Luotonin F
Luotonin F is an alkaloid found in the aerial parts of the P. nigellastrum Bunge. Luotonin F shows cytotoxic activity against mouse leukemia P-388 cells by inhibiting human topoisomerase II.
For research use only. We do not sell to patients.
- CAS No.: 244616-85-1
- Formula: C18H11N3O2
- Molecular Weight:301.30
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
IC50 & Target
[2]|
Topoisomerase II |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| P388 | IC50 |
20 μg/mL
Compound: 4
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Cytotoxic activity against mouse leukemia P-388 cells
Cytotoxic activity against mouse leukemia P-388 cells
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[PMID: 14980663] |
In Vitro
Luotonin F shows cytotoxic activity against mouse leukemia P-388 cells (IC50: 2.3 μg/mL)[1].
Luotonin F (25 μM) inhibits human topoisomerase II[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 244616-85-1
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Molecular Weight 301.30
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Formula C18H11N3O2
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SMILES
O=C1NC(C(C2=CC3=C(N=C2)C=CC=C3)=O)=NC4=CC=CC=C41
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
[1]. Liang JL, et al. Recent advances in the studies on luotonins. Molecules. 2011 Jun 14;16(6):4861-83. [Content Brief]
[2]. Ma Z, et al. Novel quinazoline-quinoline alkaloids with cytotoxic and DNA topoisomerase II inhibitory activities. Bioorg Med Chem Lett. 2004 Mar 8;14(5):1193-6. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Luotonin F Related Classifications
HY-118726 Related Classifications
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