LCA-H10 acetate
Based on 1 Customer Validation
LCA-H10 acetate is a bile acid-peptide lipid nanoparticle additive and endosomal escape inducer. LCA-H10 acetate acts through a histidine peptide-mediated proton sponge effect. LCA-H10 acetate interacts with siRNA, enabling encapsulation of siRNA in low-ionizable lipid lipid nanoparticles. LCA-H10 acetate enhances siRNA-mediated GFP gene silencing. LCA-H10 acetate can be used for research on prostate cancer.
For research use only. We do not sell to patients.
- Formula: C84H110N30O13·xC2H4O
- Molecular Weight:1747.97 (free base)
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| PC-3 | IC50 |
216.9 nM
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GFP gene silencing IC50 in human PC3-GFP cells assessed as reduction in GFP fluorescence after 5 hrs exposure with anti-GFP siRNA-containing ALC-0315-based LiLNP-LH and 43 hrs additional incubation.
GFP gene silencing IC50 in human PC3-GFP cells assessed as reduction in GFP fluorescence after 5 hrs exposure with anti-GFP siRNA-containing ALC-0315-based LiLNP-LH and 43 hrs additional incubation.
|
acs.bioconjchem.5c00303 |
| PC-3 | IC50 |
352.6 nM
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GFP gene silencing IC50 in human PC3-GFP cells assessed as reduction in GFP fluorescence after 5 hrs exposure with anti-GFP siRNA-containing SM102-based LiLNP-LH and 43 hrs additional incubation.
GFP gene silencing IC50 in human PC3-GFP cells assessed as reduction in GFP fluorescence after 5 hrs exposure with anti-GFP siRNA-containing SM102-based LiLNP-LH and 43 hrs additional incubation.
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acs.bioconjchem.5c00303 |
In Vitro
LCA-H10 (0.9 mol%; 4°C overnight) acetate achieves 83.7% siRNA encapsulation efficiency, a zeta potential of -16.0 mV, a pKa of 6.3, and an AFM height of 16.4 nm in LiLNP-LH[1].
LCA-H10 (2.0-10.0 × 1010 particles/mL; incubated at 37°C for 1 h) acetate exhibits pH-dependent hemolysis in LiLNP-LH, with a hemolysis rate of 16.1% at pH 6.0 and no hemolysis at pH 7.4[1].
LCA-H10 (24 h) acetate exhibits good biocompatibility in PC3 cells in LiLNP-LH[1].
LCA-H10 (5-43 h) acetate silences GFP in PC3-GFP cells in LiLNP-LH, with an IC50 value of 216.9 nM for ALC-0315-based LNPs and an IC50 value of 352.6 nM for SM102-based LNPs[1].
LCA-H10 (37°C incubation for 4 h) acetate promotes endosomal membrane destabilization in LiLNP-LH and the release of Calcein (HY-D0040) into the nucleus of PC3 cells, with a nuclear fluorescence intensity of 21.0[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Solid
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Molecular Weight 1747.97 (free base)
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Formula C84H110N30O13·xC2H4O
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SMILES
C[C@@]12[C@](CC[C@]2([H])[C@H](C)CCC(N[C@@H](CC3=CNC=N3)C(N[C@@H](CC4=CNC=N4)C(N[C@@H](CC5=CNC=N5)C(N[C@@H](CC6=CNC=N6)C(N[C@@H](CC7=CNC=N7)C(N[C@@H](CC8=CNC=N8)C(N[C@@H](CC9=CNC=N9)C(N[C@@H](CC%10=CNC=N%10)C(N[C@@H](CC%11=CNC=N%11)C(N[C@@H](CC%12=CNC=N%12)C(O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)([H])[C@@]%13([H])[C@]([C@@]%14([C@](C[C@H](O)CC%14)([H])CC%13)C)([H])CC1.CC(O)=O.[x]
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Sequence
LCA-His-His-His-His-His-His-His-His-His-His
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Sequence Shortening
LCA-HHHHHHHHHH
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
In Vitro:
H2O : ≥ 100 mg/mL
* "≥" means soluble, but saturation unknown.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
References
LCA-H10 acetate Related Classifications
HY-P11614A Related Classifications
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