Cat. No.: HY-116035

Nimbolide

2 Cited Publications

Customer Review

Based on 2 publication(s) in Google Scholar

Nimbolide is a triterpene compound that can be isolated from neem (Azadirachta indica), possesses anticancer and antiproliferative activity. Nimbolide induces tumor cell apoptosis by inhibiting NF-κB and CDK4/CDK6 kinase. Nimbolide suppresses the Wnt, PI3K-Akt, MAPK and JAK-STAT signaling pathways.

For research use only. We do not sell to patients.

Purity: 99.79%
CAS No.: 25990-37-8
Formula: C₂₇H₃₀O₇
Molecular Weight:466.52

Storage:

-20°C, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Nimbolide Chemical Structure

CAS No.: 25990-37-8

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (2)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]

NF-κB

CDK4

CDK6

All Wnt Isoforms :

All NF-κB Isoforms :

All CDK Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	15.56 μM Compound: 1	Antiproliferative activity against human A549 cell line Antiproliferative activity against human A549 cell line	[PMID: 16793266]
BC1 cell line	ED₅₀	0.39 μg/mL Compound: 1	Cytotoxicity against human BC1 cells after 72 hrs Cytotoxicity against human BC1 cells after 72 hrs	[PMID: 2614419]
Col2	ED₅₀	0.41 μg/mL Compound: 1	Cytotoxicity against human Col2 cells after 72 hrs Cytotoxicity against human Col2 cells after 72 hrs	[PMID: 2614419]
HOP-62	IC₅₀	10.37 μM Compound: 1	Antiproliferative activity against human HOP62 cell line Antiproliferative activity against human HOP62 cell line	[PMID: 16793266]
HT-1080	ED₅₀	0.31 μg/mL Compound: 1	Cytotoxicity against human HT1080 cells after 48 hrs Cytotoxicity against human HT1080 cells after 48 hrs	[PMID: 2614419]
HT-29	IC₅₀	6.94 μM Compound: 1	Antiproliferative activity against human HT29 cell line Antiproliferative activity against human HT29 cell line	[PMID: 16793266]
KB	ED₅₀	0.25 μg/mL Compound: 1	Cytotoxicity against human KB cells after 72 hrs Cytotoxicity against human KB cells after 72 hrs	[PMID: 2614419]
Lu1	ED₅₀	0.42 μg/mL Compound: 1	Cytotoxicity against human Lu1 cells Cytotoxicity against human Lu1 cells	[PMID: 2614419]
MCF7	IC₅₀	4 μM Compound: 11o	Antiproliferative activity against human MCF7 cells incubated for 24 hrs by MTT assay Antiproliferative activity against human MCF7 cells incubated for 24 hrs by MTT assay	[PMID: 33289552]
MDA-MB-231	IC₅₀	6 μM Compound: 11o	Antiproliferative activity against human MDA-MB-231 cells incubated for 24 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells incubated for 24 hrs by MTT assay	[PMID: 33289552]
OVCAR-5	IC₅₀	4.17 μM Compound: 1	Antiproliferative activity against human OVCAR5 cell line Antiproliferative activity against human OVCAR5 cell line	[PMID: 16793266]
P388	ED₅₀	0.065 μg/mL Compound: 1	Cytotoxicity against mouse P388 cells after 48 hrs Cytotoxicity against mouse P388 cells after 48 hrs	[PMID: 2614419]
PC-3	IC₅₀	4.59 μM Compound: 1	Antiproliferative activity against human PC3 cell line Antiproliferative activity against human PC3 cell line	[PMID: 16793266]
SK-MEL-2	ED₅₀	0.53 μg/mL Compound: 1	Cytotoxicity against human SK-MEL-2 cells after 72 hrs Cytotoxicity against human SK-MEL-2 cells after 72 hrs	[PMID: 2614419]
SW-60	IC₅₀	8.25 μM Compound: 1	Antiproliferative activity against human SW60 cell line Antiproliferative activity against human SW60 cell line	[PMID: 16793266]

