miR-21-IN-2

Cat. No.: HY-130487 Purity: 98.42%: Data Sheet
COA

SDS
Handling Instructions
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miR-21-IN-2 is a high-affinity miR-21 inhibitor. miR-21-IN-2 binds to the apical loop region of the pre-miR-21 hairpin, inhibits Dicer-mediated processing, and thereby blocks the biosynthesis of mature miR-21. miR-21-IN-2 can be used for the research of microRNA-related diseases.

For research use only. We do not sell to patients.

miR-21-IN-2 Chemical Structure

CAS No. : 304880-74-8

Size	Stock	Quantity
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Description

In Vitro

miR-21-IN-2 (Compound 1) (0.05-500 μM; 30 min) specifically binds to the 2-AP-labeled truncated 29-mer miR-21-hp RNA, with a K_d value of 2.3 μM as determined by 2-aminopurine fluorescence titration^[1].
miR-21-IN-2 (0-250 μM; 30 min) binds to the 5′-Cy5-labeled truncated 29-mer miR-21-hp RNA, and the K_d value is determined as 3.2 μM by fluorescence intensity assay^[1].
miR-21-IN-2 (0.1-100 μM; 0-2 h) inhibits Dicer-mediated cleavage of full-length pre-miR-21 RNA, with an inhibition rate of 47% at 1 μM, and exhibits dose-dependent activity in the range of 0.1 to 100 μM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

488.22

Formula

C₂₂H₂₀Br₂N₂O

CAS No.

304880-74-8

Appearance

Solid

Color

White to off-white

SMILES

BrC1=CC=C2C(C3=C(N2CC(CNC4=C(C)C=CC=C4)O)C=CC(Br)=C3)=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (204.83 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0483 mL	10.2413 mL	20.4826 mL
5 mM	0.4097 mL	2.0483 mL	4.0965 mL
10 mM	0.2048 mL	1.0241 mL	2.0483 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: 2.5 mg/mL (5.12 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.42%

Select Batch:

Data Sheet (275 KB) SDS (251 KB)

COA (253 KB) LCMS (331 KB)

Handling Instructions (2659 KB)

References

[1]. Connelly CM, et al. Discovery of Inhibitors of MicroRNA-21 Processing Using Small Molecule Microarrays. ACS Chem Biol. 2017;12(2):435-443.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.0483 mL	10.2413 mL	20.4826 mL	51.2064 mL
	5 mM	0.4097 mL	2.0483 mL	4.0965 mL	10.2413 mL
	10 mM	0.2048 mL	1.0241 mL	2.0483 mL	5.1206 mL
	15 mM	0.1366 mL	0.6828 mL	1.3655 mL	3.4138 mL
	20 mM	0.1024 mL	0.5121 mL	1.0241 mL	2.5603 mL
	25 mM	0.0819 mL	0.4097 mL	0.8193 mL	2.0483 mL
	30 mM	0.0683 mL	0.3414 mL	0.6828 mL	1.7069 mL
	40 mM	0.0512 mL	0.2560 mL	0.5121 mL	1.2802 mL
	50 mM	0.0410 mL	0.2048 mL	0.4097 mL	1.0241 mL
	60 mM	0.0341 mL	0.1707 mL	0.3414 mL	0.8534 mL
	80 mM	0.0256 mL	0.1280 mL	0.2560 mL	0.6401 mL
	100 mM	0.0205 mL	0.1024 mL	0.2048 mL	0.5121 mL

miR-21-IN-2 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

miR-21-IN-2304880-74-8MicroRNAmiRNAmiR-21 inhibitormicroRNA-related diseasesInhibitorinhibitorinhibit

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miR-21-IN-2

miR-21-IN-2 Related Antibodies

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