NKH477
Based on 1 Customer Validation
NKH477 (Colforsin dapropate hydrochloride) directly activates the catalytic unit of adenylate cyclase and increases intracellular cAMP. NKH477 is a forskolin derivative that improves cardiac failure mainly through its beneficial effects on diastolic cardiac function. NKH477 exerts an antiproliferative effect in vivo with an altered cytokine profile to inhibit the acute rejection of rat orthotopic lung allografts.
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- Purity: 99.86%
- CAS No.: 138605-00-2
- 화학식: C27H44ClNO8
- 분자량:546.09
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보관:
-20°C, protect from light, stored under nitrogen
* The compound is unstable in solutions, freshly prepared is recommended.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
Biological Activity
제품 설명
IC50 & Target
Adenylate Cyclase[1]
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Specific-pathogen-free inbred male Lewis rats (LEW) weighing 250 to 280 g[1]
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Dosage:1, 2 or 3 mg/kg/day
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Administration:Orally; daily; Days 3 and 5
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Result:Prolonged lung allograft survival in a dose dependent manner.
Chemical Information
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CAS No. 138605-00-2
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Appearance Solid
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분자량 546.09
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화학식 C27H44ClNO8
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Color White to off-white
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SMILES
O[C@@]12[C@@]3([C@@](C(C)(CC[C@@H]3O)C)([H])[C@H](OC(CCN(C)C)=O)[C@H](OC(C)=O)[C@]1(O[C@](C=C)(C)CC2=O)C)C.Cl
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Synonyms
Colforsin dapropate hydrochloride
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선적
Room temperature in continental US; may vary elsewhere.
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보관
-20°C, protect from light, stored under nitrogen
* The compound is unstable in solutions, freshly prepared is recommended.
용액&용해도
In Vitro:
DMSO : 100 mg/mL (183.12 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 20 mg/mL (36.62 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (9.16 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (9.16 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
순도&문서
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Data Sheet (282 KB)
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SDS (480 KB)
- English - EN (480 KB)
- Français - FR (480 KB)
- Deutsch - DE (480 KB)
- Norwegian - NO (480 KB)
- Español - ES (480 KB)
- Swedish - SV (480 KB)
- Italian - IT (480 KB)
- Korean - KR (480 KB)
- Portuguese - PT (480 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| H2O / DMSO | 1 mM | 1.8312 mL | 9.1560 mL | 18.3120 mL | 45.7800 mL |
| 5 mM | 0.3662 mL | 1.8312 mL | 3.6624 mL | 9.1560 mL | |
| 10 mM | 0.1831 mL | 0.9156 mL | 1.8312 mL | 4.5780 mL | |
| 15 mM | 0.1221 mL | 0.6104 mL | 1.2208 mL | 3.0520 mL | |
| 20 mM | 0.0916 mL | 0.4578 mL | 0.9156 mL | 2.2890 mL | |
| 25 mM | 0.0732 mL | 0.3662 mL | 0.7325 mL | 1.8312 mL | |
| 30 mM | 0.0610 mL | 0.3052 mL | 0.6104 mL | 1.5260 mL | |
| DMSO | 40 mM | 0.0458 mL | 0.2289 mL | 0.4578 mL | 1.1445 mL |
| 50 mM | 0.0366 mL | 0.1831 mL | 0.3662 mL | 0.9156 mL | |
| 60 mM | 0.0305 mL | 0.1526 mL | 0.3052 mL | 0.7630 mL | |
| 80 mM | 0.0229 mL | 0.1144 mL | 0.2289 mL | 0.5722 mL | |
| 100 mM | 0.0183 mL | 0.0916 mL | 0.1831 mL | 0.4578 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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