Enzyme Substrates

DABCYL-Glu-Arg-Nle-Phe-Leu-Ser-Phe-Pro-EDANS is a fluorescent dye, and can be applied in a fluorogenic substrate for an aspartyl proteinase from human malaria parasite.

Suc-Gly-Gly-Phe-pNA is the chymotrypsin substrate with an K_m value of 1.6 mM.

[pSer2, pSer5, pSer7]-CTD, a substrate for CDK7 (cyclin dependent protein kinase), is a phosphorylated polypeptide at ser2, ser5 and ser7 sites of RNA polymerase II carboxy-terminal domain (CTD).

H-D-Phe-Pip-Arg-pNA dihydrochloride (S-2238 dihydrochloride) is a specific chromogenic peptide substrate for thrombin (Thrombin). The amide bond of H-D-Phe-Pip-Arg-pNA dihydrochloride is rapidly hydrolyzed by thrombin, producing a characteristic yellow absorbance at 405 nm, and the change in absorbance is proportional to thrombin activity. H-D-Phe-Pip-Arg-pNA dihydrochloride can be used in biochemical detection-related studies.

pp60 (v-SRC) Autophosphorylation Site, Phosphorylated is the phosphorylated peptide of an EGFR substrate. pp60 (v-SRC) Autophosphorylation Site, Phosphorylated can be used for the screening of EGFR Kinase inhibitors via phosphorylated-substrate quantification.

Bacterial Sortase Substrate III, Abz/DNP is a fluorescent peptide substrate. Bacterial Sortase Substrate III, Abz/DNP undergoes cleavage catalyzed by Staphylococcus aureus sortase A (SrtAΔN24) and Streptococcus pyogenes sortase A (SrtAΔN81), and catalyzes the formation of an amide bond between the carboxyl group of threonine and the amino group of the cell wall cross-bridge. Cleavage of this substrate can be detected at Ex/Em=320 nm/420 nm.

[pSer2, pSer5, pSer7]-CTD (TFA), a substrate for CDK7 (cyclin dependent protein kinase), is a phosphorylated polypeptide at ser2, ser5 and ser7 sites of RNA polymerase II carboxy-terminal domain (CTD).

SNAP-25 (187-203), a peptide corresponding to residues 187–203 of SNAP-25, is a substrate for botulinum neurotoxin (BoNT)/A and can be used as a substrate for quantifying the activity of BoNT/C1(1-430) .

Syk Kinase Peptide Substrate is a Syk kinase peptide substrate.

Syk Kinase Peptide Substrate, Biotin labeled is a biotin-labled Syk kinase peptide substrate.

2B-(SP) is a eIF2B-based substrate for glycogen synthase kinase-3 (GSK-3). 2B-(SP) is readily phosphorylated by both the α and β isoforms of GSK-3.

2B-(SP) TFA is a eIF2B-based substrate for glycogen synthase kinase-3 (GSK-3). 2B-(SP) TFA is readily phosphorylated by both the α and β isoforms of GSK-3.

Phospho-Glycogen Synthase Peptide-2 (substrate) is peptide substrate for glycogen synthase kinase-3 (GSK-3) and can be used for affinity purification of protein-serine kinases.

Akt/SKG Substrate Peptide is a synthetic peptide suitable as a substrate for Akt/PKB, which is not phosphorylated by p70S6K or MAPK1.

Akt/SKG Substrate Peptide TFA is a synthetic peptide suitable as a substrate for Akt/PKB, which is not phosphorylated by p70S6K or MAPK1.

HIV Protease Substrate 1, a fiuorogenic HIV protease substrate, can be used to study enzymatic activity of HIV protease.

HIV Protease Substrate 1 TFA, a fiuorogenic HIV protease substrate, can be used to study enzymatic activity of HIV protease.

RETF-4NA, a chymase-specific substrate, is a sensitive and selective substrate for chymase when free or bound to α₂M.

DAPK Substrate Peptide is a synthetic peptide substrate for death associated protein kinase (DAPK), with a K_m of 9 μM.

[pTyr5] EGFR (988-993) TFA is derived from the autophosphorylation site (Tyr992) of epidermal growth factor receptor (EGFR 988-993). [pTyr5] EGFR (988-993) TFA is often complexed with the catalytically inactive protein-tyrosine phosphate 1B (PTP1B).