MAO-B-IN-55
Based on 1 Customer Validation
MAO-B-IN-55 (Compound 5c) is a reversible competitive MAO-B inhibitor with an IC50 of 2.9 nM, and it exhibits approximately 2750-fold higher selectivity for MAO-B over MAO-A. MAO-B-IN-55 can be used for the research of Parkinson's disease.
Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.
- Pureté : 98.10%
- CAS No.: 1458219-02-7
- Formule: C16H14BrNO2
- Masse moléculaire:332.20
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Stockage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Description
IC50 & Target
[1]|
MAO-B 2.9 nM (IC50) |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| PC-12 | Survival |
108.19 %
Compound: 28
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Cytotoxicity against rat PC12 cells assessed as cell survival at 20 uM after 72 hrs by MTT assay relative to control
Cytotoxicity against rat PC12 cells assessed as cell survival at 20 uM after 72 hrs by MTT assay relative to control
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[PMID: 27692996] |
| PC-12 | Survival |
111.71 %
Compound: 28
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Neuroprotective activity against 6-OHDA-induced toxicity in rat PC12 cells assessed as cell survival at 20 uM preincubated for 24 hrs followed by 6-OHDA addition measured after 24 hrs by MTT assay relative to control
Neuroprotective activity against 6-OHDA-induced toxicity in rat PC12 cells assessed as cell survival at 20 uM preincubated for 24 hrs followed by 6-OHDA addition measured after 24 hrs by MTT assay relative to control
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[PMID: 27692996] |
| PC-12 | Survival |
133.52 %
Compound: 28
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Neuroprotective activity against rotenone-induced toxicity in rat PC12 cells assessed as cell survival at 20 uM preincubated for 24 hrs followed by rotenone addition measured after 24 hrs by MTT assay relative to control
Neuroprotective activity against rotenone-induced toxicity in rat PC12 cells assessed as cell survival at 20 uM preincubated for 24 hrs followed by rotenone addition measured after 24 hrs by MTT assay relative to control
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[PMID: 27692996] |
| PC-12 | Survival |
80.24 %
Compound: 28
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Cytotoxicity against rat PC12 cells assessed as cell survival at 100 uM after 24 hrs by MTT assay relative to control
Cytotoxicity against rat PC12 cells assessed as cell survival at 100 uM after 24 hrs by MTT assay relative to control
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[PMID: 27692996] |
Chemical Information
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CAS No. 1458219-02-7
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Appearance Solid
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Masse moléculaire 332.20
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Formule C16H14BrNO2
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Color White to off-white
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SMILES
O=C1NC2=CC(OCC=3C=CC=C(Br)C3)=CC=C2CC1
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvant et solubilité
In Vitro:
DMSO : ≥ 125 mg/mL (376.28 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocole
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Human pluripotent stem cell midbrain dopaminergic neuron differentiation
Human pluripotent stem cells are directed toward midbrain dopaminergic neurons by first inducing a neural floor-plate-like progenitor state, then patterning cells with ventralizing SHH signaling and midbrain/WNT-FGF cues, and finally maturing progenitors into neurons expressing dopaminergic markers such as TH, NURR1/NR4A2, PITX3, DAT/SLC6A3, VMAT2/SLC18A2, GIRK2/KCNJ6, FOXA2, LMX1A, and EN1. The main readouts are loss of pluripotency, acquisition of FOXA2+/LMX1A+ midbrain floor-plate progenitors, emergence of βIII-tubulin+/MAP2+ neurons, and production of TH+ dopaminergic neurons with molecular, dopamine-release, and electrophysiological features of midbrain dopaminergic identity.
Pureté et documentation
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Fiche technique (269 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.0102 mL | 15.0512 mL | 30.1023 mL | 75.2559 mL |
| 5 mM | 0.6020 mL | 3.0102 mL | 6.0205 mL | 15.0512 mL | |
| 10 mM | 0.3010 mL | 1.5051 mL | 3.0102 mL | 7.5256 mL | |
| 15 mM | 0.2007 mL | 1.0034 mL | 2.0068 mL | 5.0171 mL | |
| 20 mM | 0.1505 mL | 0.7526 mL | 1.5051 mL | 3.7628 mL | |
| 25 mM | 0.1204 mL | 0.6020 mL | 1.2041 mL | 3.0102 mL | |
| 30 mM | 0.1003 mL | 0.5017 mL | 1.0034 mL | 2.5085 mL | |
| 40 mM | 0.0753 mL | 0.3763 mL | 0.7526 mL | 1.8814 mL | |
| 50 mM | 0.0602 mL | 0.3010 mL | 0.6020 mL | 1.5051 mL | |
| 60 mM | 0.0502 mL | 0.2509 mL | 0.5017 mL | 1.2543 mL | |
| 80 mM | 0.0376 mL | 0.1881 mL | 0.3763 mL | 0.9407 mL | |
| 100 mM | 0.0301 mL | 0.1505 mL | 0.3010 mL | 0.7526 mL |
MAO-B-IN-55 Related Classifications
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