2. Neuronal Signaling Apoptosis Immunology/Inflammation
  3. Amyloid-β Apoptosis NOD-like Receptor (NLR)
  4. Licochalcone B

Licochalcone B is an extract from the root of Glycyrrhiza uralensis. Licochalcone B inhibits amyloid β (42) self-aggregation (IC50=2.16 μM) and disaggregate pre-formed Aβ42 fibrils, reduce metal-induced Aβ42 aggregation through chelating metal ionsLicochalcone B inhibits phosphorylation of NF-κB p65 in LPS signaling pathway. Licochalcone B inhibits growth and induces apoptosis of NSCLC cells. Licochalcone B specifically inhibits the NLRP3 inflammasome by disrupting NEK7‐NLRP3 interaction.

For research use only. We do not sell to patients.

CAS No. : 58749-23-8

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10 mM * 1 mL in DMSO
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Customer Review

Based on 12 publication(s) in Google Scholar

Other Forms of Licochalcone B:

Licochalcone B Related Antibodies

Top Publications Citing Use of Products

    Licochalcone B purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Nov 22:150:157584.  [Abstract]

    Pronase E (1:2000-1000; 30 min) and Licochalcone B (20 μM; 30 min). The NCAM1 protein expression of drug affinity responsive target stability assay (upper panel) and statistical analysis (lower panel). Ns, not significant. **, P <0.01; ***, P <0.001; ****, P <0.0001.

    Licochalcone B purchased from MedChemExpress. Usage Cited in: EMBO Rep. 2022 Feb 3;23(2):e53499.  [Abstract]

    Cell Counting Kit 8 (CCK‐8) was used to assess the viability of BMDMs treated with different doses of Licochalcone B (LicoB) (10, 20, 30, 40, 50, 60, 70, 80 μM) for 24 h.

    Licochalcone B purchased from MedChemExpress. Usage Cited in: EMBO Rep. 2022 Feb 3;23(2):e53499.  [Abstract]

    Western blot analysis of cross-linked ASC in the Triton X-insoluble pellet from LPS-primed BMDMs pre-treated with Licochalcone B (LicoB) (20 μM) or vehicle and then stimulated with nigericin, poly(dA:dT) or Salmonella.

    Licochalcone B purchased from MedChemExpress. Usage Cited in: EMBO Rep. 2022 Feb 3;23(2):e53499.  [Abstract]

    TNF-α production in the supernatant (SN) was measured using ELISA treated with Licochalcone B (LicoB) (5, 10, 20, 40 μM) for 1 h (LicoB after LPS).

    Licochalcone B purchased from MedChemExpress. Usage Cited in: EMBO Rep. 2022 Feb 3;23(2):e53499.  [Abstract]

    Representative liver section images stained with H&E, Sirius Red and Masson’s trichrome stains treated with Licochalcone B (LicoB) (40 mg/kg, i.g.), MCC950 or a combination of LicoB and MCC950.

    Licochalcone B purchased from MedChemExpress. Usage Cited in: EMBO Rep. 2022 Feb 3;23(2):e53499.  [Abstract]

    Real-time quantitative PCR was used to detect the mRNA levels of Col1a1 treated with Licochalcone B (LicoB) (40 mg/kg, i.g.), MCC950 or a combination of LicoB and MCC950.

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    Description

    Licochalcone B is an extract from the root of Glycyrrhiza uralensis. Licochalcone B inhibits amyloid β (42) self-aggregation (IC50=2.16 μM) and disaggregate pre-formed Aβ42 fibrils, reduce metal-induced Aβ42 aggregation through chelating metal ionsLicochalcone B inhibits phosphorylation of NF-κB p65 in LPS signaling pathway. Licochalcone B inhibits growth and induces apoptosis of NSCLC cells. Licochalcone B specifically inhibits the NLRP3 inflammasome by disrupting NEK7‐NLRP3 interaction[1][2][3][4].

    IC50 & Target

    NLRP3

     

    Cellular Effect
    Cell Line Type Value Description References
    RAW264.7 IC50
    8.78 μM
    Compound: 2
    Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess reagent based assay
    Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess reagent based assay
    		[PMID: 28522265]
    RAW264.7 IC50
    9.94 μM
    Compound: 1, LicoB, Licochalcone B
    Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-stimulated nitric oxide production after 18 hrs by Griess assay
    Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-stimulated nitric oxide production after 18 hrs by Griess assay
    		[PMID: 24316124]
    RBL-2H3 IC50
    > 30 μM
    Compound: 1, LicoB, Licochalcone B
    Antiinflammatory activity in RBL2H3 cells assessed as inhibition of degranulation
    Antiinflammatory activity in RBL2H3 cells assessed as inhibition of degranulation
    		[PMID: 24316124]
    In Vitro

    Licochalcone B (IC50: 2.16 μM) inhibits self-mediated Aβ42 aggregation and disaggregates Aβ42 pre-formed fibrils[1].
    Licochalcone B (0-12 μM, 48 h) reduces the ROS generation and protects cells from H2O2-induced cell death in SH-SY5Y cells[1].
    Licochalcone B (10 μM, 1 h) inhibits LPS-induced NF-κB activation in RAW264.7 cells[2].
    Licochalcone B (10 μM, 1 h) inhibits the LPS-induced phosphorylation of NF-κB p65 at serine 276, and inhibited the expression of TNFα and MCP-1[2].
    Licochalcone B (0-20 μM, 24 or 48 h) inhibits human NSCLC cell growth[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Licochalcone B Related Antibodies

    Western Blot Analysis[2]

    Cell Line: RAW264.7 cells
    Concentration: 10 µM
    Incubation Time: 1 h
    Result: Inhibited the phosphorylation of NF-κB at serine 276 but not at serine 536.
    In Vivo

    Licochalcone B (1-25 mg/kg, oral gavage) alleviates oxidative stress of hepatotoxicity in mice induced by CCl4[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Mice induced by CCl4[5]
    Dosage: 1, 5, 25 mg/kg
    Administration: Oral gavage.
    Result: Elevated the levels of SOD and GSH, and reduced the GSSG levels.
    Molecular Weight

    286.28

    Formula

    C16H14O5
    CAS No.
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    O=C(C1=CC=C(O)C=C1)/C=C/C2=CC=C(O)C(O)=C2OC
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 83.33 mg/mL (291.08 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : < 0.1 mg/mL (insoluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.4931 mL 17.4654 mL 34.9308 mL
    5 mM 0.6986 mL 3.4931 mL 6.9862 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (7.27 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
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    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.91%

    SDS (393 KB)

    COA (246 KB) HNMR (226 KB) LCMS (111 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.4931 mL 17.4654 mL 34.9308 mL 87.3271 mL
    5 mM 0.6986 mL 3.4931 mL 6.9862 mL 17.4654 mL
    10 mM 0.3493 mL 1.7465 mL 3.4931 mL 8.7327 mL
    15 mM 0.2329 mL 1.1644 mL 2.3287 mL 5.8218 mL
    20 mM 0.1747 mL 0.8733 mL 1.7465 mL 4.3664 mL
    25 mM 0.1397 mL 0.6986 mL 1.3972 mL 3.4931 mL
    30 mM 0.1164 mL 0.5822 mL 1.1644 mL 2.9109 mL
    40 mM 0.0873 mL 0.4366 mL 0.8733 mL 2.1832 mL
    50 mM 0.0699 mL 0.3493 mL 0.6986 mL 1.7465 mL
    60 mM 0.0582 mL 0.2911 mL 0.5822 mL 1.4555 mL
    80 mM 0.0437 mL 0.2183 mL 0.4366 mL 1.0916 mL
    100 mM 0.0349 mL 0.1747 mL 0.3493 mL 0.8733 mL
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    Licochalcone B Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Licochalcone B58749-23-8Amyloid-βApoptosisNOD-like Receptor (NLR)β-amyloid peptideAβAbetaSH-SY5Y cellLPSRAW264.7 cellhepatotoxicityCCl4inflammasomeInhibitorinhibitorinhibit

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