TMA-DPH
Based on 11 publication(s) in Google Scholar
TMA-DPH is a hydrophobic fluorescent membrane probe (Ex=355 nm; Em=430 nm). TMA-DPH is able to anchor on the cell surface and localize to different regions of the phospholipid bilayer. By analyzing the fluorescence polarization values of TMA-DPH in the plasma membrane and membrane substructures, the fluidity of the cell membrane can be determined.
For research use only. We do not sell to patients.
- Purity: 99.60%
- CAS No.: 115534-33-3
- Formula: C28H31NO3S
- Molecular Weight:461.62
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Storage:
-20°C, sealed storage, away from moisture and light
* The compound is unstable in solutions, freshly prepared is recommended.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) TMA-DPH
More- Stem Cell Res Ther. 2024 Jan 8;15(1):12. [Abstract]
- J Med Chem. 2025 Dec 11;68(23):25307-25323. [Abstract]
- Ecotoxicol Environ Saf. 2023 Jan 1:249:114375. [Abstract]
- Ecotoxicol Environ Saf. 2021 Dec 20;227:112885. [Abstract]
- Biotechnol Biofuels. 2019 Mar 19:12:59. [Abstract]
- Food Funct. 2020 Jan 29;11(1):700-710. [Abstract]
- Pharmaceuticals. 2022 Aug 26;15(9):1059. [Abstract]
- Front Pharmacol. 2021 May 28:12:663743. [Abstract]
- Antimicrob Agents Chemother. 2026 Mar 4;70(3):e0139825. [Abstract]
- Sci Rep. 2025 Apr 8;15(1):12044. [Abstract]
- bioRxiv. 2025 Nov 29.
Customer Validation & Images
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Cell Imaging/Staining
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Cell Imaging/Staining
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Cell Imaging/Staining
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Cell Imaging/Staining
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Cell Imaging/Staining
Biological Activity
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Solution preparation
1.1 Preparation of stock solution
Solvent: DMSO
Concentration: 10 mM (optimized according to the experiment).
1.2 Preparation of working solution
Dilute to 0.5-5 μM with PBS or serum-free medium (pH 7.4) (optimized according to the experiment).
Note: The working solution should be prepared and used immediately. Keep it away from light.
2. Cell staining (suspended cells)
2.1 Collect cells by centrifugation and wash twice with PBS for 5 minutes each time.
2.2 TMA-DPH (0.5-5 μM) in Hanks and 20 mM Hepes buffer pH 7.4, and incubate it at 37°C, for 5-30 minutes (optimized according to the experiment).
2.3 Centrifuge at 400 g for 3-4 minutes and discard the supernatant.
2.4 Add PBS to wash the cells twice, 5 minutes each time.
2.5 Wash the cells and resuspended in the appropriate Hepes buffer pH 7.4.
2.6 Observe using a fluorescence microscope or flow cytometer.
3. Cell staining (adherent cells)
3.1 Culture the adherent cells on a sterile coverslip.
3.2 Remove the coverslip from the culture medium and remove the excess culture medium.
3.3 Add TMA-DPH (0.5-5 μM) in Hanks and 20 mM Hepes buffer pH 7.4, and incubate it at 37°C, for 5-30 minutes (optimized according to the experiment).
3.4 Wash the cells and resuspended in the appropriate Hepes buffer pH 7.4.
3.5 Fluorescence microscopy detection (Ex/Em = 355/430 nm).
Note: If flow cytometry is required, the cells need to be digested with trypsin and resuspended before staining.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
430
355
Chemical Information
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CAS No. 115534-33-3
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Appearance Solid
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Molecular Weight 461.62
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Formula C28H31NO3S
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Color Off-white to light yellow
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SMILES
C[N+](C)(C)C1=CC=C(/C=C/C=C/C=C/C2=CC=CC=C2)C=C1.[O-]S(=O)(C3=CC=C(C)C=C3)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture and light
* The compound is unstable in solutions, freshly prepared is recommended.
Publications (11)
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Journal Impact Factor
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Most Recent
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Stem Cell Res Ther
Uncovering impaired mitochondrial and lysosomal function in adipose-derived stem cells from obese individuals with altered biological activity. [Abstract]2024 Jan 8;15(1):12. PMID: 38185703
TMA-DPH purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2024 Jan 8;15(1):12. [Abstract]
Quantification of mitochondrial membrane fluidity of L-ADSCs and O-ADSCs by the fluorescence polarization (P) values of mitochondrial membrane measured by TMA-DPH (5 μM) dye. P value and membrane fluidity are negative correction (n ≥ 3).
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J Med Chem
2025 Dec 11;68(23):25307-25323. PMID: 41267343
TMA-DPH purchased from MedChemExpress. Usage Cited in: J Med Chem. 2025 Dec 11;68(23):25307-25323. [Abstract]
Membrane fluidity quantification using TMA-DPH fluorescence (24 h; n = 6).
