2. NF-κB Metabolic Enzyme/Protease Immunology/Inflammation
  3. Keap1-Nrf2 Reactive Oxygen Species (ROS)
  4. MSU38225

MSU38225 is a selective Nrf2 inhibitor. MSU38225 increases ROS levels by inhibiting antioxidant production. MSU38225 arrests cells at the G2/M phase, enhances cellular sensitivity to chemotherapeutic agents, and exerts synergistic effects with Carboplatin (HY-17393) and 5-fluorouracil (HY-90006). MSU38225 exhibits anticancer activity against lung cancer. MSU38225 can be used in research related to non-small cell lung cancer.

For research use only. We do not sell to patients.

MSU38225

MSU38225 Chemical Structure

CAS No. : 1629160-83-3

Size Price Stock Quantity
Free Sample (0.1 - 0.2 mg)   Apply Now  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
5 mg In-stock
10 mg In-stock
25 mg In-stock
50 mg In-stock
100 mg In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 Customer Validation

MSU38225 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

MSU38225 is a selective Nrf2 inhibitor. MSU38225 increases ROS levels by inhibiting antioxidant production. MSU38225 arrests cells at the G2/M phase, enhances cellular sensitivity to chemotherapeutic agents, and exerts synergistic effects with Carboplatin (HY-17393) and 5-fluorouracil (HY-90006). MSU38225 exhibits anticancer activity against lung cancer. MSU38225 can be used in research related to non-small cell lung cancer[1][2].

In Vitro

MSU38225 (24 h) inhibits tBHQ-induced Nrf2 transcriptional activity in MCF-7 NRF2/ARE reporter cells without cytotoxicity[1].
MSU38225 (24 h) suppresses the expression of Nrf2 downstream target genes and proteins in KEAP1-mutant A549 lung cancer cells without altering NFE2L2 mRNA levels[1].
MSU38225 (24 h) increases ROS production in tBHP-stimulated KEAP1-mutant A549 lung cancer cells in a dose-dependent manner via an Nrf2-dependent mechanism[1].
MSU38225 (72 h) inhibits the proliferation of cancer cells; it exerts stronger potency in Nrf2-dependent KEAP1-mutant A549 and H460 lung cancer cells via an Nrf2-dependent mechanism[1].
MSU38225 (7 days) exerts a dose-dependent and significant inhibitory effect on the 3D colony formation of A549, H460 and A427 lung cancer cells in soft agar[1].
MSU38225 (72 h) synergistically enhances the antiproliferative effects of Carboplatin (HY-17393) and 5-fluorouracil (HY-90006) in KEAP1G12C-mutant A549 lung cancer cells[1].
MSU38225 (10 μM; 24 h) reduces NRF2 protein levels in KEAP1-mutant non-small cell lung cancer cells A549 and H460, and significantly downregulates the mRNA expression of NRF2 target genes NQO1, GCLC, AKR1C2 and GCLM in A549 cells[2].
MSU38225 (10 μM; 24 h) arrests KEAP1-mutant non-small cell lung cancer cells A549 and H460 at the G2/M phase following 12 h of low-serum synchronization[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

MSU38225 Related Antibodies

Cell Proliferation Assay[2]

Cell Line: A549 human lung cancer cells
Concentration: 5 μM
Incubation Time: 72 h
Result: Inhibited cell proliferation by 9.8% relative to the vehicle control.

Western Blot Analysis[2]

Cell Line: A549 and H460 KEAP1-mutant non-small cell lung cancer cells
Concentration: 10 μM
Incubation Time: 24 h
Result: Decreased NRF2 protein levels in A549 and H460 cells.
Significantly reduced mRNA levels of NRF2 target genes NQO1, GCLC, AKR1C2, and GCLM in A549 cells relative to vehicle control.

Cell Migration Assay [2]

Cell Line: A549 human lung cancer cells
Concentration: 10 μM
Incubation Time: 24 h
Result: Significantly reduced (p < 0.0001) cell migration by 65% relative to vehicle control.
In Vivo

MSU38225 (50 mg/kg; i.p.; twice daily; 4 weeks) alone does not statistically significantly slow A549 xenograft tumor growth, but in combination with carboplatin, it significantly reduces tumor growth while maintaining good tolerability in Mus musculus[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: athymic nude mice (male, 6 weeks-old)[1]
Dosage: 50 mg/kg
Administration: i.p.; twice daily; 4 weeks
Result: Slowed tumor growth, though the change was not statistically significant.
Significantly slowed tumor growth when combined with carboplatin compared to vehicle control (p<0.01) and carboplatin alone (p<0.05).
Decreased Nrf2 protein expression in tumors compared to vehicle control.
Showed good tolerability with no changes in mouse body weight over time.
Molecular Weight

313.40

Formula

C21H19N3
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

N#CC1=C(NC2=CC(C)=CC(C)=C2)N=C(C)C(C3=CC=CC=C3)=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (319.08 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.1908 mL 15.9541 mL 31.9081 mL
5 mM 0.6382 mL 3.1908 mL 6.3816 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.1908 mL 15.9541 mL 31.9081 mL 79.7703 mL
5 mM 0.6382 mL 3.1908 mL 6.3816 mL 15.9541 mL
10 mM 0.3191 mL 1.5954 mL 3.1908 mL 7.9770 mL
15 mM 0.2127 mL 1.0636 mL 2.1272 mL 5.3180 mL
20 mM 0.1595 mL 0.7977 mL 1.5954 mL 3.9885 mL
25 mM 0.1276 mL 0.6382 mL 1.2763 mL 3.1908 mL
30 mM 0.1064 mL 0.5318 mL 1.0636 mL 2.6590 mL
40 mM 0.0798 mL 0.3989 mL 0.7977 mL 1.9943 mL
50 mM 0.0638 mL 0.3191 mL 0.6382 mL 1.5954 mL
60 mM 0.0532 mL 0.2659 mL 0.5318 mL 1.3295 mL
80 mM 0.0399 mL 0.1994 mL 0.3989 mL 0.9971 mL
100 mM 0.0319 mL 0.1595 mL 0.3191 mL 0.7977 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

MSU38225 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MSU382251629160-83-3MSU 38225MSU-38225Keap1-Nrf2Reactive Oxygen Species (ROS)non-small cell lung cancerH460 lung cancer cellsreactive oxygen speciesNrf2 pathwayKEAP1 mutationslung cancercarboplatinproteasomal degradationMCF-7 NRF2/ARE reporter cellsA549 lung cancer cellsInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
MSU38225
Cat. No.:
HY-153873
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (251 KB) Français - FR (251 KB) Deutsch - DE (251 KB) Norwegian - NO (251 KB) Español - ES (251 KB) Swedish - SV (251 KB) Italian - IT (251 KB) Korean - KR (251 KB) Portuguese - PT (251 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.