Cat. No.: HY-122652

MitoBloCK-6

1 Cited Publications

Customer Review

Based on 1 publication(s) in Google Scholar

MitoBloCK-6 is a potent Erv1/ALR inhibitor, with an IC₅₀ of 900 nM and 700 nM, respectively. MitoBloCK-6 also inhibits Erv2 (IC₅₀=1.4 μM). MitoBloCK-6 can induce Apoptosis via cytochrome c release. MitoBloCK-6 inhibits growth of developing zebrafish motor neurons. MitoBloCK-6 has anticancer activity against liver cancer and leukemia.

For research use only. We do not sell to patients.

Purity: 98.0%
CAS No.: 303215-67-0
Formula: C₁₉H₁₄Cl₂N₂O
Molecular Weight:357.23

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

MitoBloCK-6 Chemical Structure

CAS No.: 303215-67-0

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (1)
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

IC₅₀ & Target

IC50: 900 nM (Erv1) 700 nM (ALR) 1.4 μM (Erv2)^[1]

In Vitro

MitoBloCK-6 (20 μM, 8 h) induces apoptosis via cytochrome c release in human embryonic stem cells (hESCs)^[1].
MitoBloCK-6 (20-40 μM, 72 h) inhibits proliferation of liver cancer cells McA-RH7777 via mitochondrial impairment^[2].
MitoBloCK-6 kills leukemia cell lines (OCI-AML2, TEX, Jurkat and NB4) with an IC₅₀ of 5-10 µM^[3].
MitoBloCK-6 (0.078-5 μM) increases the differentiation of OCI-AML2 and NB4 cells via inhibition of ALR, as increased surface expression of the myeloid markers CD11b^[4].
MitoBloCK-6 results in a significant decrease in the growth and branching of developing zebrafish motor neurons via inhibition of ALR^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis ^[1]

Cell Line:

human embryonic stem cells
Concentration:

20 µM
Incubation Time:

8 h
Result:

Resulted in a shift in cytochrome c localization from mitochondria into the cytosol.
Showed similar number of cells releasing cytochrome c.
Showed downstream events in apoptosis (PARP and caspase-3 cleavage).

In Vivo

MitoBloCK-6 (80 mg/kg, i.p., 5 of 7 days, twice daily) reduces tumor growth and the engraftment of AML cells into the marrow in mice xenografted with OCI-AML2 cells^[4].
MitoBloCK-6 (80 mg/kg i.p. 5 of 7 days, 2 weeks) strongly reduces the engraftment of primary AML cells in mice^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 303215-67-0
Appearance Solid
Molecular Weight 357.23
Formula C₁₉H₁₄Cl₂N₂O
Color Yellow to orange
SMILES

OC1=C(/C=N/C2=CC=C(NC3=CC=CC=C3)C=C2)C=C(Cl)C=C1Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (1)

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bioRxiv

Mitochondrial degradation of metallothionein enables local zinc mobilization during zinc limitation. [Abstract]

2026 Jun 10:2026.06.09.731205. PMID: 42327099

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (279.93 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.7993 mL

13.9966 mL

27.9932 mL

5 mM

0.5599 mL

2.7993 mL

5.5986 mL

10 mM

0.2799 mL

1.3997 mL

2.7993 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (274 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Dabir DV, et, al. A small molecule inhibitor of redox-regulated protein translocation into mitochondria. Dev Cell. 2013 Apr 15;25(1):81-92. [Content Brief]

[2]. Kabiri Y, et al. Mitochondrial Impairment by MitoBloCK-6 Inhibits Liver Cancer Cell Proliferation. Front Cell Dev Biol. 2021 Sep 20;9:725474. [Content Brief]

[3]. Jeyaraju D V, et al. Inhibition of the mitochondrial protein import machinery is selectively cytotoxic to acute myeloid leukemia (AML) cells and stem cells. Blood, 2016, 128(22): 1572.

[4]. Singh RP, et al. Disrupting Mitochondrial Copper Distribution Inhibits Leukemic Stem Cell Self-Renewal. Cell Stem Cell. 2020 Jun 4;26(6):926-937.e10. [Content Brief]

[5]. Johnson M E. One Fish, Two Fish, Small Fish, Huge Fish: Utilizing Zebrafish as a Model for Studying Mitochondrial Function. UCLA, 2012.

[6]. Jeyaraju D V, et al. Inhibiting the Mitochondrial Sulfhydryl Oxidase Alr Reduces Cox17 and Alters Mitochondrial Cristae Structure Leading to the Differentiation of AML and Stem Cells. 2017.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.7993 mL	13.9966 mL	27.9932 mL	69.9829 mL
	5 mM	0.5599 mL	2.7993 mL	5.5986 mL	13.9966 mL
	10 mM	0.2799 mL	1.3997 mL	2.7993 mL	6.9983 mL
	15 mM	0.1866 mL	0.9331 mL	1.8662 mL	4.6655 mL
	20 mM	0.1400 mL	0.6998 mL	1.3997 mL	3.4991 mL
	25 mM	0.1120 mL	0.5599 mL	1.1197 mL	2.7993 mL
	30 mM	0.0933 mL	0.4666 mL	0.9331 mL	2.3328 mL
	40 mM	0.0700 mL	0.3499 mL	0.6998 mL	1.7496 mL
	50 mM	0.0560 mL	0.2799 mL	0.5599 mL	1.3997 mL
	60 mM	0.0467 mL	0.2333 mL	0.4666 mL	1.1664 mL
	80 mM	0.0350 mL	0.1750 mL	0.3499 mL	0.8748 mL
	100 mM	0.0280 mL	0.1400 mL	0.2799 mL	0.6998 mL

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MitoBloCK-6

Apoptosis Compound Library

Metabolism/Protease Compound Library

Anti-Cancer Compound Library

Anti-Blood Cancer Compound Library

Glucose Metabolism Compound Library

Anti-Liver Cancer Compound Library

Metabolic Enzyme Compound Library

Cell Death Library

Anti-Hematopathy Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) MitoBloCK-6

Biological Activity

Chemical Information

Publications (1)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

MitoBloCK-6

Apoptosis Compound Library

Metabolism/Protease Compound Library

Anti-Cancer Compound Library

Anti-Blood Cancer Compound Library

Glucose Metabolism Compound Library

Anti-Liver Cancer Compound Library

Metabolic Enzyme Compound Library

Cell Death Library

Anti-Hematopathy Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) MitoBloCK-6

Biological Activity

Chemical Information

Publications (1)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

MitoBloCK-6 Related Classifications

Cat. No.: HY-122652