NCX4040
Based on 1 Customer Validation
NCX4040 (NO-Aspirin), a non-steroidal anti-inflammatory drug (NSAID), is a nitric oxide (NO) releasing form of Aspirin. NCX4040 induces apoptosis in PC3 metastatic prostate cancer cells. NCX4040 has anti-inflammatory and anti-cancer effects.
For research use only. We do not sell to patients.
- Purity: 99.82%
- CAS No.: 287118-97-2
- Formula: C16H13NO7
- Molecular Weight:331.28
-
Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
-
Apoptosis Compound Library
HY-L003
-
Immunology/Inflammation Compound Library
HY-L007
-
Anti-Cancer Compound Library
HY-L025
-
Anti-Aging Compound Library
HY-L034
-
Oxygen Sensing Compound Library
HY-L045
-
Ferroptosis Compound Library
HY-L051
-
Human Metabolite Library
HY-L123
-
Anti-Prostate Cancer Compound Library
HY-L124
-
Cell Death Library
HY-L162
-
Bioactive Compound Library Max
HY-L181
-
MCE Bioactive Compound Library
HY-L001V
-
Bioactive Compound Library
HY-L001
-
Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library
HY-L199
-
High-Throughput Bioactive Compound Library
HY-L205
-
Mass Spectrometry Human Metabolite Library
HY-L215
Biological Activity
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
10 μM
Compound: p-NO-ASA
|
Cytotoxicity against human A549 cells assessed as growth inhibition after 48 hrs by trypan blue assay
Cytotoxicity against human A549 cells assessed as growth inhibition after 48 hrs by trypan blue assay
|
[PMID: 22001089] |
| BXPC-3 | IC50 |
10 μM
Compound: p-NO-ASA
|
Cytotoxicity against human BxPC3 cells assessed as growth inhibition after 48 hrs by trypan blue assay
Cytotoxicity against human BxPC3 cells assessed as growth inhibition after 48 hrs by trypan blue assay
|
[PMID: 22001089] |
| HCT-116 | IC50 |
68 μM
Compound: NCX-4040
|
Antiproliferative activity against human HCT116 cells after 72 hrs by cell counting kit analysis
Antiproliferative activity against human HCT116 cells after 72 hrs by cell counting kit analysis
|
[PMID: 21664138] |
| HCT-15 | IC50 |
10 μM
Compound: p-NO-ASA
|
Cytotoxicity against human HCT15 cells assessed as growth inhibition after 48 hrs by trypan blue assay
Cytotoxicity against human HCT15 cells assessed as growth inhibition after 48 hrs by trypan blue assay
|
[PMID: 22001089] |
| HT-29 | EC50 |
106.7 μM
Compound: 1
|
Apoptosis induction in human HT29 cells after 24 hrs
Apoptosis induction in human HT29 cells after 24 hrs
|
[PMID: 17441704] |
| HT-29 | IC50 |
10 μM
Compound: p-NO-ASA
|
Cytotoxicity against human HT-29 cells assessed as growth inhibition after 48 hrs by trypan blue assay
Cytotoxicity against human HT-29 cells assessed as growth inhibition after 48 hrs by trypan blue assay
|
[PMID: 22001089] |
| LNCaP | IC50 |
10 μM
Compound: p-NO-ASA
|
Cytotoxicity against human LNCAP cells assessed as growth inhibition after 48 hrs by trypan blue assay
Cytotoxicity against human LNCAP cells assessed as growth inhibition after 48 hrs by trypan blue assay
|
[PMID: 22001089] |
| MCF7 | IC50 |
>100 μM
Compound: NCX-4040
|
Antiproliferative activity against human MCF7 cells after 72 hrs by cell counting kit analysis
Antiproliferative activity against human MCF7 cells after 72 hrs by cell counting kit analysis
|
[PMID: 21664138] |
| MDA-MB-231 | IC50 |
14.4 μM
Compound: NCX-4040
|
Antiproliferative activity against human MDA-MB-231 cells after 72 hrs by cell counting kit analysis
Antiproliferative activity against human MDA-MB-231 cells after 72 hrs by cell counting kit analysis
|
[PMID: 21664138] |
