miR-21-IN-2
Based on 1 Customer Validation
miR-21-IN-2 is a high-affinity miR-21 inhibitor. miR-21-IN-2 binds to the apical loop region of the pre-miR-21 hairpin, inhibits Dicer-mediated processing, and thereby blocks the biosynthesis of mature miR-21. miR-21-IN-2 can be used for the research of microRNA-related diseases.
For research use only. We do not sell to patients.
- Purity: 98.42%
- CAS No.: 304880-74-8
- Formula: C22H20Br2N2O
- Molecular Weight:488.22
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
miR-21-IN-2 (Compound 1) (0.05-500 μM; 30 min) specifically binds to the 2-AP-labeled truncated 29-mer miR-21-hp RNA, with a Kd value of 2.3 μM as determined by 2-aminopurine fluorescence titration[1].
miR-21-IN-2 (0-250 μM; 30 min) binds to the 5′-Cy5-labeled truncated 29-mer miR-21-hp RNA, and the Kd value is determined as 3.2 μM by fluorescence intensity assay[1].
miR-21-IN-2 (0.1-100 μM; 0-2 h) inhibits Dicer-mediated cleavage of full-length pre-miR-21 RNA, with an inhibition rate of 47% at 1 μM, and exhibits dose-dependent activity in the range of 0.1 to 100 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 304880-74-8
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Appearance Solid
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Molecular Weight 488.22
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Formula C22H20Br2N2O
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Color White to off-white
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SMILES
BrC1=CC=C2C(C3=C(N2CC(CNC4=C(C)C=CC=C4)O)C=CC(Br)=C3)=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (204.83 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.0483 mL | 10.2413 mL | 20.4826 mL | 51.2064 mL |
| 5 mM | 0.4097 mL | 2.0483 mL | 4.0965 mL | 10.2413 mL | |
| 10 mM | 0.2048 mL | 1.0241 mL | 2.0483 mL | 5.1206 mL | |
| 15 mM | 0.1366 mL | 0.6828 mL | 1.3655 mL | 3.4138 mL | |
| 20 mM | 0.1024 mL | 0.5121 mL | 1.0241 mL | 2.5603 mL | |
| 25 mM | 0.0819 mL | 0.4097 mL | 0.8193 mL | 2.0483 mL | |
| 30 mM | 0.0683 mL | 0.3414 mL | 0.6828 mL | 1.7069 mL | |
| 40 mM | 0.0512 mL | 0.2560 mL | 0.5121 mL | 1.2802 mL | |
| 50 mM | 0.0410 mL | 0.2048 mL | 0.4097 mL | 1.0241 mL | |
| 60 mM | 0.0341 mL | 0.1707 mL | 0.3414 mL | 0.8534 mL | |
| 80 mM | 0.0256 mL | 0.1280 mL | 0.2560 mL | 0.6401 mL | |
| 100 mM | 0.0205 mL | 0.1024 mL | 0.2048 mL | 0.5121 mL |
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