Papain (lyophilized powder, ≥10 units/mg protein)

This product requires activation before use. Activation method:
Incubate the enzyme in a solution containing 1.1 mM EDTA, 0.067 mM mercaptoethanol, and 5.5 mM cysteine-HCl for 30 minutes.

Papain (lyophilized powder, ≥10 units/mg protein) (5 U/mL; 15 min) pretreatment of O Rh-positive red cells enhances anti-D-mediated agglutination, while it slightly reduces anti-A-mediated agglutination of A Rh-negative red cells^[2].
Papain (lyophilized powder, ≥10 units/mg protein) (5 U/mL; 15 min) pretreatment of O Rh-positive red cells increases their sensitivity to lymphocyte-mediated lysis in anti-D-mediated ADCC assays at low antibody concentrations and a 15:1 effector-to-target ratio^[2].
Papain (lyophilized powder, ≥10 units/mg protein) (5 U/mL; 15 min) pretreatment of O Rh-positive target red cells enables significant PBMC-mediated lysis in anti-D-mediated ADCC assays at a 1:3:3 effector-to-target-to-competitor ratio^[2].
Papain (lyophilized powder, ≥10 units/mg protein) (5 U/mL; 15 min) pretreatment of A Rh-negative target red cells enables significant PBMC-mediated lysis in anti-A-mediated ADCC assays at a 1:3:3 effector-to-target-to-competitor ratio^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.