S29434
Based on 2 publication(s) in Google Scholar
S29434 (NMDPEF) is a potent, competitive, selective and cell-permeable inhibitor of quinone reductase 2 (QR2), with IC50s ranging from 5 to 16 nM for human QR2 at different organizational levels, and has good selectivity for QR2 over QR1. S29434 induces autophagy and inhibits QR2-mediated ROS production.
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- Purity: 99.29%
- CAS No.: 874484-20-5
- 화학식: C21H18N4O3
- 분자량:374.39
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보관:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
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Autophagy Compound Library
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Publications Citing Use of MedChemExpress (MCE) S29434
More
Biological Activity
IC50: 5 nM (h QR2, expressed in E. coli, NRH as co-substrate), 16 nM (h QR2, expressed in E. coli, with BNAH as co-substrate)[1]
S29434 (5-10 μM; 24 h) induces HepG2 cells autophagy[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HepG2 cell line
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Concentration:5 and 10 μM
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Incubation Time:24 hours
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Result:Mitochondrial ROS-independently and QR2-dependently triggered autophagy, and dose-dependently induced LC3-II which is a marker of autophagy vesicles.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:CH3 wild-type mice[1]
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Dosage:1 and 15 mg/kg
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Administration:Intraperitoneal injection; 1 and 15 mg/kg once
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Result:Positively affected memory by inhibiting QR2.
Chemical Information
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CAS No. 874484-20-5
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Appearance Solid
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분자량 374.39
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화학식 C21H18N4O3
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Color Light yellow to yellow
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SMILES
O=C(C1=CC=CO1)NCCC2=C(C3=NC=CC=C3C4)N4C5=CC=C(OC)N=C52
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Synonyms
NMDPEF
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (2)
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Journal Impact Factor
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Most Recent
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CNS Neurosci Ther
The influence of NQO2 on the dysfunctional autophagy and oxidative stress induced in the hippocampus of rats and in SH-SY5Y cells by fluoride. [Abstract]2023 Apr;29(4):1129-1141. PMID: 36650666 -
Int J Mol Sci
Neuroprotective Properties of Quinone Reductase 2 Inhibitor M-11, a 2-Mercaptobenzimidazole Derivative. [Abstract]2021 Dec 2;22(23):13061. PMID: 34884863
용액&용해도
DMSO : 19.29 mg/mL (51.52 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2 mg/mL (5.34 mM); Clear solution
This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
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Data Sheet (281 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.6710 mL | 13.3551 mL | 26.7101 mL | 66.7753 mL |
| 5 mM | 0.5342 mL | 2.6710 mL | 5.3420 mL | 13.3551 mL | |
| 10 mM | 0.2671 mL | 1.3355 mL | 2.6710 mL | 6.6775 mL | |
| 15 mM | 0.1781 mL | 0.8903 mL | 1.7807 mL | 4.4517 mL | |
| 20 mM | 0.1336 mL | 0.6678 mL | 1.3355 mL | 3.3388 mL | |
| 25 mM | 0.1068 mL | 0.5342 mL | 1.0684 mL | 2.6710 mL | |
| 30 mM | 0.0890 mL | 0.4452 mL | 0.8903 mL | 2.2258 mL | |
| 40 mM | 0.0668 mL | 0.3339 mL | 0.6678 mL | 1.6694 mL | |
| 50 mM | 0.0534 mL | 0.2671 mL | 0.5342 mL | 1.3355 mL |
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