Misetionamide
Based on 1 Customer Validation
Misetionamide is an orally oxathiazin-like compound. Misetionamide is a glyceraldehyde-3-phosphate dehydrogenase (GAPDH) inhibitor with antineoplastic activity. Misetionamide can be used for the research of cancer.
For research use only. We do not sell to patients.
- Purity: 99.49%
- CAS No.: 856785-75-6
- Formula: C3H7NO3S
- Molecular Weight:137.16
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Misetionamide (OTD) (0-2000 μM; 0-72 h) induces cell death in TNBC cells[2]. Misetionamide (OTD) (0-2000 μM; 12 h) inhibits TNBC cells proliferation[2]. Misetionamide (OTD) (0-1000 μM; 18 h) induces necrosis and apoptosis in TNBC cells[2]. Misetionamide (OTD) (250-1000 μM; 18 h) induces cell death by a ROS-dependent mechanism in TNBC cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:TNBC cells
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Concentration:0, 100, 200, 500, 1000 and 2000 μM
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Incubation Time:0, 24, 48 and 72 hours
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Result:Induced cell death in TNBC cells dose- and time-dependently.
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Cell Line:TNBC cells
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Concentration:0, 100, 200, 500, 1000 and 2000 μM
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Incubation Time:12 hours
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Result:Inhibited TNBC cell proliferation dose-dependently.
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Cell Line:TNBC cells
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Concentration:250, 500, 750, 1000 μM
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Incubation Time:24 hours
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Result:Induced TNBC cell death mainly dependent on ROS generation.
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Cell Line:TNBC cells
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Concentration:0, 100, 250, 750, 1000 μM
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Incubation Time:18 hours
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Result:Induced necrosis and apoptosis in TNBC cells dose-dependently and induced cell apoptosis is a underlying mechanism to kill TNBC cells.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 856785-75-6
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Appearance Solid
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Molecular Weight 137.16
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Formula C3H7NO3S
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Color White to off-white
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SMILES
O=S1(NCOCC1)=O
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Synonyms
GP-2250
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (729.08 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (18.23 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (278 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 7.2908 mL | 36.4538 mL | 72.9076 mL | 182.2689 mL |
| 5 mM | 1.4582 mL | 7.2908 mL | 14.5815 mL | 36.4538 mL | |
| 10 mM | 0.7291 mL | 3.6454 mL | 7.2908 mL | 18.2269 mL | |
| 15 mM | 0.4861 mL | 2.4303 mL | 4.8605 mL | 12.1513 mL | |
| 20 mM | 0.3645 mL | 1.8227 mL | 3.6454 mL | 9.1134 mL | |
| 25 mM | 0.2916 mL | 1.4582 mL | 2.9163 mL | 7.2908 mL | |
| 30 mM | 0.2430 mL | 1.2151 mL | 2.4303 mL | 6.0756 mL | |
| 40 mM | 0.1823 mL | 0.9113 mL | 1.8227 mL | 4.5567 mL | |
| 50 mM | 0.1458 mL | 0.7291 mL | 1.4582 mL | 3.6454 mL | |
| 60 mM | 0.1215 mL | 0.6076 mL | 1.2151 mL | 3.0378 mL | |
| 80 mM | 0.0911 mL | 0.4557 mL | 0.9113 mL | 2.2784 mL | |
| 100 mM | 0.0729 mL | 0.3645 mL | 0.7291 mL | 1.8227 mL |
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