In Vitro

Nimbolide (2 μM, 1-2 h) blocks growth factor-induced phosphorylation of Akt, ERK1/2, and STAT3 in U87EGFRvIII cells^[2].
Nimbolide (2-6 μM, 24 h) inhibits the IGF-I-mediated breast cancer cell proliferation in MCF-7/MDA-MB-231 cells^[3].
Nimbolide (2-6 μM, 24 h) inhibits proliferation and causes G0/G1-phase arrest through repression of cyclins (A1/B/C/D1/E1) in MCF-7/MDA-MB-231 cells^[3].
Nimbolide (0-6 μM, 30 min) increases the expression of DUSP4, increases E-cadherin, Snail and MMP-3 expression^[4].
Nimbolide (0-6 μM, 12 h) suppresses invasion and migration of NSCLC cells^[4].
Nimbolide (0-10 μM, 12 h) inhibits proliferation and induces apoptosis in colorectal cancer cells^[5].
Nimbolide (10 μM, 0-12 h) inhibits NF-κB activation in HCT-116/HT-29/Caco-2 cells by a time-dependent manner^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[2]

Cell Line:

U87EGFRvIII cells
Concentration:

5-20 μg/μL
Incubation Time:

2-12 h
Result:

Inhibited CDK4/6 activity in a dose-dependent manner.

Western Blot Analysis^[3]

Cell Line:

Breast cancer cell lines (MCF-7/MDA-MB-231)
Concentration:

2-4 μM for MCF-7 cells, 4-6 μM for MDA-MB-231 cells
Incubation Time:

24 h
Result:

Decreased protein levels of IGF-IRβ, IRS-2, PI3K, β-catenin, c-Myc, N-Ras, Raf-1, Mek, pERK1/2, cyclin (A1, B, C, D1 and E1) and PCNA.
Increased p38, p21, IGFBP-3 and PTEN protein expression.

Cell Viability Assay^[3]

Cell Line:

Breast cancer cell lines (MCF-7/MDA-MB-231)
Concentration:

2-4 μM for MCF-7 cells, 4-6 μM for MDA-MB-231 cells
Incubation Time:

24 h
Result:

Increased the G0/G1-phase population in MCF-7 cells by 68.43 and 62.06% at 2 and 4 μM, in MDA-MB-231 cells by 57.56 and 59.35% at 4 and 6 μM.
Increased the dead cells accumulation in MCF-7 cells by 7.62 and 22.91% at 2 and 4 μM, in MDA-MB-231 cells by 6.28 and 7.73% at 4 and 6 μM.

Western Blot Analysis^[5]

Cell Line:

Colorectal cancer cells (HCT-116/HT-29)
Concentration:

0-10 μM
Incubation Time:

12 h
Result:

Inhibited the expression of anti-apoptotic proteins (Bcl-2/Bcl-xL/c-IAP-1/survivin) and cell proliferative proteins (cyclin D1/c-Myc).
Inhibited the expression of proteins involved in tumor cell invasion, metastasis, and angiogenesis (MMP-9, ICAM-1, CXCR4, and VEGF) in a concentration-dependent manner.

In Vivo

Nimbolide (10 mg/kg, i.v., daily, 7 days) inhibits glioblastoma multiforme tumor growth in U87EGFRvⅢ xenografted mouse model^[2].
Nimbolide (5 mg/kg, 20 mg/kg; i.p.; daily, 10 days), inhibits tumor growth in HCT-116-derived xenograft mouse, reduces the expression of proteins involved in invasion, metastasis, and angiogenesis (MMP-9, CXCR4, ICAM-1, and VEGF)^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

HCT-116-derived xenograft mouse^[5]
Dosage:

5 mg/kg, 20 mg/kg
Administration:

Intraperitoneal injection (i.p.), daily, 10 days
Result:

Suppressed tumor growth in nude mice at a dose as low as 5 mg/kg.
Reduced tumor growth by almost 90% on day 10 at a dose as low as 20 mg/kg.
Didn’t affect the body weight of mice.

