Kulinone

Name: Kulinone
Brand: MedChemExpress
SKU: HY-116038
Rating: 4.5 (1 reviews)

Cat. No.: HY-116038 Purity: 99.75%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Kulinone has cytotoxic effects against A549, H460 and HGC27 with IC₅₀ values of 6.2, 7.8, 5.6 μg/mL.

For research use only. We do not sell to patients.

Kulinone Chemical Structure

CAS No. : 21688-61-9

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

Kulinone has cytotoxic effects against A549, H460 and HGC27 with IC₅₀ values of 6.2, 7.8, 5.6 μg/mL^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
BXPC-3	GI₅₀	3.61 μg/mL Compound: 9	Growth inhibition of human BxPC3 cells Growth inhibition of human BxPC3 cells	[PMID: 12502333]
DU-145	GI₅₀	3.6 μg/mL Compound: 9	Growth inhibition of human DU145 cells Growth inhibition of human DU145 cells	[PMID: 12502333]
KM-20L2	GI₅₀	3.6 μg/mL Compound: 9	Growth inhibition of human KM20L2 cells Growth inhibition of human KM20L2 cells	[PMID: 12502333]
MCF7	GI₅₀	3.6 μg/mL Compound: 9	Growth inhibition of human MCF7 cells Growth inhibition of human MCF7 cells	[PMID: 12502333]
MCF7	IC₅₀	42.5 μg/mL Compound: 13	Cytotoxicity against human MCF7 cells after 36 hrs by MTT assay Cytotoxicity against human MCF7 cells after 36 hrs by MTT assay	[PMID: 20961091]
NCI-H460	GI₅₀	3.6 μg/mL Compound: 9	Growth inhibition of human NCI-H460 cells Growth inhibition of human NCI-H460 cells	[PMID: 12502333]
P388	ED₅₀	1.7 μg/mL Compound: 13	Cytotoxicity against mouse P388 cells Cytotoxicity against mouse P388 cells	[PMID: 20961091]
P388	ED₅₀	4.6 μg/mL Compound: 9	Growth inhibition of mouse P388 cells Growth inhibition of mouse P388 cells	[PMID: 12502333]
SF-268	GI₅₀	3.6 μg/mL Compound: 9	Growth inhibition of human SF268 cells Growth inhibition of human SF268 cells	[PMID: 12502333]
U2OS	IC₅₀	6.9 μg/mL Compound: 13	Cytotoxicity against human U2OS cells after 36 hrs by MTT assay Cytotoxicity against human U2OS cells after 36 hrs by MTT assay	[PMID: 20961091]

Molecular Weight

440.70

Formula

C₃₀H₄₈O₂

CAS No.

21688-61-9

Appearance

Solid

Color

White to off-white

SMILES

C[C@@]12C3=CC[C@](C(C)(C(CC4)=O)C)([H])[C@@]4(C)[C@@]3([H])CC[C@]1([C@]([C@H](C2)O)([H])[C@H](C)CC/C=C(C)\C)C

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (113.46 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.2691 mL	11.3456 mL	22.6912 mL
5 mM	0.4538 mL	2.2691 mL	4.5382 mL
10 mM	0.2269 mL	1.1346 mL	2.2691 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (2.84 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 1.25 mg/mL (2.84 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.75%

Select Batch:

Data Sheet (271 KB) SDS (251 KB)

COA (249 KB) HNMR (268 KB) LCMS (87 KB)

Handling Instructions (2659 KB)

References

[1]. Wu SB, et al. Cytotoxic triterpenoids and steroids from the bark of Melia azedarach. Planta Med. 2011;77(9):922-928. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.2691 mL	11.3456 mL	22.6912 mL	56.7279 mL
	5 mM	0.4538 mL	2.2691 mL	4.5382 mL	11.3456 mL
	10 mM	0.2269 mL	1.1346 mL	2.2691 mL	5.6728 mL
	15 mM	0.1513 mL	0.7564 mL	1.5127 mL	3.7819 mL
	20 mM	0.1135 mL	0.5673 mL	1.1346 mL	2.8364 mL
	25 mM	0.0908 mL	0.4538 mL	0.9076 mL	2.2691 mL
	30 mM	0.0756 mL	0.3782 mL	0.7564 mL	1.8909 mL
	40 mM	0.0567 mL	0.2836 mL	0.5673 mL	1.4182 mL
	50 mM	0.0454 mL	0.2269 mL	0.4538 mL	1.1346 mL
	60 mM	0.0378 mL	0.1891 mL	0.3782 mL	0.9455 mL
	80 mM	0.0284 mL	0.1418 mL	0.2836 mL	0.7091 mL
	100 mM	0.0227 mL	0.1135 mL	0.2269 mL	0.5673 mL

Kulinone Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Kulinone21688-61-9OthersInhibitorinhibitorinhibit

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Kulinone

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Complete Stock Solution Preparation Table

Kulinone Related Classifications

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