NCR247 

NCR247 is a defensin-like nodule cysteine-rich (NCR) peptide and heme chelator. NCR247 induces iron starvation responses in Sinorhizobium meliloti and Medicago truncatula, and supports nitrogenase activity. NCR247 can be used in research related to periodontal disease and toxoplasmosis^[1].

NCR247 (~116-120 s) binds haem with nanomolar affinity, with cysteine residues, the C₁₀P₁₁ motif, and tyrosine residues contributing to high-affinity binding; the D-enantiomer and M. sativa ortholog retain equivalent nanomolar haem-binding affinity^[1].
NCR247 (5 μM; 16 min) sequesters haem and inhibits its intrinsic peroxidase activity, rendering the haem biologically inaccessible^[1].
NCR247 (2-4 μM; 30-60 min) induces an iron starvation response in S. meliloti by increasing expression of iron uptake genes and reducing expression of rirA; this response requires uptake of NCR247 into the bacterial cytoplasm via BacA and is mediated by the Irr regulatory pathway^[1].
NCR247 (2 μM; 30-60 min) drives increased iron import into S. meliloti, resulting in elevated total cellular iron content; this response is dependent on the Irr regulatory pathway^[1].
NCR247 (25-150 μM; 6 h) inhibit Toxoplasma gondii plaque formation on human foreskin fibroblasts in a concentration-dependent manner, with haem binding required for the activity^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

3005.49

C₁₂₄H₂₀₂N₄₈O₃₂S₄

1372669-36-7

Arg-Asn-Gly-Cys-Ile-Val-Asp-Pro-Arg-Cys-Pro-Tyr-Gln-Gln-Cys-Arg-Arg-Pro-Leu-Tyr-Cys-Arg-Arg-Arg (disulfide bridge:Cys4-Cys10, Cys15-Cys21)

RNGCIVDPRCPYQQCRRPLYCRRR (disulfide bridge:Cys4-Cys10, Cys15-Cys21)

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Sankari S, et al. A haem-sequestering plant peptide promotes iron uptake in symbiotic bacteria. Nat Microbiol. 2022;7(9):1453-1465.  [Content Brief]