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Ecotoxicol Environ Saf
Polystyrene nanoplastics promote the apoptosis in Caco-2 cells induced by okadaic acid more than microplastics. [Abstract]2023 Jan 1:249:114375. PMID: 36508836
TMA-DPH purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2023 Jan 1:249:114375. [Abstract]
The cell membrane fluidity was evaluated by TMA-DPH (0.5 μM, at 37 °C for 20 min).
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Ecotoxicol Environ Saf
Nanoplastics aggravate the toxicity of arsenic to AGS cells by disrupting ABC transporter and cytoskeleton. [Abstract]2021 Dec 20;227:112885. PMID: 34634601 -
Biotechnol Biofuels
QTL analysis reveals genomic variants linked to high-temperature fermentation performance in the industrial yeast. [Abstract]2019 Mar 19:12:59. PMID: 30923567
TMA-DPH purchased from MedChemExpress. Usage Cited in: Biotechnol Biofuels. 2019 Mar 19:12:59. [Abstract]
Membrane fluidity of the RHA strains of DAP1 at high temperature. The Membrane fluidity is determined by the steady-state anisotropy of fluorescent probe 1-[4-(trimethylamino)pheny]-6-phenyl-1,3,5-hexatriene (TMA-DPH, 42 °C).
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Food Funct
Vanillin enhances the passive transport rate and absorption of drugs with moderate oral bioavailability in vitro and in vivo by affecting the membrane structure. [Abstract]2020 Jan 29;11(1):700-710. PMID: 31909774 -
Pharmaceuticals
2022 Aug 26;15(9):1059. PMID: 36145280 -
Front Pharmacol
Benzaldehyde, A New Absorption Promoter, Accelerating Absorption on Low Bioavailability Drugs Through Membrane Permeability. [Abstract]2021 May 28:12:663743. PMID: 34122083
TMA-DPH purchased from MedChemExpress. Usage Cited in: Front Pharmacol. 2021 May 28:12:663743. [Abstract]
The changes in the fluorescence anisotropy of TMA-DPH (10 μM, at 37°C in dark for 20 min) in Caco-2 cells with different concentrations of benzaldehyde molecules. (●) HBSS, (■) 25 μM benzaldehyde, (▲) 100 μM benzaldehyde. Data are shown as the mean ± SD.
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Antimicrob Agents Chemother
Broxyquinoline enhances antibacterial activity of colistin and attenuates LPS-induced inflammation. [Abstract]2026 Mar 4;70(3):e0139825. PMID: 41631850 -
Sci Rep
PCYT2 mediates ovarian epithelial cancer metastasis by regulating cell membrane fluidity through the AMPK/FOXO1 signalling pathway. [Abstract]2025 Apr 8;15(1):12044. PMID: 40200068 -
Solvent & Solubility
DMSO : 10 mg/mL (21.66 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocol
L929 cells in 2 mL DM10F are allowed to adhere on microscope slide flasks. For the observation of plasma membrane labeling, cells are incubated for a short time (10 s) at room temperature with TMA-DPH 2×10-6 M in PBS or in DM10F from a 4×10-3 M stock solution in dimethylformamide. The unwashed slide is then transferred to the microscope and observed. For the labeling of internalized membrane, the cells are incubated in slide flasks at 37°C, with TMA-DPH 2×10-6 M in DM10F for the desired time, and then washed by gently shaking the slide in PBS for a few seconds[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (278 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Illinger D, et al. The kinetic aspects of intracellular fluorescence labeling with TMA-DPH support the maturation model for endocytosis in L929 cells. J Cell Biol. 1994 May;125(4):783-94. [Content Brief]
[2]. Illinger D, et al. A comparison of the fluorescence properties of TMA-DPH as a probe for plasma membrane and for endocytic membrane. Biochim Biophys Acta. 1995 Oct 4;1239(1):58-66. [Content Brief]
[3]. Benedetti A, et al. Plasma membrane fluidity in isolated rat hepatocytes: comparative study using DPH and TMA-DPH as fluorescent probes. J Gastroenterol Hepatol. 1989 May-Jun;4(3):221-7. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.1663 mL | 10.8314 mL | 21.6628 mL | 54.1571 mL |
| 5 mM | 0.4333 mL | 2.1663 mL | 4.3326 mL | 10.8314 mL | |
| 10 mM | 0.2166 mL | 1.0831 mL | 2.1663 mL | 5.4157 mL | |
| 15 mM | 0.1444 mL | 0.7221 mL | 1.4442 mL | 3.6105 mL | |
| 20 mM | 0.1083 mL | 0.5416 mL | 1.0831 mL | 2.7079 mL |
TMA-DPH Related Classifications
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