Methyl cholate-d₅ 

Methyl cholate-d₅ is the deuterium labeled Methyl cholate. Methyl cholate is a bile acid analog and a specific inhibitor of TcdB toxin from Clostridioides difficile. Methyl cholate exerts a stronger selective inhibitory effect on TcdB than on TcdA. Methyl cholate induces conformational stabilization by binding to a unique site of TcdB, thereby blocking the binding of the toxin to host receptors and its self-processing process. Methyl cholate effectively protects human fibroblasts from TcdB-induced cytopathic effects. Methyl cholate exhibits dose-dependent anti-hepatic fibrosis activity in both cellular and zebrafish models, and significantly reduces the expression levels of α-SMA and COL-I. Methyl cholate is suitable for in-depth research in the fields of Clostridioides difficile infection and hepatic fibrosis^[1][2].

1. This compound can be used as a tracer.
2. This compound can be used as an internal standard for quantitative analysis by NMR, GC-MS, or LC-MS.

Stable heavy isotopes of hydrogen, carbon, and other elements have been incorporated into drug molecules, largely as tracers for quantitation during the drug development process. Deuteration has gained attention because of its potential to affect the pharmacokinetic and metabolic profiles of drugs^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

427.63

C₂₅H₃₇D₅O₅

52886-39-2

1448-36-8

O[C@@H]([C@]1([C@@]([H])([C@]23C)CC[C@@]3([C@@H](CCC(OC)=O)C)[H])[H])C[C@]4([H])C([2H])([C@@]([2H])(C([2H])(C[C@@]4([C@]1(C[C@@H]2O)[H])C)[2H])O)[2H]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Tam J, et al. Small molecule inhibitors of Clostridium difficile toxin B-induced cellular damage. Chem Biol. 2015;22(2):175-185.

  [2]. Deng K, Li M, Xiang L, et al. Integrated UHPLC-Q-exactive orbitrap HRMS and serum pharmacochemistry for the investigation of anti-hepatic fibrosis effect of Baoganning Decoction[J]. Phytomedicine, 2025, 137: 156363.