Chemical Information

CAS No. 25990-37-8
Appearance Solid
Molecular Weight 466.52
Formula C₂₇H₃₀O₇
Color White to off-white
SMILES

C[C@]12[C@@]3([H])[C@](OC([C@@]3(C=CC2=O)C)=O)([H])[C@]4([H])[C@]([C@@H]1CC(OC)=O)(C5=C(C)[C@@](C6=COC=C6)([H])C[C@@]5([H])O4)C
Structure Classification
- Terpenoids
- Triterpenes
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Publications (2)

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Journal Impact Factor

Phytomedicine

A novel METTL3 inhibitor nimbolide ameliorates osteoporosis via orchestrating osteoclastogenesis in an m6A-dependent manner. [Abstract]

2026 Jun:155:158048. PMID: 41865687
Ecotoxicol Environ Saf

Effects of the emerging contaminant 1,3,6,8-tetrabromocarbazole on the NF-κB and correlated mechanism in human hepatocellular carcinoma cells. [Abstract]

2022 Dec 1:247:114199. PMID: 36274317

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (107.18 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.1435 mL

10.7177 mL

21.4353 mL

5 mM

0.4287 mL

2.1435 mL

4.2871 mL

10 mM

0.2144 mL

1.0718 mL

2.1435 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 1 mg/mL (2.14 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 1 mg/mL (2.14 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (289 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Bodduluru LN, et al. Chemopreventive and therapeutic effects of nimbolide in cancer: the underlying mechanisms. Toxicol In Vitro. 2014 Aug;28(5):1026-35. [Content Brief]

[2]. Karkare S, et al. Direct inhibition of retinoblastoma phosphorylation by nimbolide causes cell-cycle arrest and suppresses glioblastoma growth. Clin Cancer Res. 2014 Jan 1;20(1):199-212. [Content Brief]

[3]. lumalai P, Arunkumar R, Benson CS, Sharmila G, Arunakaran J. Nimbolide inhibits IGF-I-mediated PI3K/Akt and MAPK signalling in human breast cancer cell lines (MCF-7 and MDA-MB-231). Cell Biochem Funct. 2014 Jul;32(5):476-84. [Content Brief]

[4]. Lin H, Qiu S, Xie L, Liu C, Sun S. Nimbolide suppresses non-small cell lung cancer cell invasion and migration via manipulation of DUSP4 expression and ERK1/2 signaling. Biomed Pharmacother. 2017 Aug;92:340-346. [Content Brief]

[5]. Gupta SC, Prasad S, Sethumadhavan DR, Nair MS, Mo YY, Aggarwal BB. Nimbolide, a limonoid triterpene, inhibits growth of human colorectal cancer xenografts by suppressing the proinflammatory microenvironment. Clin Cancer Res. 2013 Aug 15;19(16):4465-76. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.1435 mL	10.7177 mL	21.4353 mL	53.5883 mL
	5 mM	0.4287 mL	2.1435 mL	4.2871 mL	10.7177 mL
	10 mM	0.2144 mL	1.0718 mL	2.1435 mL	5.3588 mL
	15 mM	0.1429 mL	0.7145 mL	1.4290 mL	3.5726 mL
	20 mM	0.1072 mL	0.5359 mL	1.0718 mL	2.6794 mL
	25 mM	0.0857 mL	0.4287 mL	0.8574 mL	2.1435 mL
	30 mM	0.0715 mL	0.3573 mL	0.7145 mL	1.7863 mL
	40 mM	0.0536 mL	0.2679 mL	0.5359 mL	1.3397 mL
	50 mM	0.0429 mL	0.2144 mL	0.4287 mL	1.0718 mL
	60 mM	0.0357 mL	0.1786 mL	0.3573 mL	0.8931 mL
	80 mM	0.0268 mL	0.1340 mL	0.2679 mL	0.6699 mL
	100 mM	0.0214 mL	0.1072 mL	0.2144 mL	0.5359 mL

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Nimbolide

Apoptosis Compound Library

Cell Cycle/DNA Damage Compound Library

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Publications Citing Use of MedChemExpress (MCE) Nimbolide

All Wnt Isoforms

All NF-κB Isoforms

All CDK Isoforms

Biological Activity

Chemical Information

Publications (2)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Nimbolide Related Classifications

Cat. No.: HY-116035