| MIA PaCa-2 | IC50 |
10 μM
Compound: p-NO-ASA
|
Cytotoxicity against human MIAPaCa2 cells assessed as growth inhibition after 48 hrs by trypan blue assay
Cytotoxicity against human MIAPaCa2 cells assessed as growth inhibition after 48 hrs by trypan blue assay
|
[PMID: 22001089] |
| SCC-25 | IC50 |
10 μM
Compound: p-NO-ASA
|
Cytotoxicity against human SCC25 cells assessed as growth inhibition after 48 hrs by trypan blue assay
Cytotoxicity against human SCC25 cells assessed as growth inhibition after 48 hrs by trypan blue assay
|
[PMID: 22001089] |
| SK-BR-3 | IC50 |
30.5 μM
Compound: NCX-4040
|
Antiproliferative activity against human SK-BR-3 cells after 72 hrs by cell counting kit analysis
Antiproliferative activity against human SK-BR-3 cells after 72 hrs by cell counting kit analysis
|
[PMID: 21664138] |
| SW480 | IC50 |
19.1 μM
Compound: NCX-4040
|
Antiproliferative activity against human SW480 cells after 72 hrs by cell counting kit analysis
Antiproliferative activity against human SW480 cells after 72 hrs by cell counting kit analysis
|
[PMID: 21664138] |
| SW-620 | IC50 |
15.1 μM
Compound: NCX-4040
|
Antiproliferative activity against human SW620 cells after 72 hrs by cell counting kit analysis
Antiproliferative activity against human SW620 cells after 72 hrs by cell counting kit analysis
|
[PMID: 21664138] |
NCX4040 significantly induces hydrogen peroxide formation with ensuing oxidative stress and mitochondrial depolarization resulting in lipid peroxidation, cell cycle arrest, inhibition of colony growth and induction of apoptosis in PC3 cells[1].
NCX4040 (10-50 μM) inhibits migration potential of PC3 cells by depolymerizing F-actin and promoting anoikis[1].
NCX4040 down regulates cyclin D1 expression in PC3 cells. Western blot analysis revealed that significant down regulation of key anti-apoptotic markers such as cellular inhibitor of apoptosis protein-1 (cIAP1), X-linked inhibitor of apoptosis (XIAP), survivin, and Cellular-Myc (c-Myc)[1].
NCX4040-treated cells showes upregulation of phosho histone H2AX (pH2AX), cleaved caspase3 and cleaved Poly [ADP-ribose] polymerase 1 (PARP1)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 287118-97-2
-
Appearance Solid
-
Molecular Weight 331.28
-
Formula C16H13NO7
-
Color White to off-white
-
SMILES
O=C(OC1=CC=C(CO[N+]([O-])=O)C=C1)C2=CC=CC=C2OC(C)=O
-
Synonyms
NO-Aspirin
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (301.86 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
-
Data Sheet (272 KB)
-
SDS (699 KB)
- English - EN (699 KB)
- Français - FR (699 KB)
- Deutsch - DE (699 KB)
- Norwegian - NO (699 KB)
- Español - ES (699 KB)
- Swedish - SV (699 KB)
- Italian - IT (699 KB)
- Korean - KR (699 KB)
- Portuguese - PT (699 KB)
-
Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.0186 mL | 15.0930 mL | 30.1859 mL | 75.4649 mL |
| 5 mM | 0.6037 mL | 3.0186 mL | 6.0372 mL | 15.0930 mL | |
| 10 mM | 0.3019 mL | 1.5093 mL | 3.0186 mL | 7.5465 mL | |
| 15 mM | 0.2012 mL | 1.0062 mL | 2.0124 mL | 5.0310 mL | |
| 20 mM | 0.1509 mL | 0.7546 mL | 1.5093 mL | 3.7732 mL | |
| 25 mM | 0.1207 mL | 0.6037 mL | 1.2074 mL | 3.0186 mL | |
| 30 mM | 0.1006 mL | 0.5031 mL | 1.0062 mL | 2.5155 mL | |
| 40 mM | 0.0755 mL | 0.3773 mL | 0.7546 mL | 1.8866 mL | |
| 50 mM | 0.0604 mL | 0.3019 mL | 0.6037 mL | 1.5093 mL | |
| 60 mM | 0.0503 mL | 0.2515 mL | 0.5031 mL | 1.2577 mL | |
| 80 mM | 0.0377 mL | 0.1887 mL | 0.3773 mL | 0.9433 mL | |
| 100 mM | 0.0302 mL | 0.1509 mL | 0.3019 mL | 0.7546 mL |
Powered by Bioz