2. Cell Cycle/DNA Damage TGF-beta/Smad Epigenetics Anti-infection Apoptosis Metabolic Enzyme/Protease
  3. Topoisomerase PKC Orthopoxvirus Apoptosis Endogenous Metabolite
  4. Mitoxantrone diacetate

Mitoxantrone diacetate  (Synonyms: Mitozantrone diacetate; NSC 301739 diacetate)

Cat. No.: HY-13502B
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Mitoxantrone diacetate is a potent topoisomerase II inhibitor. Mitoxantrone diacetate also inhibits protein kinase C (PKC) activity with an IC50 of 8.5 μM. Mitoxantrone diacetate induces apoptosis of B-CLL (B-chronic lymphocytic leukaemia) cells. Mitoxantrone diacetate shows antitumor activity. Mitoxantrone diacetate also has anti-orthopoxvirus activity with EC50s of 0.25 μM and and 0.8 μM for cowpox and monkeypox, respectively.

For research use only. We do not sell to patients.

CAS No. : 70711-41-0

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Top Publications Citing Use of Products

46 Publications Citing Use of MCE Mitoxantrone diacetate

Cell Proliferation/Viability Assay
Apoptosis Analysis
IF
WB

    Mitoxantrone diacetate purchased from MedChemExpress. Usage Cited in: Exp Mol Med. 2025 Nov;57(11):2559-2573.  [Abstract]

    The USP11 inhibitor Mitoxantrone (5-20 μM; 24 h) was treated to A549 (western blotting for EGFR) cells. The EGFR protein levels were assessed by western blotting using an anti-EGFR antibody.

    Mitoxantrone diacetate purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2025 Aug 5;44(1):228.  [Abstract]

    U87-MG and U251 cells with stable CSRP2 overexpression (CSRP2) were treated with or without 0.5 µM Mitoxantrone (MTO) for indicated time periods and the cell proliferation was analyzed. Cells transfected with control vector (NC) were used as a negative control. Two-way ANOVA with Sidak’s post hoc test, n = 6 per group for U87-MG and U251.

    Mitoxantrone diacetate purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2025 Aug 5;44(1):228.  [Abstract]

    U87-MG and U251 cells with stable CSRP2 overexpression were treated with 0.5 µM Mitoxantrone (MTO) for 48 h and cell apoptosis was measured by flow cytometry

    Mitoxantrone diacetate purchased from MedChemExpress. Usage Cited in: Nat Cell Biol. 2024 Jun;26(6):917-931.  [Abstract]

    HeLa cells were pre-treated with DMSO or Mitoxantrone (10 μM, 1 h) followed by Tg treatment (1 μM, 3 h).

    Mitoxantrone diacetate purchased from MedChemExpress. Usage Cited in: Nat Cell Biol. 2024 Jun;26(6):917-931.  [Abstract]

    HeLa cells were pre-treated with DMSO or Mitoxantrone (10 μM, 1 h) followed by Tg treatment (1 μM, 3 h).

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    Topoisomerase Topo I Topo II DNA gyrase

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    PKC PKCα PKCβ PKCγ PKCδ PKCε PKCη PKCζ PKCθ PKCμ PKC-ι ℩ PKCι

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    Description

    Mitoxantrone diacetate is a potent topoisomerase II inhibitor. Mitoxantrone diacetate also inhibits protein kinase C (PKC) activity with an IC50 of 8.5 μM. Mitoxantrone diacetate induces apoptosis of B-CLL (B-chronic lymphocytic leukaemia) cells. Mitoxantrone diacetate shows antitumor activity[1][2][3][4]. Mitoxantrone diacetate also has anti-orthopoxvirus activity with EC50s of 0.25 μM and and 0.8 μM for cowpox and monkeypox, respectively[5].

    IC50 & Target

    Topoisomerase II

     

    PKC

    8.5 μM (IC50)

    In Vitro

    Mitoxantrone diacetate inhibits PKC in a competitive manner with respect to histone H1, and its Ki value is 6.3 μM and in a non-competitive manner with respect to phosphatidylserine and ATP[1].
    Mitoxantrone diacetate (0.5 μg/mL, 48 h) induces a decrease in B-CLL cells. Mitoxantrone diacetate induces DNA fragmentation and the proteolytic cleavage of poly(ADP-ribose) polymerase (PARP), demonstrating that the cytotoxic effect of Mitoxantrone diacetate is due to induction of apoptosis[2].
    Mitoxantrone diacetate shows cytotoxicity to human breast carcinoma cell lines MDA-MB-231 and MCF-7 with IC50 values of 18 and 196 nM, respectively[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Mitoxantrone diacetate Related Antibodies
    In Vivo

    Mitoxantrone diacetate (IP, 0-3.2 mg/kg/day) produces a statistically significant number of 60-day survivors at 1.6 mg/kg in mice with IP implanted L1210 leukemia[4].
    Mitoxantrone diacetate (IV, 0-3.2 mg/kg/day) shows effective antitumor activities and produces a 60% ILS (increase in lifespan) at 3.2 mg/kg in SC implanted Lewis lung carcinoma[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    564.58

    Formula

    C26H36N4O10
    CAS No.
    SMILES

    O=C1C2=C(C(NCCNCCO)=CC=C2NCCNCCO)C(C3=C(O)C=CC(O)=C13)=O.O=C(C)O.O=C(C)O
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Please store the product under the recommended conditions in the Certificate of Analysis.
    Purity & Documentation
    References
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    Mitoxantrone diacetate Related Classifications

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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Mitoxantrone diacetate70711-41-0Mitozantrone diacetate NSC 301739 diacetateTopoisomerasePKCOrthopoxvirusApoptosisEndogenous MetaboliteProtein kinase CB-CLL cellslymphocytesleukaemiaantitumorHL60 cellsbreast cancerInhibitorinhibitorinhibit

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