NCR247 

NCR247 is a defensin-like nodule cysteine-rich (NCR) peptide and haem sequesterer. NCR247 forms 6:6 haem:NCR247 hexamers and higher-order complexes to render haem and bound iron biologically inaccessible, induces iron-starvation response in Sinorhizobium meliloti and Medicago truncatula, and supports nitrogenase activity.NCR247 is critical for establishing nitrogen-fixing symbiosis between Sinorhizobium meliloti and Medicago truncatula, and is inactive against cytochrome haem.NCR247’s D-enantiomer binds and sequesters haem equivalently to its L-enantiomer.NCR247 can be used for the research of infections, periodontal disease, toxoplasmosis^[1].

NCR247 (~116-120 s) binds haem with nanomolar affinity, with cysteine residues, the C₁₀P₁₁ motif, and tyrosine residues contributing to high-affinity binding; the D-enantiomer and M. sativa ortholog retain equivalent nanomolar haem-binding affinity^[1].
NCR247 (5 μM; 16 min) sequesters haem and inhibits its intrinsic peroxidase activity, rendering the haem biologically inaccessible^[1].
NCR247 (2-4 μM; 30-60 min) induces an iron starvation response in S. meliloti by increasing expression of iron uptake genes and reducing expression of rirA; this response requires uptake of NCR247 into the bacterial cytoplasm via BacA and is mediated by the Irr regulatory pathway^[1].
NCR247 (2 μM; 30-60 min) drives increased iron import into S. meliloti, resulting in elevated total cellular iron content; this response is dependent on the Irr regulatory pathway^[1].
L-NCR247 and D-NCR247 (2.5-20 μM; 24-48 h) inhibit growth of haem-auxotrophic bacterial pathogens H. influenzae and P. gingivalis, with D-NCR247 more potent than L-NCR247; haem addition reverses this growth inhibition, confirming haem sequestration as the mechanism of action^[1].
L-NCR247 and D-NCR247 (25-150 μM; 6 h) inhibit Toxoplasma gondii plaque formation on human foreskin fibroblasts in a concentration-dependent manner, with haem binding required for the activity^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

NCR247 (endogenous expression; 2 μM; in vitro treatment) is essential for effective legume-rhizobium symbiosis, as its depletion reduces nodule iron content and disrupts normal nodule development, while exogenous NCR247 boosts iron uptake in symbiotic bacteroids^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

3005.49

C₁₂₄H₂₀₂N₄₈O₃₂S₄

1372669-36-7

Arg-Asn-Gly-Cys-Ile-Val-Asp-Pro-Arg-Cys-Pro-Tyr-Gln-Gln-Cys-Arg-Arg-Pro-Leu-Tyr-Cys-Arg-Arg-Arg (disulfide bridge:Cys4-Cys10, Cys15-Cys21)

RNGCIVDPRCPYQQCRRPLYCRRR (disulfide bridge:Cys4-Cys10, Cys15-Cys21)

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Sankari S, et al. A haem-sequestering plant peptide promotes iron uptake in symbiotic bacteria. Nat Microbiol. 2022;7(9):1453-1465.  [Content Brief]