2. Anti-infection Apoptosis
  3. Fungal Apoptosis
  4. Lawsone

Lawsone is an orally active naphthoquinone dye that can be isolated from the leaves of Lawsonia inermis. Lawsone can induce apoptosis. Lawsone has antibacterial, antitumor and antioxidant activities. Lawsone can be used in anti-tumor drug research.

For research use only. We do not sell to patients.

Lawsone Chemical Structure

Lawsone Chemical Structure

CAS No. : 83-72-7

Size Price Stock Quantity
Free Sample (0.1 - 0.5 mg)   Apply Now  
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 61 In-stock
Solution
10 mM * 1 mL in DMSO USD 61 In-stock
Solid
500 mg USD 55 In-stock
1 g   Get quote  
5 g   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Lawsone:

Lawsone Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Lawsone

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Lawsone is an orally active naphthoquinone dye that can be isolated from the leaves of Lawsonia inermis. Lawsone can induce apoptosis. Lawsone has antibacterial, antitumor and antioxidant activities. Lawsone can be used in anti-tumor drug research[1][2][3][4][5].

In Vitro

Lawsone (125, 250, 500 μM, 18 h) inhibits the growth of Escherichia coli in a dose-dependent manner[3].
Lawsone (0.5, 1, 1.5, 2 mg/mL, 48 h) inhibits cell proliferation in human DLD-1 cells by reducing NF-κB activity, resulting in inhibition of the expression levels of cyclin B1 and cdk1[4].
Lawsone (10, 20, 40, 80, 160 mg/mL, 24 h) has an anti-proliferation effect in SKOV-3 ovarian cancer cells and induces apoptosis by inhibiting Bcl-2[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Lawsone Related Antibodies

Cell Proliferation Assay[4]

Cell Line: DLD-1, MRC-5
Concentration: 0.5, 1, 1.5, 2 mg/mL
Incubation Time: 24-96 h
Result: Demonstrated significant suppression at concentration 2 mg/ml on DLD-1 cells and exhibited no suppression on normal fibroblast MRC-5 cells.

Western Blot Analysis[4]

Cell Line: DLD-1
Concentration: 0.5, 1, 1.5, 2 mg/mL
Incubation Time: 48 h
Result: Decreased the level of both proteins cyclin B1 and cdk1.
Exhibited a dose-dependent effect on translocation of p65 of cytosol to the nucleus.
In Vivo

Lawsone (62.5, 125, 250, 500, 750 μmol/kg/day, orally) induces hemolytic reactions associated with oxidative damage of red blood cells in rats[2].
Lawsone (200 mg/mL, orally, for 8 weeks) has a significant inhibitory effect on colon cancer in rats[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female rat model (10-11- week-old)[2]
Dosage: 62.5, 125, 250, 500, 750 μmol/kg/day
Administration: p.o.
Result: Increased the levels of ALT、AST、GLDH、LDH、HBDH、urea and creatinine at a dose of 500 or 750 μmol/kg/day.
Animal Model: Rat model of colon cancer[4]
Dosage: 200 mg/mL
Administration: p.o.
Result: Reduced the tumor diameters and decreased multiplicity of these adenomas.
Decreased the PCNA staining index
Molecular Weight

174.15

Formula

C10H6O3
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1C(O)=CC(C2=C1C=CC=C2)=O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (574.22 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.7422 mL 28.7109 mL 57.4218 mL
5 mM 1.1484 mL 5.7422 mL 11.4844 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (14.36 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (14.36 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.36%

COA (250 KB) HNMR (127 KB) RP-HPLC (221 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 5.7422 mL 28.7109 mL 57.4218 mL 143.5544 mL
5 mM 1.1484 mL 5.7422 mL 11.4844 mL 28.7109 mL
10 mM 0.5742 mL 2.8711 mL 5.7422 mL 14.3554 mL
15 mM 0.3828 mL 1.9141 mL 3.8281 mL 9.5703 mL
20 mM 0.2871 mL 1.4355 mL 2.8711 mL 7.1777 mL
25 mM 0.2297 mL 1.1484 mL 2.2969 mL 5.7422 mL
30 mM 0.1914 mL 0.9570 mL 1.9141 mL 4.7851 mL
40 mM 0.1436 mL 0.7178 mL 1.4355 mL 3.5889 mL
50 mM 0.1148 mL 0.5742 mL 1.1484 mL 2.8711 mL
60 mM 0.0957 mL 0.4785 mL 0.9570 mL 2.3926 mL
80 mM 0.0718 mL 0.3589 mL 0.7178 mL 1.7944 mL
100 mM 0.0574 mL 0.2871 mL 0.5742 mL 1.4355 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Lawsone Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Lawsone83-72-7FungalApoptosisAntitumorDLD-1SKOV-3Colon cancer rat modelInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Lawsone
Cat. No.:
HY-N2493
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

Request for HNMR Report

We have received your request and will respond to you as soon as possible.