BCIP
Based on 1 Customer Validation
BCIP(BCIP p-toluidine salt; X-phosphate p-toluidine salt) is an artificial chromogenic substrate used for the sensitive colorimetric detection of alkaline phosphatase activity.
For research use only. We do not sell to patients.
- Purity: 99.93%
- CAS No.: 6578-06-9
- Formula: C15H15BrClN2O4P
- Molecular Weight:433.62
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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HY-15925
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HY-15930
TMB
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HY-116022A
4-Nitrophenyl phosphate disodium hexahydrate
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HY-D0994
4-Methylumbelliferyl phosphate
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HY-15435
CHAPS
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HY-15928
4-Nitrophenyl phosphate disodium
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HY-15915
DTNB
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HY-15934
X-GAL
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HY-12591B
D-Luciferin potassium
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HY-15930R
TMB (Standard)
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HY-12591
D-Luciferin sodium
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HY-15926
ONPG
Biological Activity
BCIP produces the blue-color enzymatic reaction product BCI during hydrolysis by ALP in HepG2 cell, which has strong SERRS activity and can be used for ALP activity detection[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 6578-06-9
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Appearance Solid
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Molecular Weight 433.62
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Formula C15H15BrClN2O4P
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Color White to off-white
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SMILES
ClC1=C(Br)C=CC2=C1C(OP(O)(O)=O)=CN2.NC3=CC=C(C)C=C3
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Synonyms
BCIP p-toluidine salt; X-phosphate p-toluidine salt
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
DMSO : 50 mg/mL (115.31 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
DMF : 25 mg/mL (57.65 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (277 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMF / DMSO | 1 mM | 2.3062 mL | 11.5308 mL | 23.0617 mL | 57.6542 mL |
| 5 mM | 0.4612 mL | 2.3062 mL | 4.6123 mL | 11.5308 mL | |
| 10 mM | 0.2306 mL | 1.1531 mL | 2.3062 mL | 5.7654 mL | |
| 15 mM | 0.1537 mL | 0.7687 mL | 1.5374 mL | 3.8436 mL | |
| 20 mM | 0.1153 mL | 0.5765 mL | 1.1531 mL | 2.8827 mL | |
| 25 mM | 0.0922 mL | 0.4612 mL | 0.9225 mL | 2.3062 mL | |
| 30 mM | 0.0769 mL | 0.3844 mL | 0.7687 mL | 1.9218 mL | |
| 40 mM | 0.0577 mL | 0.2883 mL | 0.5765 mL | 1.4414 mL | |
| 50 mM | 0.0461 mL | 0.2306 mL | 0.4612 mL | 1.1531 mL | |
| DMSO | 60 mM | 0.0384 mL | 0.1922 mL | 0.3844 mL | 0.9609 mL |
| 80 mM | 0.0288 mL | 0.1441 mL | 0.2883 mL | 0.7207 mL | |
| 100 mM | 0.0231 mL | 0.1153 mL | 0.2306 mL | 0.5765 mL |
BCIP Related Classifications
HY-15909 Related Classifications
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