2. PI3K/Akt/mTOR Stem Cell/Wnt Cell Cycle/DNA Damage Anti-infection Membrane Transporter/Ion Channel Autophagy
  3. GSK-3 CDK Parasite Proton Pump HSV Autophagy
  4. Manzamine A

Manzamine A, an orally active beta-carboline alkaloid, inhibits specifically GSK-3β and CDK-5 with IC50s of 10.2 μM and 1.5 μM, respectively. Manzamine A targets vacuolar ATPases and inhibits autophagy in pancreatic cancer cells. Manzamine A has antimalarial and anticancer activities. Manzamine A also shows potent activity against HSV-1.

At equivalent molar concentrations, both the salt and free forms of a compound exhibit comparable biological activity. Nevertheless, the salt form (Manzamine A hydrochloride) usually boasts enhanced water solubility and stability.

For research use only. We do not sell to patients.

Manzamine A Chemical Structure

Manzamine A Chemical Structure

CAS No. : 104196-68-1

Size Stock
1 mg Get quote
5 mg Get quote
10 mg Get quote
500 μg Get quote

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Other Forms of Manzamine A:

Manzamine A Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Manzamine A

Cell Viability Assay
Proliferation Assay

    Manzamine A purchased from MedChemExpress. Usage Cited in: Mar Drugs. 2023, 21(3), 151.

    Manzamine A (MA) shows a long-term ability to inhibit both MCF-7 and MDA-MB-231 cells growth. The MA (0, 2, 4 μM; 24 h)-treated cells are seeded in 6-well plates and then cultured for 2 weeks.

    Manzamine A purchased from MedChemExpress. Usage Cited in: Mar Drugs. 2023, 21(3), 151.

    Manzamine A (MA; 0, 0.5, 1, 2, 4, 8 μM; 24 h) shows cytotoxic effects on MDA-MB-231 and MCF-7 cells but is non-toxic to normal breast epithelial MCF-10A cells in a dose-dependent manner.

    View All GSK-3 Isoform Specific Products:

    View All Isoforms

    View All CDK Isoform Specific Products:

    View All Isoforms

    View All Parasite Isoform Specific Products:

    View All Isoforms

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Manzamine A, an orally active beta-carboline alkaloid, inhibits specifically GSK-3β and CDK-5 with IC50s of 10.2 μM and 1.5 μM, respectively. Manzamine A targets vacuolar ATPases and inhibits autophagy in pancreatic cancer cells. Manzamine A has antimalarial and anticancer activities. Manzamine A also shows potent activity against HSV-1[1][2][3][4].

    IC50 & Target[1][2][3]

    Plasmodium

     

    GSK-3β

    10.2 μM (IC50)

    CDK5

    1.5 μM (IC50)

    vacuolar ATPases

     

    Malaria

     

    HSV-1

     

    In Vitro

    Manzamine A (5-50 µM, 18 h) decreases tau phosphorylation, measured with ELISA[1].
    Manzamine A (10 µM) inhibits yeast S. cerevisiae growth by 30%[2].
    Manzamine A displays a few enlarged vacuoles in yeast[2].
    Manzamine A (2.5-10 µM, 24 h) increases acidity in pancreatic cancer cells and non-malignant Vero cells[2].
    Manzamine A (1 µM, 24 h) inhibits HSV-1 infection in SIRC cells[4].
    Manzamine A shows antimalarial activity with an IC50 of 8.0 nM (D6 clone) and 11 nM (W2 clone)[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Manzamine A Related Antibodies

    Cell Viability Assay[4]

    Cell Line: SIRC cell
    Concentration: 0.1, 0.5, 1, 2, 3, 5, and 10 µM
    Incubation Time: 72 h
    Result: Inhibited SIRC cell viability with an IC50 of 5.6 µM.
    In Vivo

    Manzamine A (50 and 100 mol/kg, p.o. or i.p.) inhibits the growth of the rodent malaria parasite Plasmodium berghei in infected mice[6].
    Manzamine A (8 mg/kg, i.p., daily for 8 consecutive days) prolongs the survival of SW mice to 20 days[7].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Plasmodium berghei in infected mice[6]
    Dosage: 50 or 100 mol/kg
    Administration: Intraperitoneal injection (i.p.) or oral administration (p.o.)
    Result: Inhibited the growth of the rodent malaria parasite Plasmodium berghei.
    Prolonged the survival of highly parasitaemic mice.
    Molecular Weight

    548.76

    Formula

    C36H44N4O
    CAS No.
    SMILES

    O[C@]1(CC/C=C\CCCC2)[C@@]3([H])[C@@](C[C@@]4([H])N3CCCC/C=C\4)(C[N@@]2CC5)[C@]5([H])C(C6=C(NC7=CC=CC=C87)C8=CC=N6)=C1
    Structure Classification
    Initial Source

    Okinawan marine sponge
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Please store the product under the recommended conditions in the Certificate of Analysis.
    Solvent & Solubility
    In Vitro: 

    DMSO : 10 mg/mL (18.22 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.8223 mL 9.1115 mL 18.2229 mL
    5 mM 0.3645 mL 1.8223 mL 3.6446 mL
    View the Complete Stock Solution Preparation Table

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation
    References

    Complete Stock Solution Preparation Table

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.8223 mL 9.1115 mL 18.2229 mL 45.5573 mL
    5 mM 0.3645 mL 1.8223 mL 3.6446 mL 9.1115 mL
    10 mM 0.1822 mL 0.9111 mL 1.8223 mL 4.5557 mL
    15 mM 0.1215 mL 0.6074 mL 1.2149 mL 3.0372 mL
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Manzamine A Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Manzamine A104196-68-1Keramamine AGSK-3CDKParasiteProton PumpHSVAutophagyGlycogen synthase kinase-3Glycogen synthase kinase 3Cyclin dependent kinaseHerpes simplex virusManzaminealkaloidAlzheimer'spancreaticcancerantimalarialInhibitorinhibitorinhibit

    Your Recently Viewed Products:

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Salutation

    Applicant Name *

    		 

    Email Address *

    Phone Number *

    		 

    Organization Name *

    Department *

    		 

    Requested quantity *

    Country or Region *

    		      

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    Manzamine A
    Cat. No.:
    HY-117025
    Quantity:
    MCE Japan Authorized Agent:

    Customer Review

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Lot #

    Applicant Name *

    		 

    Email Address *

    Phone Number

    		 

    Department *

    Organization Name *

    		 

    Country or Region *

    Remarks

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.