1. PI3K/Akt/mTOR Stem Cell/Wnt Cell Cycle/DNA Damage Anti-infection Membrane Transporter/Ion Channel Autophagy
  2. GSK-3 CDK Parasite Proton Pump HSV Autophagy
  3. Manzamine A hydrochloride

Manzamine A hydrochloride  (Synonyms: Keramamine A hydrochloride)

Cat. No.: HY-117025A Purity: 99.66%
COA Handling Instructions

Manzamine A hydrochloride, an orally active beta-carboline alkaloid, inhibits specifically GSK-3β and CDK-5 with IC50s of 10.2 μM and 1.5 μM, respectively. Manzamine A hydrochloride targets vacuolar ATPases and inhibits autophagy in pancreatic cancer cells. Manzamine A hydrochloride has antimalarial and anticancer activities. Manzamine A hydrochloride also shows potent activity against HSV-1.

For research use only. We do not sell to patients.

Manzamine A hydrochloride Chemical Structure

Manzamine A hydrochloride Chemical Structure

CAS No. : 104264-80-4

Size Price Stock Quantity
1 mg USD 250 In-stock
5 mg USD 800 In-stock
10 mg   Get quote  
50 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of Manzamine A hydrochloride:

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Manzamine A hydrochloride

Cell Viability Assay
Proliferation Assay

    Manzamine A hydrochloride purchased from MedChemExpress. Usage Cited in: Mar Drugs. 2023, 21(3), 151.

    Manzamine A (MA) shows a long-term ability to inhibit both MCF-7 and MDA-MB-231 cells growth. The MA (0, 2, 4 μM; 24 h)-treated cells are seeded in 6-well plates and then cultured for 2 weeks.

    Manzamine A hydrochloride purchased from MedChemExpress. Usage Cited in: Mar Drugs. 2023, 21(3), 151.

    Manzamine A (MA; 0, 0.5, 1, 2, 4, 8 μM; 24 h) shows cytotoxic effects on MDA-MB-231 and MCF-7 cells but is non-toxic to normal breast epithelial MCF-10A cells in a dose-dependent manner.
    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Manzamine A hydrochloride, an orally active beta-carboline alkaloid, inhibits specifically GSK-3β and CDK-5 with IC50s of 10.2 μM and 1.5 μM, respectively. Manzamine A hydrochloride targets vacuolar ATPases and inhibits autophagy in pancreatic cancer cells. Manzamine A hydrochloride has antimalarial and anticancer activities. Manzamine A hydrochloride also shows potent activity against HSV-1[1][2][3][4].

    IC50 & Target[1][2][3]

    GSK-3β

    10.2 μM (IC50)

    Plasmodium

     

    CDK5

    1.5 μM (IC50)

    vacuolar ATPases

     

    Malaria

     

    HSV-1

     

    In Vitro

    Manzamine A (5-50 µM, 18 h) hydrochloride decreases tau phosphorylation, measured with ELISA[1].
    Manzamine A (10 µM) hydrochloride inhibits yeast S. cerevisiae growth by 30%[2].
    Manzamine A hydrochloride displays a few enlarged vacuoles in yeast[2].
    Manzamine A (2.5-10 µM, 24 h) hydrochloride increases acidity in pancreatic cancer cells and non-malignant Vero cells[2].
    Manzamine A (1 µM, 24 h) hydrochloride inhibits HSV-1 infection in SIRC cells[4].
    Manzamine A hydrochloride shows antimalarial activity with an IC50 of 8.0 nM (D6 clone) and 11 nM (W2 clone)[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Viability Assay[4]

    Cell Line: SIRC cell
    Concentration: 0.1, 0.5, 1, 2, 3, 5, and 10 µM
    Incubation Time: 72 h
    Result: Inhibited SIRC cell viability with an IC50 of 5.6 µM.
    In Vivo

    Manzamine A (50 and 100 mol/kg, p.o. or i.p.) hydrochloride inhibits the growth of the rodent malaria parasite Plasmodium berghei in infected mice[6].
    Manzamine A (8 mg/kg, i.p., daily for 8 consecutive days) hydrochloride prolongs the survival of SW mice to 20 days[7].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Plasmodium berghei in infected mice[6]
    Dosage: 50 or 100 mol/kg
    Administration: Intraperitoneal injection (i.p.) or oral administration (p.o.)
    Result: Inhibited the growth of the rodent malaria parasite Plasmodium berghei. Prolonged the survival of highly parasitaemic mice.
    Molecular Weight

    585.22

    Formula

    C36H45ClN4O

    CAS No.
    Appearance

    Solid

    Color

    Light yellow to brown

    SMILES

    O[C@]1(CC/C=C\CCCC2)[C@@]3([H])[C@@](C[C@@]4([H])N3CCCC/C=C\4)(C[N@@]2CC5)[C@]5([H])C(C6=C(NC7=CC=CC=C87)C8=CC=N6)=C1.Cl

    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    4°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 5.88 mg/mL (10.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.7088 mL 8.5438 mL 17.0876 mL
    5 mM 0.3418 mL 1.7088 mL 3.4175 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: 99.66%

    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.7088 mL 8.5438 mL 17.0876 mL 42.7190 mL
    5 mM 0.3418 mL 1.7088 mL 3.4175 mL 8.5438 mL
    10 mM 0.1709 mL 0.8544 mL 1.7088 mL 4.2719 mL
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Your Recently Viewed Products:

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product Name

     

    Salutation

    Applicant Name *

     

    Email Address *

    Phone Number *

     

    Organization Name *

    Department *

     

    Requested quantity *

    Country or Region *

         

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    Manzamine A hydrochloride
    Cat. No.:
    HY-117025A
    Quantity:
    MCE Japan Authorized Agent: