2BAct
Based on 4 publication(s) in Google Scholar
2BAct is a highly selective, orally active, and CNS-penetrant eIF2B (eukaryotic initiation factor 2B) activator with an EC50 of 33 nM. 2BAct prevents neurological defects caused by a chronic integrated stress response. 2BAct displays improved solubility and pharmacokinetics relative to eIF2B activator ISRIB trans-isomer (HY-12495).
For research use only. We do not sell to patients.
- Purity: 99.77%
- CAS No.: 2143542-28-1
- Formula: C19H16ClF3N4O3
- Molecular Weight:440.80
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Cell Cycle/DNA Damage Compound Library
HY-L004
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CNS-Penetrant Compound Library
HY-L028
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Anti-Aging Compound Library
HY-L034
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Orally Active Compound Library
HY-L061
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Targeted Diversity Library
HY-L099
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Highly Selective Activators Library
HY-L159
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Bioactive Compound Library Max
HY-L181
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MCE Bioactive Compound Library
HY-L001V
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Bioactive Compound Library
HY-L001
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High-Throughput Bioactive Compound Library
HY-L205
Publications Citing Use of MedChemExpress (MCE) 2BAct
More
Customer Validation & Images
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Others
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In Vivo Efficacy Study
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RT-PCR
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Others
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WB
Biological Activity
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eIF2 |
Primary fibroblast lysates from R191H embryos has lower GEF activity than WT lysates, and 2BAct enhances this activity threefold (EC50=7.3 nM)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
2BAct prevents the appearance of motor deficits, myelin loss and reactive gliosis in VWM mice[1].
2BAct stimulates the remaining activity of mutant eIF2B complex in vivo, abrogating the maladaptive stress response[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2143542-28-1
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Appearance Solid
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Molecular Weight 440.80
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Formula C19H16ClF3N4O3
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Color Light yellow to light brown
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SMILES
O=C(C(C=N1)=NC=C1C(F)F)NC(C2)(C3)CC23NC(COC(C=C4)=CC(F)=C4Cl)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (4)
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Journal Impact Factor
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Most Recent
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Sci Immunol
2026 Apr 3;11(118):eaeb6484. PMID: 41931597 -
Mol Cell
2025 Feb 20;85(4):756-769.e5. PMID: 39694034
2BAct purchased from MedChemExpress. Usage Cited in: Mol Cell. 2025 Feb 20;85(4):756-769.e5. [Abstract]
Primary neurons were treated with vehicle (gray) or DHPG (100 µM, 10 minutes) (purple) each in the presence of 2BAct (1 µM) and puromycylated for 5 minutes. PMY intensity was measured in PRPH-positive soma.
2BAct purchased from MedChemExpress. Usage Cited in: Mol Cell. 2025 Feb 20;85(4):756-769.e5. [Abstract]
WT female mice received an intrathecal injection of vehicle or 2BAct (1 µg) 60 minutes prior to intraplantar injection of DHPG (10 µg).
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Nat Cardiovasc Res
Cardiac adaptation to endurance exercise training requires suppression of GDF15 via PGC-1α. [Abstract]2025 Sep 24. PMID: 40993371
2BAct purchased from MedChemExpress. Usage Cited in: Nat Cardiovasc Res. 2025 Sep 24. [Abstract]
2BAct (5 μM). mRNA levels of Atf3 and Gdf15 in ISR manipulation experiments.
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J Biol Chem
2023 Apr;299(4):104595 PMID: 36898579
2BAct purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2023 Apr;299(4):104595 [Abstract]
cells expressing P(AAREx6)-Luc were pretreated with 1 μM A-92, 2 μM 2B-Act, or vehicle (Gcn2iB alone) for 30 min; then the indicated concentration of Gcn2iB was added and luciferase activity was measured after 6 h.
2BAct purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2023 Apr;299(4):104595 [Abstract]
HEK293 cells were pretreated with 1 μM A-92, 2 μM 2B-Act, or vehicle for 30 min and then 50 nM Gcn2iB was added for 6 h. Protein lysates were prepared and the levels of total and p-Gcn2 (T899), total and p-eIF2α (S51), Atf4, and actin were measured by immunoblot analyses.
Solvent & Solubility
DMSO : 100 mg/mL (226.86 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (11.34 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.2686 mL | 11.3430 mL | 22.6860 mL | 56.7151 mL |
| 5 mM | 0.4537 mL | 2.2686 mL | 4.5372 mL | 11.3430 mL | |
| 10 mM | 0.2269 mL | 1.1343 mL | 2.2686 mL | 5.6715 mL | |
| 15 mM | 0.1512 mL | 0.7562 mL | 1.5124 mL | 3.7810 mL | |
| 20 mM | 0.1134 mL | 0.5672 mL | 1.1343 mL | 2.8358 mL | |
| 25 mM | 0.0907 mL | 0.4537 mL | 0.9074 mL | 2.2686 mL | |
| 30 mM | 0.0756 mL | 0.3781 mL | 0.7562 mL | 1.8905 mL | |
| 40 mM | 0.0567 mL | 0.2836 mL | 0.5672 mL | 1.4179 mL | |
| 50 mM | 0.0454 mL | 0.2269 mL | 0.4537 mL | 1.1343 mL | |
| 60 mM | 0.0378 mL | 0.1891 mL | 0.3781 mL | 0.9453 mL | |
| 80 mM | 0.0284 mL | 0.1418 mL | 0.2836 mL | 0.7089 mL | |
| 100 mM | 0.0227 mL | 0.1134 mL | 0.2269 mL | 0.5672 mL |
2BAct Related Classifications
HY-125021 Related Classifications
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