Methoxy-X04 (solution)
Methoxy-X04 (solution) is a fluorescent dye that crosses the blood-brain barrier and selectively binds to beta-pleated sheets found in dense core amyloid Aβ plaques. Methoxy-X04 retains in vitro binding affinity for amyloid b (Ab) fibrils (Ki= 26.8 nM). Methoxy-X04 is fluorescent and stains plaques, tangles, and cerebrovascular amyloid in postmortem sections of AD brain with good specificity.
Solvent and concentration: DMSO: 5 mg/mL
For research use only. We do not sell to patients.
- CAS No.: 863918-78-9
- Formula: C23H20O3
- Molecular Weight:344.41
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Solvent and concentration: DMSO: 5 mg/mL
1. Methoxy-X04 Solution Injection
24 hr prior to perfusion, weigh the mice and give each mouse an intraperitoneal injection of methoxy-X04 at a dose of 10 mg/kg of body weight.
2. Transcardial Perfusion of the Injected Mice
3. Brain Sectioning Using a Vibratome
4. Section Screening for the Presence of Methoxy-X04-stained Plaques
Examine each section successively, to avoid drying out the sections, using the following procedure:
Add a droplet of PBS to a microscope slide with a disposable pipette, and place the section on the droplet.
Examine the section under a fluorescent microscope (excitation 340-380 nm) to identify regions containing Methoxy-X04 labeled Aβ plaques.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 863918-78-9
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Molecular Weight 344.41
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Formula C23H20O3
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SMILES
COC1=CC(/C=C/C2=CC=C(O)C=C2)=CC=C1/C=C/C3=CC=C(O)C=C3
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Klunk WE, et al. Imaging Abeta plaques in living transgenic mice with multiphoton microscopy and methoxy-X04, a systemically administered Congo red derivative. J Neuropathol Exp Neurol. 2002 Sep;61(9):797-805. [Content Brief]
[2]. Bisht K, et al. Correlative Light and Electron Microscopy to Study Microglial Interactions with β-Amyloid Plaques. J Vis Exp. 2016 Jun 1;(112):54060. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Methoxy-X04 (solution) Related Classifications
HY-DY1045 Related Classifications
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