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Cat. No.: HY-N21777

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Petasiphenol

Petasiphenol is a phenolic natural product found in Petasites japonicus, possessing selective inhibitory activity against DNA polymerase λ. Petasiphenol binds to the N-terminal BRCT domain of DNA polymerase λ, with an IC₅₀ of 7.8 μM against DNA polymerase λ. Petasiphenol induces cell growth inhibition, G1 phase cell cycle arrest in human cancer cell lines, and exhibits anti-inflammatory activity against TPA-induced mouse ear inflammation. Petasiphenol can be used for research on cancer and inflammation-related diseases.

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CAS No.: 145904-50-3
화학식: C₁₈H₁₆O₇
분자량:344.32

보관:

Please store the product under the recommended conditions in the Certificate of Analysis.

Petasiphenol Chemical Structure

CAS No.: 145904-50-3

Amount: USD 0.00

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- 제품 설명
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
순도&문서
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

제품 설명

IC₅₀ & Target

^[1]

Human pol λ

7.6 μM (IC₅₀)

All DNA/RNA Synthesis Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
NUGC-3	LD₅₀	66 μM	Growth inhibitory activity against human NUGC-3 gastric cancer cells assessed as reduction in cell survival incubated for 48 hrs by MTT assay. Growth inhibitory activity against human NUGC-3 gastric cancer cells assessed as reduction in cell survival incubated for 48 hrs by MTT assay.	14599551
BALL-1	LD₅₀	48 μM	Growth inhibitory activity against human BALL-1 lymphoblastoid leukemia cells assessed as reduction in cell survival incubated for 48 hrs by MTT assay. Growth inhibitory activity against human BALL-1 lymphoblastoid leukemia cells assessed as reduction in cell survival incubated for 48 hrs by MTT assay.	14599551
MOLT-4	LD₅₀	48 μM	Growth inhibitory activity against human MOLT-4 lymphoblastic leukemia cells assessed as reduction in cell survival incubated for 48 hrs by MTT assay. Growth inhibitory activity against human MOLT-4 lymphoblastic leukemia cells assessed as reduction in cell survival incubated for 48 hrs by MTT assay.	14599551

In Vitro

Petasiphenol selectively and dose-dependently inhibits human DNA polymerase λ activity with an IC₅₀ of 7.8 µM^[1].
Petasiphenol selectively inhibits human DNA polymerase λ (IC₅₀ = 7.6 µM) without affecting other DNA polymerases or metabolic enzymes; it selectively binds to the N-terminal BRCT domain of human DNA polymerase λ, with a K_d of 7.99 µM for the isolated BRCT domain and a K_d of 8.15 µM for the full-length enzyme^[1].
Petasiphenol inhibits human DNA polymerase λ in a non-competitive manner with respect to both the DNA template and dNTP substrate, with K_i values of 3.8 µM and 4.5 µM, respectively^[1].
Petasiphenol binds to a pocket in the N-terminal BRCT domain of human DNA polymerase λ, interacting primarily through hydrogen bonds with the hydrophilic residues Gln76, Arg93, and Arg99^[1].
Petasiphenol (48 h) inhibits the growth of human NUGC-3 gastric cancer cells with an LD₅₀ of 66 μM; it inhibits the growth of human BALL-1 and MOLT-4 leukemia cell lines with LD₅₀ values of approximately 48 μM^[2].
Petasiphenol (66 μM; 48 h) arrests human NUGC-3 gastric cancer cells in the G1 phase of the cell cycle^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cycle Analysis^[2]

Cell Line:

NUGC-3
Concentration:

66 μM
Incubation Time:

48 h
Result:

Arrested NUGC-3 cells in the G1 phase, increasing the G1 phase population from 43.9% to 58.8% and decreasing the G2/M phase population from 14.3% to 10.0% compared to the control.

In Vivo

Petasiphenol (500 μg/ear) reduces TPA-induced ear edema in anti-inflammatory tests in mice, with an inhibition efficiency of 42%^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Related Protocols :

Chemical Information

CAS No. 145904-50-3
분자량 344.32
화학식 C₁₈H₁₆O₇
SMILES

O=C(OCC(=O)CC1=CC=C(O)C(O)=C1)C=CC2=CC=C(O)C(O)=C2
Structure Classification
- Phenols
- Polyphenols
Initial Source
선적

Room temperature in continental US; may vary elsewhere.
보관

Please store the product under the recommended conditions in the Certificate of Analysis.

순도&문서

Handling Instructions (2659 KB)

References

[1]. Mizushima Y, et al. Petasiphenol: A DNA Polymerase λ Inhibitor. Biochemistry, 2002, 41(49): 14463‑14471.

[2]. Mizushina Y, et al. Some anti-chronic inflammatory compounds are DNA polymerase lambda-specific inhibitors. Biochemical pharmacology. 2003 Nov 15;66(10):1935-44.

Calculators

몰농도 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

농도 희석 계산기

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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Keywords

MOQ

Minimum order quantity

100 mg

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