1. PI3K/Akt/mTOR Stem Cell/Wnt Cell Cycle/DNA Damage Anti-infection Membrane Transporter/Ion Channel Autophagy
  2. GSK-3 CDK Parasite Proton Pump HSV Autophagy
  3. Manzamine A

Manzamine A, an orally active beta-carboline alkaloid, inhibits specifically GSK-3β and CDK-5 with IC50s of 10.2 μM and 1.5 μM, respectively. Manzamine A targets vacuolar ATPases and inhibits autophagy in pancreatic cancer cells. Manzamine A has antimalarial and anticancer activities. Manzamine A also shows potent activity against HSV-1.

At equivalent molar concentrations, both the salt and free forms of a compound exhibit comparable biological activity. Nevertheless, the salt form (Manzamine A hydrochloride) usually boasts enhanced water solubility and stability.

For research use only. We do not sell to patients.

Manzamine A Chemical Structure

Manzamine A Chemical Structure

CAS No. : 104196-68-1

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Other Forms of Manzamine A:

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Manzamine A

Cell Viability Assay
Proliferation Assay

    Manzamine A purchased from MedChemExpress. Usage Cited in: Mar Drugs. 2023, 21(3), 151.

    Manzamine A (MA) shows a long-term ability to inhibit both MCF-7 and MDA-MB-231 cells growth. The MA (0, 2, 4 μM; 24 h)-treated cells are seeded in 6-well plates and then cultured for 2 weeks.

    Manzamine A purchased from MedChemExpress. Usage Cited in: Mar Drugs. 2023, 21(3), 151.

    Manzamine A (MA; 0, 0.5, 1, 2, 4, 8 μM; 24 h) shows cytotoxic effects on MDA-MB-231 and MCF-7 cells but is non-toxic to normal breast epithelial MCF-10A cells in a dose-dependent manner.
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    Description

    Manzamine A, an orally active beta-carboline alkaloid, inhibits specifically GSK-3β and CDK-5 with IC50s of 10.2 μM and 1.5 μM, respectively. Manzamine A targets vacuolar ATPases and inhibits autophagy in pancreatic cancer cells. Manzamine A has antimalarial and anticancer activities. Manzamine A also shows potent activity against HSV-1[1][2][3][4].

    IC50 & Target[1][2][3]

    Plasmodium

     

    GSK-3β

    10.2 μM (IC50)

    CDK5

    1.5 μM (IC50)

    vacuolar ATPases

     

    Malaria

     

    HSV-1

     

    In Vitro

    Manzamine A (5-50 µM, 18 h) decreases tau phosphorylation, measured with ELISA[1].
    Manzamine A (10 µM) inhibits yeast S. cerevisiae growth by 30%[2].
    Manzamine A displays a few enlarged vacuoles in yeast[2].
    Manzamine A (2.5-10 µM, 24 h) increases acidity in pancreatic cancer cells and non-malignant Vero cells[2].
    Manzamine A (1 µM, 24 h) inhibits HSV-1 infection in SIRC cells[4].
    Manzamine A shows antimalarial activity with an IC50 of 8.0 nM (D6 clone) and 11 nM (W2 clone)[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Viability Assay[4]

    Cell Line: SIRC cell
    Concentration: 0.1, 0.5, 1, 2, 3, 5, and 10 µM
    Incubation Time: 72 h
    Result: Inhibited SIRC cell viability with an IC50 of 5.6 µM.
    In Vivo

    Manzamine A (50 and 100 mol/kg, p.o. or i.p.) inhibits the growth of the rodent malaria parasite Plasmodium berghei in infected mice[6].
    Manzamine A (8 mg/kg, i.p., daily for 8 consecutive days) prolongs the survival of SW mice to 20 days[7].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Plasmodium berghei in infected mice[6]
    Dosage: 50 or 100 mol/kg
    Administration: Intraperitoneal injection (i.p.) or oral administration (p.o.)
    Result: Inhibited the growth of the rodent malaria parasite Plasmodium berghei.
    Prolonged the survival of highly parasitaemic mice.
    Molecular Weight

    548.76

    Formula

    C36H44N4O

    CAS No.
    SMILES

    O[C@]1(CC/C=C\CCCC2)[C@@]3([H])[C@@](C[C@@]4([H])N3CCCC/C=C\4)(C[N@@]2CC5)[C@]5([H])C(C6=C(NC7=CC=CC=C87)C8=CC=N6)=C1

    Structure Classification
    Initial Source

    Okinawan marine sponge

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    Please store the product under the recommended conditions in the Certificate of Analysis.

    Solvent & Solubility
    In Vitro: 

    DMSO : 10 mg/mL (18.22 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.8223 mL 9.1115 mL 18.2229 mL
    5 mM 0.3645 mL 1.8223 mL 3.6446 mL
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    Complete Stock Solution Preparation Table

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.8223 mL 9.1115 mL 18.2229 mL 45.5573 mL
    5 mM 0.3645 mL 1.8223 mL 3.6446 mL 9.1115 mL
    10 mM 0.1822 mL 0.9111 mL 1.8223 mL 4.5557 mL
    15 mM 0.1215 mL 0.6074 mL 1.2149 mL 3.0372 mL
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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Product Name:
    Manzamine A
    Cat. No.:
    HY-117025
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