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NEP (Synonyms: VDP-green (NEP))

Cat. No.: HY-D1259
Handling Instructions

NEP (VDP-green (NEP)) is a turn-on fluorescent probe based on the intramolecular charge transfer (ICT) mechanism for sensing vicinal dithiol-containing proteins (VDPs). NEP is exhibits high selectivity toward VDPs in live cells and in vivo and displays a strong green fluorescence signal (λexem=430/535 nm). NEP has the potential for parkinsonism.

For research use only. We do not sell to patients.

NEP Chemical Structure

NEP Chemical Structure

CAS No. : 2414276-32-5

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Description

NEP (VDP-green (NEP)) is a turn-on fluorescent probe based on the intramolecular charge transfer (ICT) mechanism for sensing vicinal dithiol-containing proteins (VDPs). NEP is exhibits high selectivity toward VDPs in live cells and in vivo and displays a strong green fluorescence signal (λexem=430/535 nm). NEP has the potential for parkinsonism[1].

In Vitro

NEP (1-30 μM; 6 hours) has no or little cytotoxicity in HepG2 and PC12 cells[1].
NEP (10 μM; 4 hours) causes the fluorescence intensity to decrease gradually in PC12 cells pretreated with 6-OHDA (0, 50, 100, and 200 μM; for 30 min)[1].
NEP contains a dithiarsolane moiety (five-membered As-S ring) as the receptor of VDPs. In the presence of VDPs, NEP displays a strong green fluorescence signal produced by the cyclic dithiarsolane cleavage and subsequent intramolecular cyclization to liberate the fluorophore. NEP maintains a reliable fluorescence response within the range of pH 7-10[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

NEP (10 μM; for 4 h) causes the obvious green signal in zebrafishes (4 day old)[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

654.63

Formula

C₂₉H₃₁AsN₄O₅S₂

CAS No.

2414276-32-5

SMILES

O=C(N1CCCC)C2=C3C(C(NC(OCC4S[As](SC4)C5=CC=C(C=C5)NC(CCN)=O)=O)=CC=C3C1=O)=CC=C2

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Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

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Keywords:

NEPVDP-green (NEP)OthersintramolecularchargetransferICTvicinaldithiol-containingproteinsVDPsparkinsonismHepG2PC12Inhibitorinhibitorinhibit

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HY-D1259
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