(−)-N-Hydroxyapiosporamide
(−)-N-Hydroxyapiosporamide (NHAP), an alkaloid, is an NF-κB inhibitor with potent antitumor activity in vitro and in vivo. (−)-N-Hydroxyapiosporamide can be used for the research of colorectal cancer (CRC).
For research use only. We do not sell to patients.
- CAS No.: 1205676-02-3
- Formula: C24H31NO7
- Molecular Weight:445.51
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HCT-116 | IC50 |
0.93 μM
Compound: NHAP
|
Cytotoxicity against human HCT-116 cells assessed as decrease in cell viability incubated for 48 hrs by SRB assay
Cytotoxicity against human HCT-116 cells assessed as decrease in cell viability incubated for 48 hrs by SRB assay
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[PMID: 37243616] |
| HCT-116 | IC50 |
2.8 μM
Compound: NHAP
|
Cytotoxicity against human HCT-116 cells assessed as inhibition of cell growth incubated for 24 hrs by SRB assay
Cytotoxicity against human HCT-116 cells assessed as inhibition of cell growth incubated for 24 hrs by SRB assay
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[PMID: 37243616] |
| HCT-15 | IC50 |
0.57 μM
Compound: NHAP
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Cytotoxicity against human HCT-15 cells assessed as decrease in cell viability incubated for 48 hrs by SRB assay
Cytotoxicity against human HCT-15 cells assessed as decrease in cell viability incubated for 48 hrs by SRB assay
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[PMID: 37243616] |
| HT-29 | IC50 |
0.57 μM
Compound: NHAP
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Cytotoxicity against human HT-29 cells assessed as decrease in cell viability incubated for 48 hrs by SRB assay
Cytotoxicity against human HT-29 cells assessed as decrease in cell viability incubated for 48 hrs by SRB assay
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[PMID: 37243616] |
| HT-29 | IC50 |
0.61 μM
Compound: NHAP
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Cytotoxicity against human HT-29 cells assessed as inhibition of cell growth incubated for 24 hrs by SRB assay
Cytotoxicity against human HT-29 cells assessed as inhibition of cell growth incubated for 24 hrs by SRB assay
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[PMID: 37243616] |
| RKO | IC50 |
0.57 μM
Compound: NHAP
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Cytotoxicity against human RKO cells assessed as decrease in cell viability incubated for 48 hrs by SRB assay
Cytotoxicity against human RKO cells assessed as decrease in cell viability incubated for 48 hrs by SRB assay
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[PMID: 37243616] |
| SW480 | IC50 |
0.57 μM
Compound: NHAP
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Cytotoxicity against human SW480 cells assessed as decrease in cell viability incubated for 48 hrs by SRB assay
Cytotoxicity against human SW480 cells assessed as decrease in cell viability incubated for 48 hrs by SRB assay
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[PMID: 37243616] |
| SW-620 | IC50 |
0.57 μM
Compound: NHAP
|
Cytotoxicity against human SW620 cells assessed as decrease in cell viability incubated for 48 hrs by SRB assay
Cytotoxicity against human SW620 cells assessed as decrease in cell viability incubated for 48 hrs by SRB assay
|
[PMID: 37243616] |
Chemical Information
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CAS No. 1205676-02-3
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Molecular Weight 445.51
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Formula C24H31NO7
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SMILES
O=C([C@]1([C@]2([C@@]([H])(C[C@@H](CC2)C)C=C[C@H]1C)[H])[H])C3=C(C([C@@]4([C@H]5[C@@H]([C@@H](CC4)O)O5)O)=CN(O)C3=O)O
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Synonyms
NHAP
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Structure Classification
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Initial Source
Apiospora montagnei
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Research Protocol for Inflammation-related Diseases
The NLRP3 inflammasome is a cytosolic innate immune signaling platform that integrates priming signals and danger-signal activation to promote caspase-1 activation, maturation of IL-1β and IL-18, and gasdermin D-mediated pyroptotic cell death. The core experimental logic is to determine whether inflammatory disease phenotypes are driven by increased NLRP3 expression, ASC-containing inflammasome assembly, caspase-1 cleavage, GSDMD cleavage, and extracellular release of IL-1β/IL-18 rather than by nonspecific cell injury alone. The pathway is strongly linked to inflammation-related disease phenotypes because monosodium urate crystals activate NALP3/NLRP3 inflammasome signaling in gout-like crystal inflammation, cholesterol crystals activate NLRP3 inflammasomes in atherogenesis models, and DSS-induced intestinal inflammation has been reported to involve NLRP3 inflammasome activity. However, experimental colitis studies also show context-dependent protective effects of NLRP3 inflammasome co
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Subcutaneous Cell-Line-Derived Xenograft
Subcutaneous cell-line-derived xenograft (CDX) models are established by implanting cultured human cancer cell lines into immunodeficient mice, where the injected cells form localized tumors that can be monitored in vivo as a measure of tumorigenic potential, growth kinetics, and treatment response. These models are widely used in oncology research because they allow reproducible tumor formation and enable comparative assessment of tumor growth between different cell lines or genetic manipulations in a controlled in vivo microenvironment. Subcutaneous implantation of cancer cells in immunodeficient mice is a standard approach for evaluating tumor growth behavior and therapeutic response across multiple cancer types, including prostate, esophageal, pancreatic, and colon cancer models.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
(−)-N-Hydroxyapiosporamide Related Classifications
HY-N11777 Related Classifications
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