 33385851] |
| HEK293 | CC50 |
6.08 μg/mL
Compound: Miramistin
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Cytotoxicity against human HEK293 cells assessed as cell viability measured after 72 hrs by MTT assay
Cytotoxicity against human HEK293 cells assessed as cell viability measured after 72 hrs by MTT assay
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[PMID: 33385851] |
| HSF (VGS) | CC50 |
9.4 μg/mL
Compound: Miramistin
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Cytotoxicity against human HSF cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
Cytotoxicity against human HSF cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
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[PMID: 33310546] |
In Vitro
Myramistin (0.1-0.2%; 1-15 min) reduces the log10 reduction coefficient of Staphylococcus aureus (ATCC 209p) on metal surfaces by ≥ 3.20 and that of Escherichia coli (CDC F-50) by ≥ 3.41 after 1 minute of exposure; its activity is reduced by 1-3 log10 units compared with that in suspension tests[4].
Myramistin (0.5-1000 μg/mL; 24 h) potently inhibits and kills Staphylococcus aureus (ATCC 209p), with an MIC of 8 μg/mL and an MBC of 16 μg/mL; it also inhibits and kills Escherichia coli (CDC F-50), with an MIC of 32 μg/mL and an MBC of 128 μg/mL[4].
Myramistin (5-10 μg/mL; 17-24 h) inhibits the growth of Staphylococcus aureus FDA 209P, with an MIC of 5 μg/mL[2].
Myramistin (30-50 μg/mL) exhibits dose-dependent anti-HIV activity in MT-4 cells, and inhibits HIV p24 production in Jurkat-tat cells when added simultaneously with HIV-1[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Sprague-Dawley (male, 200-230 g, experimentally induced bacterial skin infection)[4]
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Dosage:0.2%
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Administration:topical; single exposure; 5 minutes
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Result:Reduced the number of viable E. coli cells by approximately 2.6 log10 units.
Clinical Trial
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 15809-19-5
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Appearance Solid
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분자량 439.12
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화학식 C26H47ClN2O
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Color Light yellow to yellow
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SMILES
CCCCCCCCCCCCCC(NCCC[N+](C)(C)CC1=CC=CC=C1)=O.[Cl-]
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Synonyms
Miramistin
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선적
Room temperature in continental US; may vary elsewhere.
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보관
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
용액&용해도
In Vitro:
DMSO : 100 mg/mL (227.73 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 4 mg/mL (9.11 mM); Clear solution
This protocol yields a clear solution of ≥ 4 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (40.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 4 mg/mL (9.11 mM); Clear solution
This protocol yields a clear solution of ≥ 4 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (40.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
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Data Sheet (275 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Kryvorutchenko IuL, et al. Antiseptic myramistin possesses the strong anti-HIV properties. Mikrobiolohichnyi zhurnal (Kiev, Ukraine : 1993). 1996;58(5):90-2. [Content Brief]
[2]. Vertelov GK, et al. A versatile synthesis of highly bactericidal Myramistin® stabilized silver nanoparticles. Nanotechnology. 2008 Sep 03;19(35):355707. [Content Brief]
[3].
Agafonov AP, et al. In vitro study of antiviral activity of Myramistin against measles and mumps viruses. Antibiot Khimioter. 2005;50(5-6):17-9. Russian.
[Content Brief]
[4]. Agafonova MN, et al. Antibacterial activity profile of miramistin in in vitro and in vivo models. Microbial pathogenesis. 2020 May;142:104072. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.2773 mL | 11.3864 mL | 22.7728 mL | 56.9320 mL |
| 5 mM | 0.4555 mL | 2.2773 mL | 4.5546 mL | 11.3864 mL | |
| 10 mM | 0.2277 mL | 1.1386 mL | 2.2773 mL | 5.6932 mL | |
| 15 mM | 0.1518 mL | 0.7591 mL | 1.5182 mL | 3.7955 mL | |
| 20 mM | 0.1139 mL | 0.5693 mL | 1.1386 mL | 2.8466 mL | |
| 25 mM | 0.0911 mL | 0.4555 mL | 0.9109 mL | 2.2773 mL | |
| 30 mM | 0.0759 mL | 0.3795 mL | 0.7591 mL | 1.8977 mL | |
| 40 mM | 0.0569 mL | 0.2847 mL | 0.5693 mL | 1.4233 mL | |
| 50 mM | 0.0455 mL | 0.2277 mL | 0.4555 mL | 1.1386 mL | |
| 60 mM | 0.0380 mL | 0.1898 mL | 0.3795 mL | 0.9489 mL | |
| 80 mM | 0.0285 mL | 0.1423 mL | 0.2847 mL | 0.7117 mL | |
| 100 mM | 0.0228 mL | 0.1139 mL | 0.2277 mL | 0.5693 mL |
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