MAPK-IN-5
MAPK-IN-5 is a potent MAPK inhibitor with an IC50 of 1.35 μM against HeLa cells. MAPK-IN-5 inhibits HeLa cell proliferation by inducing ROS-mediated DNA damage and mitochondrial apoptosis via the MAPK pathway. MAPK-IN-5 significantly inhibits colony formation, reduces the number of live cells, suppresses cell migration, and causes cell cycle arrest in the G2/M phase in HeLa cells. MAPK-IN-5 can be used for the study of cervical cancer.
For research use only. We do not sell to patients.
- CAS No.: 2579683-39-7
- Formula: C30H29F3N6O4
- Molecular Weight:594.58
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
-
Biological Activity
-
Chemical Information
- Purity & Documentation
- References
-
Help & FAQs
All Caspase Isoforms
More
Biological Activity
|
p38 |
Caspase-3 |
Caspase-9 |
Bcl-2 |
PARP-1 |
MAPK-IN-5 (Compound 3h) (5-10 μM, 48 h) significantly inhibits the colony formation of HeLa cells and increases the number of dead cells[1].
MAPK-IN-5 (5-10 μM, 0-48 h) has a pronounced inhibitory effect on the migration of HeLa cells[1].
MAPK-IN-5 (5-10 μM, 24 h) induces G2/M phase arrest in HeLa cells[1].
MAPK-IN-5 (5-10 μM, 48 h) induces apoptosis in HeLa cells, and the activation of the mitochondrial apoptosis pathway is involved[1].
MAPK-IN-5 (5-10 μM, 48 h) promotes ROS generation, triggers DNA damage and modulates the MAPK signaling pathway in HeLa cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:HeLa cells
-
Concentration:5 and 10 μM
-
Incubation Time:48 h
-
Result:Gradually decreased the number of live cells, while the number of dead cells increased.
Dose-dependently increased the intracellular ROS level.
Increased the fluorescence intensity of γ-H2AX in the nuclei of HeLa cells.
-
Cell Line:HeLa cells
-
Concentration:5 and 10 μM
-
Incubation Time:24 h
-
Result:Gradually decreased the proportion of cells in the G1 phase, while the proportion of cells in the G2/M phase significantly accumulated.
-
Cell Line:HeLa cells
-
Concentration:5 and 10 μM
-
Incubation Time:0, 24, 48 h
-
Result:Reduced the migration rate of HeLa cells was to 20.75% at 10 μM.
-
Cell Line:HeLa cells
-
Concentration:5 and 10 μM
-
Incubation Time:48 h
-
Result:Increased the total apoptosis rate of HeLa cells by 37.63% at 10 μM.
Caused cell shrinkage, intensified nuclear staining, and even apoptotic features such as nuclear condensation and fragmentation were observed.
-
Cell Line:HeLa cells
-
Concentration:5 and 10 μM
-
Incubation Time:48 h
-
Result:Decreased the levels of Bcl-2, Caspase-3, Caspase-9, and PARP-1.
Increased the levels of Bax and Cleaved PARP-1.
Dose-dependently inhibited the levels of phosphorylated ERK1/2 (p-ERK1/2) and phosphorylated JNK (p-JNK), while upregulating the phosphorylation of p38.
Had no effect on the total protein levels of ERK1/2, JNK, and p38.
Chemical Information
-
CAS No. 2579683-39-7
-
Molecular Weight 594.58
-
Formula C30H29F3N6O4
-
SMILES
COCCOC(C=C1N=CN=C2NC3=CC(C(N=N4)=CN4CC5=CC=C(C(F)(F)F)C=C5)=CC=C3)=C(C=C12)OCCOC
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz