METTL16-IN-1
Based on 1 Customer Validation
METTL16-IN-1 is a potent METTL16 inhibitor with an IC50 value of 1.7 μM and a Kd value of 1.35 μM. Mettl16-in-1 inhibits the binding interaction of U6 snRNA deletion and METTL16 MTD with an IC50 value of 2.5 μM. METTL16-IN-1 has antitumor activity.
For research use only. We do not sell to patients.
- Purity: 99.22%
- Formula: C19H12BrN3O6S2
- Molecular Weight:522.35
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
METTL16-IN-1 (Compound 45) (12.5-50 μM; 24 h) promotes MAT2A splicing in MDA-MB-231 and A549 cells and increases the total m6A mRNA levels[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MDA-MB-231 cells
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Concentration:12.5, 25 and 50 μM
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Incubation Time:24 h
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Result:Increased the level of spliced MAT2A at 50 μM.
Chemical Information
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Appearance Solid
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Molecular Weight 522.35
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Formula C19H12BrN3O6S2
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Color Brown to reddish brown
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SMILES
O=C(O)C1=CC=C(S(=O)(NC(S/C2=C3C(N(C)C4=C\3C=C(Br)C=C4)=O)=NC2=O)=O)C=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
DMSO : 100 mg/mL (191.44 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (9.57 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.9144 mL | 9.5721 mL | 19.1443 mL | 47.8606 mL |
| 5 mM | 0.3829 mL | 1.9144 mL | 3.8289 mL | 9.5721 mL | |
| 10 mM | 0.1914 mL | 0.9572 mL | 1.9144 mL | 4.7861 mL | |
| 15 mM | 0.1276 mL | 0.6381 mL | 1.2763 mL | 3.1907 mL | |
| 20 mM | 0.0957 mL | 0.4786 mL | 0.9572 mL | 2.3930 mL | |
| 25 mM | 0.0766 mL | 0.3829 mL | 0.7658 mL | 1.9144 mL | |
| 30 mM | 0.0638 mL | 0.3191 mL | 0.6381 mL | 1.5954 mL | |
| 40 mM | 0.0479 mL | 0.2393 mL | 0.4786 mL | 1.1965 mL | |
| 50 mM | 0.0383 mL | 0.1914 mL | 0.3829 mL | 0.9572 mL | |
| 60 mM | 0.0319 mL | 0.1595 mL | 0.3191 mL | 0.7977 mL | |
| 80 mM | 0.0239 mL | 0.1197 mL | 0.2393 mL | 0.5983 mL | |
| 100 mM | 0.0191 mL | 0.0957 mL | 0.1914 mL | 0.4786 mL |
METTL16-IN-1 Related Classifications
HY-161402 Related Classifications
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